We narrowed to 9,386 results for: BLI

1. pDGB3_alpha2_4xCBS-mDFR-Rep-RepA-tNOS (GB3625)

   Plasmid

   #225433

   Purpose

   Transcriptional unit for copper-regulation of BeYDV Rep/RepA with 4xCBS domain+minimal promoter DFR for induction by copper.

   Depositor

   Diego Orzaez

   Insert

   4xCBS:mDFR:Rep/RepA:Tnos
   

   Use

   Synthetic Biology

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   Oct. 3, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFRT-DestFLAGHA_HuB

   Plasmid

   #65758

   Purpose

   constitutively expresses FLAG-HA-tagged HuB in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Tags

   FLAG HA

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

3. GPX3

   Plasmid

   #38899

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   GPX3 (GPX3 Human)
   

   Tags

   Tev His Flag

   Expression

   Bacterial

   Available Since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

4. pFRT-TODestGFP_HuB

   Plasmid

   #65761

   Purpose

   expresses GFP-tagged HuB in mammalian cells

   Depositor

   Thomas Tuschl

   Insert

   ELAVL2 (ELAVL2 Human)
   

   Expression

   Mammalian

   Promoter

   CMV, 2x Tet

   Available Since

   June 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-UbC-eGFP-F

   Plasmid

   #71545

   Purpose

   Expresses a membrane bound eGFP, under the UbC promoter. This promoter expresses transgenes in neurons at higher levels than plasmids with the CMV or β-actin-CMV promoters.

   Depositor

   Pantelis Tsoulfas

   Insert

   eGFP-F
   

   Use

   AAV

   Tags

   contains 20- amino-acid farnesylation signal from…

   Expression

   Mammalian

   Promoter

   UbC

   Available Since

   Dec. 18, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. tol2-4xnr UAS-PA Rac1-mCherry

   Plasmid

   #41878

   Depositor

   Anna Huttenlocher

   Insert

   photoactivatable Rac1 (RAC1 Human)
   

   Use

   Zebrafish expression

   Tags

   6x His and mCherry

   Mutation

   Rac1 starts at I4 and contains mutations Q61L, E9…

   Available Since

   Oct. 30, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pNIC28-hKdm2b-CxxC-PHD

   Plasmid

   #49216

   Purpose

   Bacterial expression of human Kdm2b CxxC-PHD domain with N-His6-TEV and C-Avi tags. For use in BioCAP non-methylated DNA purification.

   Depositor

   Rob Klose

   Insert

   Kdm2b (KDM2B Human)
   

   Tags

   Avi and His6-TEV

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Dec. 18, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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8. COVID-SARS2 NSP16/10

   Plasmid

   #159615

   Purpose

   Bacterial co-expression vector fo Covid-SARS2 NSP16 and NSP10

   Depositor

   Opher Gileadi

   Inserts

   Covid-SARS2 Nsp16
   Covid-SARS2 Nsp10
   

   Tags

   His6-Zb-TEV and none

   Expression

   Bacterial

   Mutation

   Codon-optimized for E. coli expression

   Promoter

   (Bicistronic) and T7 - LacO

   Available Since

   Sept. 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. BASIC_SEVA_17_pKD46.10

   Plasmid

   #178917

   Purpose

   BASIC DNA assembly vector

   Depositor

   Paul Freemont

   Article

   Haines et al Synth Biol (Oxf). 2022 Oct 11;7(1):ysac0

   Insert

   mScarlet counter-selection cassette
   

   Use

   Synthetic Biology

   Available Since

   Feb. 2, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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10. pET21a-trAtCCD8(pYL735)

    Plasmid

    #178286

    Purpose

    Expresses trAtCCD8 in E. coli. pET21a carrying N-terminus 56 amino-acid truncated CCD8 from Arabidopsis

    Depositor

    Yanran Li

    Article

    Wu et al Sci Adv. 2021 Sep 17;7(38):eabh4048. doi

    Insert

    trAtCCD8
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Sept. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. Hs.NRAS Q61L

    Plasmid

    #83178

    Purpose

    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61L mutation

    Depositor

    Dominic Esposito

    Insert

    NRAS (NRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61L

    Available Since

    Sept. 14, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDest eGFP CUGBP1

    Plasmid

    #61276

    Purpose

    Mammalian expression of EGFP-tagged human CUGBP1

    Depositor

    Nicolas Charlet-Berguerand

    Insert

    CUG-BP (CELF1 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRT-DestFLAGHA_HuD

    Plasmid

    #65760

    Purpose

    constitutively expresses FLAG-HA-tagged HuD in mammalian cells

    Depositor

    Thomas Tuschl

    Insert

    ELAVL4 (ELAVL4 Human)
    

    Tags

    FLAG HA

    Expression

    Mammalian

    Promoter

    CMV, 2x Tet

    Available Since

    June 4, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pRN3P_TIR1

    Plasmid

    #119766

    Purpose

    in vitro transcription for mRNA

    Depositor

    Maria-Elena Torres-Padilla

    Article

    Borsos et al Nature. 2019 May;569(7758):729-733. doi:

    Insert

    TIR1
    

    Available Since

    March 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMT-HA-STX1

    Plasmid

    #89310

    Purpose

    Expresses HA tagged syntaxin 1 in Drosophila cells

    Depositor

    Andrew Peden

    Insert

    STX1 (Syx1A Fly)
    

    Tags

    HA tag

    Expression

    Insect

    Promoter

    P-MT (Metallothionein)

    Available Since

    Aug. 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pTXB1-CII

    Plasmid

    #51566

    Purpose

    Expression of bacteriophage lambda CII for protein purification (Intein-chiting binding domain fussion, New England Biolabs pTXB1 backbone

    Depositor

    Sankar Adhya, Francisco Malagon

    Insert

    cII
    

    Tags

    Intein-Chiting Binding Domanin

    Expression

    Bacterial

    Available Since

    March 25, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. pLEX307-APN-G418

    Plasmid

    #158456

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Sho Iketani, Alejandro Chavez

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pINDUCER20-FLAG-hPAX3 (#735)

    Plasmid

    #78336

    Purpose

    Doxycycline-inducible lentiviral overexpression of human PAX3 ORF with a FLAG tag

    Depositor

    Louis Kunkel, Matthew Alexander

    Insert

    PAX3 (PAX3 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Promoter

    TRE2

    Available Since

    July 12, 2016

    Availability

    Academic Institutions and Nonprofits only

19. Hs.HRAS Q61R

    Plasmid

    #83188

    Purpose

    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), Q61R mutation

    Depositor

    Dominic Esposito

    Insert

    HRAS (HRAS Human)
    

    Use

    Gateway entry clone

    Mutation

    Q61R

    Available Since

    Sept. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUG-amdSYM-mak10TR

    Plasmid

    #75433

    Purpose

    Generates truncated version of MAK10 gene, which is required for dsRNA L-A propagation in S.cerevisiae

    Depositor

    John McCusker

    Inserts

    MAK10TR
    ApR
    amdSYM
    

    Expression

    Yeast

    Mutation

    N-terminal 740 bp of MAK10 ORF

    Promoter

    Native promoter and PTEF

    Available Since

    Jan. 22, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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