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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 9,386 results for: BLI

Showing: 6421 - 6440 of 9386 results

  1. Hs.HRAS G12D

    Plasmid
    #83183
    Purpose
    Gateway ORF Entry clone of human HRAS [NM_005343.2 ] with stop codon (for native or N-terminal fusions), G12D mutation
    Depositor
    Dominic Esposito
    Insert
    HRAS (HRAS Human)
    Use
    Gateway entry clone
    Mutation
    G12D
    Available Since
    Sept. 15, 2016
    Availability
    Academic Institutions and Nonprofits only
    83183-plasmid-map-sequence-id-158146

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  2. Hs.NRAS Q61K

    Plasmid
    #83180
    Purpose
    Gateway ORF Entry clone of human NRAS [NM_002524.4 ] with stop codon (for native or N-terminal fusions), Q61K mutation
    Depositor
    Dominic Esposito
    Insert
    NRAS (NRAS Human)
    Use
    Gateway entry clone
    Mutation
    Q61K
    Available Since
    Sept. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    83180-plasmid-map-sequence-id-158143

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  3. NEK2

    Plasmid
    #39163
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    NEK2 (NEK2 Human)
    Tags
    His6
    Expression
    Bacterial
    Mutation
    T175A inactivating mutant
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  4. pDONR221-CTSB

    Plasmid
    #158443
    Purpose
    Gateway Cloning compatible entry vector for the human CTSB gene.
    Depositor
    Sho Iketani, Alejandro Chavez
    Insert
    CTSB (CTSB Human)
    Use
    Gateway entry vector
    Available Since
    Aug. 19, 2020
    Availability
    Academic Institutions and Nonprofits only
    158443-plasmid-map-sequence-id-308885

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  5. 4H4H

    Plasmid
    #51314
    Depositor
    Cheryl Arrowsmith
    Insert
    4H4H (EHMT1 Human, 4H4H)
    Tags
    His
    Expression
    Bacterial
    Mutation
    Truncation: See Comments
    Promoter
    T7
    Available Since
    Feb. 12, 2014
    Availability
    Industry, Academic Institutions, and Nonprofits

  6. pAAV-DIO-EF1a-TVA-P2A-EYFP

    Plasmid
    #177017
    Purpose
    For expression of the avian TVA receptor in a cre-dependent manner
    Depositor
    Peter Jonas
    Insert
    TVA-P2A-EYFP (CD320 Coturnix japonica)
    Use
    AAV and Cre/Lox
    Tags
    EYFP
    Expression
    Mammalian
    Promoter
    CAGGS
    Available Since
    Feb. 4, 2022
    Availability
    Academic Institutions and Nonprofits only
    177017-plasmid-map-sequence-id-353740

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  7. pLenti-cytoMetGFP

    Plasmid
    #37561
    Depositor
    David Rimm
    Insert
    MET (MET Human)
    Use
    Lentiviral
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    only contains cytoplasmic domain amino acids K956…
    Promoter
    CMV
    Available Since
    Aug. 7, 2012
    Availability
    Academic Institutions and Nonprofits only
    37561-plasmid-map-sequence-id-47323

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  8. hSRF-3xHA-IRES-hrGFP (#479)

    Plasmid
    #78347
    Purpose
    Overexpression of human SRF ORF with a 3xHA C-terminal tag and an IRES-hrGFP reporter
    Depositor
    Louis Kunkel, Matthew Alexander
    Insert
    SRF (SRF Human)
    Tags
    3x HA tag and IRES-hrGFP
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    July 29, 2016
    Availability
    Academic Institutions and Nonprofits only

  9. pMT-TAK1-V5-His

    Plasmid
    #63753
    Purpose
    Expresses drosophila TAK1 tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
    Depositor
    Cyril Gueydan
    Insert
    TGF-β activated kinase 1 (Tak1 Fly)
    Tags
    V5-His
    Expression
    Insect
    Promoter
    pMT
    Available Since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only
    63753-plasmid-map-sequence-id-106438

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  10. pAAV Syn 2xLyn-ERex-mKate2-bPAC(F198Y)

    Plasmid
    #219654
    Purpose
    Neuronal expression of PACmn with mKate2, a membrane targeted version of the photoactivatable adenylyl cyclase from Beggiatoa with lowered dark activity.
    Depositor
    Thomas Oertner
    Insert
    2xLyn-ERex-mKate2-bPAC(F198Y)
    Use
    AAV
    Tags
    ER exit ( FCYENE) and Lyn11 (GCIKSKGKDS)
    Expression
    Mammalian
    Promoter
    Syn
    Available Since
    May 28, 2024
    Availability
    Academic Institutions and Nonprofits only
    219654-plasmid-map-sequence-id-434848

