We narrowed to 8,358 results for: BLI

1. pAAV hSyn1 bPAC cMyc S27A 2A tDimer

   Plasmid

   #85398

   Purpose

   humanized photoactivated adenylyl cyclase lower dark activity, spectrum shifted 15 nm longer wavelenght with neuron-specific promoter, plus red fluorescent protein

   Depositor

   Thomas Oertner

   Inserts

   humanized photoactivated adenylyl cyclase
   red fluorescent protein
   

   Use

   AAV

   Tags

   cMyc Tag

   Expression

   Mammalian

   Mutation

   S27A

   Promoter

   human Synapsin1 promotor and ribosomal skip seque…

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pDONR221_SMAD2_HAe2_Bsd

   Plasmid

   #167166

   Purpose

   Blasticidin selection cassette flanked by homology arms from human SMAD2 exon 2 for use in creating CRISPR-Cas9 mediated gene knockout.

   Depositor

   Alexander Loewer

   Insert

   Repair template to knockout SMAD2 by inserting a blasticidin resistance cassette (SMAD2 Human)
   

   Use

   Repair template for gene editing in human cells

   Tags

   blasticidin

   Expression

   Mutation

   Promoter

   Available since

   May 28, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCS2+5'MT-wbp2nl

   Plasmid

   #172467

   Purpose

   expresses myc-tagged version of wild-type protein

   Depositor

   Sally Moody

   Article

   Marchak et al Dev Biol. 2017 Sep 1;429(1):213-224. doi

   Insert

   wbp2nl
   

   Use

   Xenopus expression

   Tags

   Myc

   Expression

   Mutation

   Promoter

   SP6

   Available since

   Oct. 7, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Hs.KRAS4a G13D

   Plasmid

   #83170

   Purpose

   Gateway ORF Entry clone of human KRAS4A [NM_033360.3] with stop codon (for native or N-terminal fusions), G13D mutation

   Depositor

   Dominic Esposito

   Insert

   KRAS (KRAS Human)
   

   Use

   Gateway entry clone

   Tags

   Expression

   Mutation

   G13D

   Promoter

   Available since

   Sept. 14, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. MAP3K3

   Plasmid

   #38937

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Nicola Burgess-Brown

   Insert

   MAP3K3 (MAPK3 Human)
   

   Use

   Tags

   His Tev

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   March 18, 2013

   Availability

   Academic Institutions and Nonprofits only

6. SDC229-B03

   Plasmid

   #58763

   Purpose

   bacterial expression of PDB 4PYZ

   Depositor

   Cheryl Arrowsmith

   Insert

   PDB:4PYZ (USP7 Human)
   

   Use

   Tags

   His

   Expression

   Bacterial

   Mutation

   Bacterial expression for structure determination;…

   Promoter

   T7

   Available since

   Aug. 5, 2014

   Availability

   Industry, Academic Institutions, and Nonprofits

7. ataxin3:1-431_Q79_BV

   Plasmid

   #209733

   Purpose

   Bacterial expression for structure determination

   Depositor

   Cheryl Arrowsmith

   Insert

   Ataxin 3 (ATXN3 Human)
   

   Use

   Tags

   6xHis-SBP-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   Polyhedrin

   Available since

   Nov. 13, 2023

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pLKO-Tet-On-HDAC1-shRNA3

   Plasmid

   #90243

   Purpose

   Lentivirus for expression of shRNA3 against human HDAC1 (Dox-inducible)

   Depositor

   Roland Friedel

   Insert

   HDAC1 (HDAC1 Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available since

   May 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. Myog'-2A-mCherryNLS-PGK-Puro

   Plasmid

   #69548

   Purpose

   Donor vector for homologous recombination to insert a 2A-mCherry-NLS cassette in place of the stop codon of myogenin in the mouse genome.

