We narrowed to 10,337 results for: plasmids 101

1. pAAV-EF1a-double floxed-Gad67-WPRE-HGHpA

   Plasmid

   #184632

   Purpose

   Expresses Gad67, driven by the Ef1a promoter, in a Cre-dependent fashion

   Depositor

   Nicolas Tritsch

   Article

   Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

   Insert

   Gad67 (Gad1 Mouse)
   

   Use

   AAV

   Tags

   Expression

   Mutation

   Promoter

   EF1a

   Available Since

   Aug. 9, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pOTTC407 - pAAV EF1a eGFP

   Plasmid

   #60058

   Purpose

   An AAV packaging vector that expresses enhanced green fluorescent protein under control of the EF1a promoter.

   Depositor

   Brandon Harvey

   Article

   Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

   Insert

   enhanced Green Fluorescent Protein
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available Since

   Oct. 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (HNF1A)

   Plasmid

   #188384

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3.1-hRXRα

   Plasmid

   #135910

   Purpose

   Human Retinoid X Receptor-alpha in pcDNA3.1(+) plasmid expression vector using SV40 Promoter

   Depositor

   Catharine Ross

   Article

   Zolfaghari et al Anal Biochem. 2019 Jul 15;577:98-109. do

   Insert

   Human retinoid X receptor- alpha (RXRA Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available Since

   Feb. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pHR-scFv-GCN4-sfGFP-GB1-NLS-iLID-dWPRE

   Plasmid

   #121966

   Purpose

   Expresses fusion of single chain variable fragment recognizing SunTag, fluorescent protein sfGFP, and iLID which upon light activation binds to sspB.

   Depositor

   Clifford Brangwynne

   Article

   Shin et al Cell. 2018 Nov 29;175(6):1481-1491.e13.

   Insert

   scFv-GCN4
   

   Use

   Lentiviral

   Tags

   superfold GFP-GB1-NLS-iLID

   Expression

   Mammalian

   Mutation

   Promoter

   SFFV

   Available Since

   Feb. 21, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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6. pMSCV-NLS-CBGreen-FIRE-puro

   Plasmid

   #139195

   Purpose

   Luminescent reporter of ERK activity

   Depositor

   Matthew Lazzara

   Article

   Day et al Cell Rep. 2020 Mar 10;30(10):3383-3396.e

   Insert

   CBG99
   

   Use

   Luciferase and Retroviral

   Tags

   Fra1 PEST domain fragment (FIRE) and nuclear loca…

   Expression

   Mammalian

   Mutation

   See depositor comments below

   Promoter

   Available Since

   Sept. 9, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHR_PGK_SNIPR_Hinge Notch SNIPR (HNF1A, ALPPL2 scFv)

   Plasmid

   #188385

   Purpose

   Lentiviral vector - constitutive expression of an anti-ALPPL2 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a HNF1A(DBD)-p65(361-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiALPPL2_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_HNF1A(DBD)-p65(361-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pG3H-PE3max-attR1R2

   Plasmid

   #213049

   Purpose

   Destination vector Expressing nCas9 and RT fusion protein and carrying Gateway recombination sites

   Depositor

   Bing Yang

   Article

   Gupta et al Plant Commun. 2023 Oct 26:100741. doi: 1

   Inserts

   Cas9 nickase
   M-MLV Reverse Transcriptase
   

   Use

   Note: this plasmid needs helper pvs1-vir2 for rep…

   Tags

   Expression

   Plant

   Mutation

   H840A, R240K, N413K,

   Promoter

   Available Since

   Feb. 8, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pHR_PGK_SNIPR_Humanized Hinge Notch SNIPR (Pax6)

   Plasmid

   #188383

   Purpose

   Lentiviral vector - constitutive expression of an anti-CD19 SNIPR with a CD8alpha-variant2-ECD, a Notch1-TMD, a Notch2-JMD, and a Pax6(DBD)-p65(428-551) transcriptional factor

   Depositor

   Kole Roybal

   Article

   Zhu et al Cell. 2022 Apr 14;185(8):1431-1443.e16.

