We narrowed to 15,682 results for: grna

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-1

Plasmid

#128756

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-sgRNA EF1Alpha-puro-T2A-BFP.PAPD4-2

Plasmid

#128757

Purpose

Expresses PAPD4 gRNA for CRISPRi

Depositor

David Bartel

Article

Kleaveland et al Cell. 2018 Jul 12;174(2):350-362.e17. do

Insert

PAPD4 sgRNA #1 (TENT2 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

mU6

Available Since

Aug. 14, 2019

Availability

Academic Institutions and Nonprofits only

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B270 (plasmid expressing ATP1A1 sgSTOP and containing an empty sgRNA-expression cassette)

Plasmid

#100716

Purpose

B52 plasmid expressing ATP1A1 sgSTOP (cloned in BsmBI site) and containing an empty sgRNA-expression cassette (use BbsI for cloning)

Depositor

Alberto Ciccia

Article

Billon et al Mol Cell. 2017 Sep 4. pii: S1097-2765(17

Insert

sgSTOP targeting ATP1A1 (cloned using BsmBI)

Use

CRISPR

Expression

Mammalian

Promoter

U6 promoters

Available Since

Sept. 19, 2017

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Dicer_1

Plasmid

#68807

Purpose

specific sgRNA against mouse Dicer1 gene cloned in the pX330 backbone (Addgene Number 42230). Generation of Dicer1 knockout mESCs.

Depositor

Constance Ciaudo

Article

Bodak et al FEBS Open Bio. 2017 Jan 11;7(2):204-220.

Insert

sgRNA mouse Dicer1

Use

CRISPR and Mouse Targeting

Expression

Bacterial and Mammalian

Available Since

Feb. 13, 2017

Availability

Academic Institutions and Nonprofits only

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C8orf44-SGK3 gRNA (BRDN0001147093)

Plasmid

#75907

Purpose

3rd generation lentiviral gRNA plasmid targeting human C8orf44-SGK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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C8orf44-SGK3 gRNA (BRDN0001148559)

Plasmid

#75908

Purpose

3rd generation lentiviral gRNA plasmid targeting human C8orf44-SGK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NME1-NME2 gRNA (BRDN0001162367)

Plasmid

#75888

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1-NME2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001145543)

Plasmid

#75902

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001147862)

Plasmid

#75903

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001147836)

Plasmid

#75904

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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FPGT-TNNI3K gRNA (BRDN0001148036)

Plasmid

#75905

Purpose

3rd generation lentiviral gRNA plasmid targeting human FPGT-TNNI3K

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

FPGT-TNNI3K,TNNI3K (FPGT-TNNI3K Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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C8orf44-SGK3 gRNA (BRDN0001146567)

Plasmid

#75906

Purpose

3rd generation lentiviral gRNA plasmid targeting human C8orf44-SGK3

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

C8orf44-SGK3,SGK3 (SGK3 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NME1-NME2 gRNA (BRDN0001145211)

Plasmid

#75885

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1-NME2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NME1-NME2 gRNA (BRDN0001147480)

Plasmid

#75886

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1-NME2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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NME1-NME2 gRNA (BRDN0001162403)

Plasmid

#75887

Purpose

3rd generation lentiviral gRNA plasmid targeting human NME1-NME2

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

NME1,NME1-NME2 (NME1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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AiO-Cas12a: All in one enAsCas12a expression and gRNA cloning backbone

Plasmid

#228364

Purpose

mU6-driven expression of enAsCas12a gRNAs with EF1a-enAsCas12a-2A-Puro

Depositor

Michael Boettcher

Article

El Kassem et al Nat Commun. 2025 Feb 14;16(1):1631. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral; Enascas12a nuclease and en…

Expression

Mammalian

Promoter

mU6

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pSpCas9(BB)-2A-Puro (PX459)+gRNA miR-124-1/2-5'

Plasmid

#117322

Purpose

CRISPR-Cas9 for miR-124-1/2, 5'

Depositor

Volker Busskamp

Article

Kutsche et al Cell Syst. 2018 Sep 28. pii: S2405-4712(

Insert

gRNA miR-124-1/2-5' (MIR124-1 Synthetic)

Tags

3xFLAG

Expression

Mammalian

Promoter

U6

Available Since

Dec. 5, 2018

Availability

Academic Institutions and Nonprofits only

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p(U6b-BsaI-gRNA)

Plasmid

#65956

Purpose

Tribolium U6b promoter with BsaI cloning site to insert guide RNA sequence

Depositor

Michalis Averof

Article

Gilles et al Development. 2015 Jul 9. pii: dev.125054

Type

Empty backbone

Expression

Insect

Promoter

U6

Available Since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-SDHB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172670

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human SDHB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

SDHB pegRNA and SDHB_nick-sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Aug. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCfB3053(gRNA X-2, XI-5, XII-4)

Plasmid

#73295

Purpose

EasyClone-MarkerFree guiding RNA vector to direct Cas9 to cut at sites X-2, XI-5, and XII-4

Depositor

Irina Borodina

Article

Jessop-Fabre et al Biotechnol J. 2016 Aug;11(8):1110-7. doi

Insert

guiding RNA

Use

CRISPR; Grna

Expression

Yeast

Available Since

Nov. 23, 2016

Availability

Academic Institutions and Nonprofits only

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