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  11. ACE2 g3

    Plasmid
    #153013
    Purpose
    A guide RNA targeting ACE2 in a lentiviral plasmid co-expressing mCherry
    Depositor
    Jason Sheltzer
    Insert
    ACE2 (ACE2 Human)
    Use
    CRISPR and Lentiviral
    Tags
    mCherry
    Available Since
    June 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    153013-plasmid-map-sequence-id-298679

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  12. MARS

    Plasmid
    #205232
    Purpose
    Expresses PLEKHA5 aa 143-271 (K163A and R164A) fused to mScarlet-i in mammalian cells
    Depositor
    Jeremy Baskin
    Insert
    PLEKHA5 aa 143-271 with K163A and R164A mutations (PLEKHA5 Human)
    Tags
    Nuclear Export Sequence and mScarlet-i
    Expression
    Mammalian
    Mutation
    amino acids 143-271 of PLEKHA5 (GenBank reference…
    Promoter
    CMV
    Available Since
    July 30, 2024
    Availability
    Academic Institutions and Nonprofits only
    205232-plasmid-map-sequence-id-404748

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  13. K560GFP_17NL

    Plasmid
    #129767
    Purpose
    Expresses Drosophila Kinesin-1 with extended neck linker (DAL insert) truncated at 560 and GFP tagged for motility assays
    Depositor
    William Hancock
    Insert
    KHC (Khc Fly)
    Tags
    GFP and His6
    Expression
    Bacterial
    Mutation
    DAL insert before A345
    Available Since
    Nov. 20, 2019
    Availability
    Academic Institutions and Nonprofits only
    129767-plasmid-map-sequence-id-263589

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  14. pLenti-L3US2-RFP-HDAC3

    Plasmid
    #83966
    Purpose
    Lentiviral CRISPR HDAC3 dual gRNA targeting vector
    Depositor
    Eleanor Chen
    Insert
    HDAC3 dual gRNAs (HDAC3 Human)
    Use
    Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Dec. 6, 2016
    Availability
    Academic Institutions and Nonprofits only
    83966-plasmid-map-sequence-id-160487

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  15. pES133

    Plasmid
    #127552
    Purpose
    Variant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13
    Depositor
    Gavin Thomas, Emmanuele Severi
    Insert
    F5-flanked tetracycline resistance cassette
    Expression
    Bacterial
    Mutation
    Multiple SNPs in tetA (originally from pBR322) to…
    Available Since
    July 30, 2019
    Availability
    Academic Institutions and Nonprofits only
    127552-plasmid-map-sequence-id-251926

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  16. pAG416-GAL-hnRNPA1

    Plasmid
    #181710
    Purpose
    Galactose inducible expression of hnRNPA1
    Depositor
    Alejandro Chavez
    Insert
    hnRNPA1 (HNRNPA1 Human)
    Expression
    Yeast
    Available Since
    April 24, 2024
    Availability
    Academic Institutions and Nonprofits only
    181710-plasmid-map-sequence-id-430953

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  17. tet-pLKO.puro_shHuR CDS

    Plasmid
    #110411
    Purpose
    TRCN0000276129 (Target CGAGCTCAGAGGTGATCAAAG), silence human ELAVL1 (HuR) gene, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    ELAVL1 (HuR)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    Feb. 23, 2021
    Availability
    Academic Institutions and Nonprofits only
    110411-plasmid-map-sequence-id-213311

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  18. HPGD

    Plasmid
    #39154
    Purpose
    Bacterial expression for structure determination; may not be full ORF
    Depositor
    Nicola Burgess-Brown
    Insert
    HPGD (HPGD Human)
    Tags
    His Tev
    Expression
    Bacterial
    Mutation
    codon-optimized
    Available Since
    March 18, 2013
    Availability
    Academic Institutions and Nonprofits only

  19. tet-pLKO.puro_shGAC

    Plasmid
    #110423
    Purpose
    shGAC, silence glutaminase GAC isoform, doxycycline inducible, puromycin selection
    Depositor
    Sandra Martha Gomes Dias
    Insert
    GLS glutaminase (GLS Human)
    Use
    Lentiviral and RNAi
    Expression
    Mammalian
    Promoter
    H1/TO (RNA PolIII)
    Available Since
    June 19, 2018
    Availability
    Academic Institutions and Nonprofits only
    110423-plasmid-map-sequence-id-213314

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  20. isoform PKC Iota (JCE597)

    Plasmid
    #182030
    Purpose
    yeast expression of mouse PKC Iota with yeast PKC1 promoter and ADH1 terminator
    Depositor
    Juan Carlos Igual
    Insert
    PKC Iota (Prkci Mouse)
    Expression
    Mammalian
    Promoter
    yeast PKC1 promoter (-600 to -1)
    Available Since
    May 17, 2022
    Availability
    Academic Institutions and Nonprofits only
    182030-plasmid-map-sequence-id-361960

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Showing: 6421 - 6440 of 9386 results