   Insert

   PGK_antiCD19_CD8alpha-variant2-ECD_Notch1-TMD_Notch2-JMD_Pax6(DBD)-p65(428-551)
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   March 9, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAV-EF1a-double floxed-Gat1-HA-WPRE-HGHpA

    Plasmid

    #184635

    Purpose

    Expresses the GABA membrane transporter Gat1 fused to HA, driven by the Ef1a promoter, in a Cre-dependent fashion

    Depositor

    Nicolas Tritsch

    Article

    Melani et al Cell Rep. 2022 Apr 19;39(3):110716. doi:

    Insert

    Gat1 (Slc6a1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available Since

    Aug. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. mCherry-DRP1 K38A

    Plasmid

    #232350

    Purpose

    Expresses K38A variant of human DRP1 with mCherry tag

    Depositor

    Samantha Lewis

    Article

    Begeman et al bioRxiv [Preprint]. 2024 Aug 17:2024.08.

    Insert

    DRP1 (DNM1L Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    K38A

    Promoter

    CMV

    Available Since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. CLYBL-TO-PAX7

    Plasmid

    #204346

    Purpose

    Knock-in vector to insert a dox-inducible PAX7 transgene into the CYBL locus in human pluripotent stem cells. PAX7 induction results in myogenic differentiation.

    Depositor

    Ivo Lieberam

    Article

    Harley et al Cell Rep. 2023 Dec 26;42(12):113509. doi

    Insert

    human PAX7
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    TRE3G

    Available Since

    Oct. 17, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMGS46 (ARF16-PB1-HA-P2A-OsTIR1)

    Plasmid

    #126580

    Purpose

    A repair construct to express ARF16-PB1-HA and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. (565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP

    Plasmid

    #163030

    Purpose

    Expression of CRISPRi with gSTOP gRNA

    Depositor

    Tonia Rex

    Article

    Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

    Insert

    Mammalian codon-optimized SadCas9
    

    Use

    AAV

    Tags

    KRAB, SV40 pA, and myc NLS

    Expression

    Mammalian

    Mutation

    Promoter

    Alb-AAT

    Available Since

    Jan. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pGL4_10xUAS-CMVmin-BC0396-luc2

    Plasmid

    #227115

    Purpose

    Barcoded assay, UAS sensor for split TEV assays; barcode BC0396

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    10x clustered UAS element linked to CMV minimal promoter driving barcode 0396 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Dec. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pMGS56 (GFP-ARF16-PB1-P2A-OsTIR1)

    Plasmid

    #129668

    Purpose

    A repair construct to express GFP-ARF16-PB1 and OsTIR1 under the control of the CMV promoter from the human AAVS1 locus

    Depositor

    Michael Guertin

    Article

    Sathyan et al Genes Dev. 2019 Oct 1;33(19-20):1441-145

    Insert

    OsARF16-PB1 domain
    

    Use

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pGL4_NFAT-RE-CMVmin-BC0538-luc2

    Plasmid

    #227140

    Purpose

    Barcoded assay, Ca2+ sensor; barcode BC05038

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered NFAT element linked to CMV minimal promoter driving barcode 05038 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Nov. 26, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pGL4_CRE-CMVmin-BC0491-luc2

    Plasmid

    #227133

    Purpose

    Barcoded assay, cAMP/Ca2+ sensor; barcode BC0491

    Depositor

    Michael Wehr

    Article

    Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

    Insert

    6x clustered CRE element linked to CMV minimal promoter driving barcode 0491 and a luciferase reporter gene
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Jan. 31, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLV-EF1a-AcGFP-P2A-hBRD2

    Plasmid

    #197880

    Purpose

    study roles of human BET proteins in human pluripotency reprogramming

    Depositor

    Kejin Hu

    Article

    Hossain et al iScience. 2022 Dec 28;26(1):105889. doi:

    Insert

    BRD2 (BRD2 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 22, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. 1000.pCCLsin.cPPT.hPGK.Ascl1.WPRE

    Plasmid

    #67291

    Purpose

    Induce human iN conversion from fibroblast

    Depositor

    Malin Parmar

    Article

    Lau et al Cell Rep. 2014 Dec 11;9(5):1673-80. doi:

    Insert

    Asc1 (Ascl1 Mouse)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    PGK

    Available Since

    Aug. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge