We narrowed to 11,748 results for: pcdna

1. pcDNA5FRTTO-NHASt-codoptUL50

   Plasmid

   #135101

   Purpose

   Codon-optimized HSV-1 UL50 with N-terminal HAStrep tag

   Depositor

   Markus Landthaler

   Article

   Wyler et al Nat Commun. 2019 Oct 25;10(1):4878. doi:

   Insert

   HSV-1 UL50 codon optimized
   

   Use

   Tags

   HA-Strep-Strep

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Jan. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1(+)-FluoSTEP-EKAR

   Plasmid

   #181858

   Purpose

   Genetically encoded FRET-based sensor for monitoring ERK activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

   Depositor

   Jin Zhang

   Article

   Tenner et al Sci Adv. 2021 May 21;7(21). pii: 7/21/ea

   Insert

   FluoSTEP-EKAR
   

   Use

   Tags

   mRuby2 and sfGFP(1-10)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCDNA CrkII Y221F

   Plasmid

   #50732

   Purpose

   mammalian expression of CrkII Y221F mutant

   Depositor

   Kenneth Yamada

   Article

   Takino et al J Cell Sci. 2003 Aug 1;116(Pt 15):3145-5

   Insert

   CrkII Y221F (CRK Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Y221F

   Promoter

   CMV

   Available since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA-Flag-EcoCas6

   Plasmid

   #106274

   Purpose

   Encodes E.coli CRISPR Type I-E CasE with N-terminal fusion of Flag tag epitope and nuclear localization signal driven by CMV promoter

   Depositor

   Charles Gersbach

   Article

   Pickar-Oliver et al Nat Biotechnol. 2019 Sep 23. pii: 10.103

   Insert

   EcoCasE
   

   Use

   CRISPR

   Tags

   Flag, NLS

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 30, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-DKAR-Nuc

   Plasmid

   #45499

   Depositor

   Alexandra Newton

   Article

   Bossuyt et al J Biol Chem. 2011 Sep 23;286(38):33390-4

   Insert

   DKAR-Nuc
   

   Use

   Tags

   CFP, NLS, and YFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Sept. 26, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pcDNA3 HRPT2 136X

   Plasmid

   #11049

   Depositor

   Matthew Meyerson

   Article

   Rozenblatt-Rosen et al Mol Cell Biol. 2005 Jan . 25(2):612-20.

   Insert

   HRPT2 136X (CDC73 Human)
   

   Use

   Tags

   flag

   Expression

   Mammalian

   Mutation

   truncation mutant with a stop codon introduced at…

   Promoter

   Available since

   Dec. 6, 2005

   Availability

   Academic Institutions and Nonprofits only

7. pCDNA3.1-nAChr-beta3

   Plasmid

   #48811

   Purpose

   This report is the first to directly measure nAChR subunit stoichiometry using FRET and plasma membrane localization of α6- and β3-containing receptors using TIRF.

   Depositor

   Henry Lester, Jerry Stitzel

   Article

   Drenan et al Mol Pharmacol. 2008 Jan;73(1):27-41. Epu

   Insert

   nAChr Beta 3 subunit, isoform 1 (Chrnb3 Mouse)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   *

   Promoter

   CMV

   Available since

   Sept. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pcDNA5-FRT-TO-FH-Nsp7

   Plasmid

   #157689

   Purpose

   mammalian expression of N-terminally Flag-His8 tagged SARS-CoV-2 Nsp7 under control of a tetracycline-inducible promoter

   Depositor

   David Tollervey

   Article

   Bresson et al Wellcome Open Res. 2020 Nov 3;5:261. doi

   Insert

   FH-Nsp7 (ORF1ab SARS-CoV-2)
   

   Use

   Tags

   Flag-His8

   Expression

   Mammalian

   Mutation

   codon optimized

   Promoter

   Available since

   Aug. 5, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pcDNA3-gap16-ClopHensor

   Plasmid

   #26006

   Depositor

   Daniele Arosio

   Article

   Arosio et al Nat Methods. 2010 Jul . 7(7):516-8.

   Insert

   gap16-ClopHensor
   

   Use

   Tags

   Gap16 and Strep-II

   Expression

   Mammalian

   Mutation

   N-term (16AA) of Gap-43

   Promoter

   Available since

   Sept. 16, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA-p160(1-200)

    Plasmid

    #43

    Depositor

    Bruce Spiegelman

    Article

    Fan et al Genes Dev 2004 Feb 1;18(3):278-89. Epub

    Insert

    p160 myb binding protein (Mybbp1a Mouse)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only