   Depositor

   Charles Gersbach

   Inserts

   2A-mCherry-NLS
   Myogenin 5' homology arm
   Myogenin 3' homology arm (Myog Mouse)
   

   Use

   Mouse Targeting

   Tags

   2x nuclear localization signal and PVT1-2A "…

   Expression

   Mutation

   Silent V205V mutation in myogenin gene to prevent…

   Promoter

   Available since

   April 12, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. human KANK1 Ankyrin Repeats pET151

    Plasmid

    #164053

    Purpose

    Expresses human KANK1 Ank domain in bacterial cells

    Depositor

    Ben Goult

    Insert

    Kidney Ankyrin Repeat-Containing Protein 1 (KANK1 Human)
    

    Use

    Tags

    His-tag, TEV cleavage site

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Oct. 6, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. MS2_SC2_S_Omicron

    Plasmid

    #180229

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WHO Omicron SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Omicron (Amino Acid 319-869)

    Promoter

    T7

    Available since

    Jan. 28, 2022

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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12. MS2_SC2_S_WT

    Plasmid

    #175044

    Purpose

    Generation of MS2 Virus-Like Particles packaged with the sequence for the WT SARS-CoV-2 S gene (Amino Acid 319-869)

    Depositor

    Paul Freemont

    Inserts

    Maturation Protein
    Coat Protein Dimer
    S gene (S SARS-CoV-2)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    WT (Amino Acid 319-869)

    Promoter

    T7

    Available since

    Oct. 18, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. AKT3

    Plasmid

    #39038

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Nicola Burgess-Brown

    Insert

    AKT3 (AKT3 Human)
    

    Use

    Tags

    His Tev

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 18, 2013

    Availability

    Academic Institutions and Nonprofits only

14. pQTEV-PFDN2

    Plasmid

    #31329

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    prefoldin 2 (PFDN2 Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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15. Syn-ChR-tdTomato

    Plasmid

    #99843

    Purpose

    AAV expression of ChR2(H134R)-tdTomato under the Syn promoter

    Depositor

    Gordon Shepherd

    Insert

    ChR2(H134R)-tdTomato
    

    Use

    AAV

    Tags

    tdTomato

    Expression

    Mammalian

    Mutation

    ChR2(H134R), carrying a single point mutation at …

    Promoter

    human synapsin I promoter

    Available since

    Jan. 26, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. human EB1-GFP mutagenized S16 R17 H18 D19 W23 N25 all converted to A (JB139)

    Plasmid

    #39300

    Depositor

    Jennifer Tirnauer, Tim Mitchison

    Insert

    EB1 (MAPRE1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    S16A, R17A, H18A, D19A, W23A and N25A

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

17. CYP7A1 (3SN5)

    Plasmid

    #32874

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    CYP7A1 (CYP7A1 Human)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    amino acids 25-503 only

    Promoter

    Available since

    Jan. 13, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

18. WDR5

    Plasmid

    #36900

    Purpose

    Bacterial expression for structure determination; may not be full ORF

    Depositor

    Cheryl Arrowsmith

    Insert

    WDR5 (WDR5 Human)
    

    Use

    Tags

    His tag with TEV cleavage

    Expression

    Bacterial

    Mutation

    mutation version of plasmid #60121 3UR4

    Promoter

    Available since

    Aug. 8, 2012

    Availability

    Industry, Academic Institutions, and Nonprofits

19. pQTEV-MTPN

    Plasmid

    #34829

    Depositor

    Konrad Buessow

    Article

    Bussow et al Microb Cell Fact. 2005 Jul 5. 4():21.

    Insert

    myotrophin (MTPN Human)
    

    Use

    Tags

    His and TEV

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    March 22, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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20. pUASp YFP Rac1 CA

    Plasmid

    #52235

    Purpose

    Drosophila expression of YFP-Rac1 constitutively active mutant

    Depositor

    Matthew Scott

    Insert

    Rac1 (Rac1 Fly)
    

    Use

    Drosophila

    Tags

    YFP

    Expression

    Insect

    Mutation

    Q61L; CA mutation

    Promoter

    GAL4

    Available since

    March 21, 2014

    Availability

    Academic Institutions and Nonprofits only