We narrowed to 12,355 results for: nsf

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pTRANS_263

Plasmid

#91127

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:bar, Viral Replication: WDV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

35S

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_102

Plasmid

#91200

Purpose

Transformation, Type: non-T-DNA, Plant Selection: none, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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Sa-SchCas9

Plasmid

#192137

Purpose

Expresses Sa-SchCas9, and cloning backbone for sgRNA

Depositor

Yongming Wang

Article

Wang et al Adv Sci (Weinh). 2022 Feb;9(4):e2104789.

Insert

Sa-SchCas9

Use

AAV

Expression

Mammalian

Available Since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDTnLAPP2A frame 0

Plasmid

#28226

Depositor

Harald von Melchner

Article

Schnutgen et al Nat Methods. 2011 Feb . 8(2):103-4.

Insert

Hygrophosphotransferase-P2A-Enhanced Green Fluorescent Protein

Use

Retroviral

Tags

EGFP and nLAP-tag

Available Since

Dec. 7, 2011

Availability

Academic Institutions and Nonprofits only

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pET28a-GST-Q11

Plasmid

#185790

Purpose

Encodes glutathione S-transferase linked to Q11 self-assembling peptide via a GS-linker sequence

Depositor

Lizhong He

Article

Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

Insert

GST-Q11

Tags

His-tag

Expression

Bacterial

Promoter

T7

Available Since

July 22, 2022

Availability

Academic Institutions and Nonprofits only

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pHCKan-yibDp-GST

Plasmid

#134592

Purpose

Low Phosphate inducible GST

Depositor

Michael Lynch

Article

Menacho-Melgar et al Biotechnol Bioeng. 2020 May 22. doi: 10.

Insert

Glutathione S-transferase

Tags

none

Expression

Bacterial

Promoter

yibD

Available Since

May 5, 2020

Availability

Academic Institutions and Nonprofits only

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pTH848-gst-disec

Plasmid

#89589

Purpose

Plasmid for bacterial expression of His-tagged GST encoded by non-preferred codons

Depositor

Tobias von der Haar

Article

Jossé et al bioRxiv 200006

Insert

glutathione-S transferase codon-dis-optimised for bacterial expression

Tags

8xhistidine

Expression

Bacterial

Promoter

T7

Available Since

March 20, 2018

Availability

Academic Institutions and Nonprofits only

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pICH42222

Plasmid

#50328

Depositor

Sylvestre Marillonnet, Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

CDS, phosphinothricin acetyl transferase, (S. hygroscopicus)

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation; See De…

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pICSL70005

Plasmid

#50335

Depositor

Nicola Patron

Article

Engler et al ACS Synth Biol. 2014 Nov 21;3(11):839-43

Insert

bialaphos resistance cassette (Nos promoter (A. tumefaciens) + phosphinothricin acetyl transferase (S. hygroscopicus) + Nos term

Use

Plant expression

Mutation

BsaI/BbsI sites removed by point-mutation

Available Since

Feb. 18, 2014

Availability

Academic Institutions and Nonprofits only

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pAAV-FLEX-SaCas9-U6-sgKcnab2

Plasmid

#192795

Purpose

Encodes SaCas9 and an sgRNA targeting mouse Kcnab2

Depositor

Marta Soden

Article

Yee et al J Neurophysiol. 2022 Jul 1;128(1):62-72.

Insert

sgKcnab2

Use

AAV and CRISPR

Promoter

U6

Available Since

Dec. 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV syn CCL20-pre-mGRASP

Plasmid

#173117

Purpose

Expresses CCL20 sender construct in neurons

Depositor

Alice Ting

Article

Cho et al Elife. 2022 Mar 21;11:e70881. doi: 10.75

Insert

CCL20-pre-mGRASP

Use

AAV

Promoter

Synapsin

Available Since

Dec. 21, 2022

Availability

Academic Institutions and Nonprofits only

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pAAVf-EnhCB-lacZnls

Plasmid

#35642

Depositor

Phillip Zamore

Article

Xie et al Nat Methods. 2012 Mar 4;9(4):403-9. doi:

Type

Empty backbone

Use

AAV

Available Since

April 25, 2012

Availability

Academic Institutions and Nonprofits only

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pTRANS_110

Plasmid

#91202

Purpose

Transformation, Type: non-T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

April 23, 2018

Availability

Academic Institutions and Nonprofits only

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pDHt-KS

Plasmid

#63616

Purpose

The multiple cloning site of pGreenII0000 cloned into binary vector pDHt

Depositor

Seogchan Kang

Article

Khang et al Fungal Genet Biol. 2005 Jun;42(6):483-92

Type

Empty backbone

Use

Fungal expression

Available Since

July 27, 2015

Availability

Academic Institutions and Nonprofits only

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pAAV-CMV-FLEX-SaCas9-U6-sgKcnj6

Plasmid

#159913

Purpose

Mutagenesis of Kcnj6

Depositor

Larry Zweifel

Insert

Kcnj6 (Kcnj6 Mouse)

Use

AAV, CRISPR, and Mouse Targeting

Available Since

Nov. 16, 2020

Availability

Academic Institutions and Nonprofits only

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MTK3_004

Plasmid

#123720

Purpose

Encodes hph (hygromycin B phosphotransferase) as a Type 3 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

Hygro Resistance

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pTRANS_130

Plasmid

#91204

Purpose

Transformation, Type: non-T-DNA, Plant Selection: 2x35S:bar, Viral Replication: none

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pGEXndoE(E662Q)

Plasmid

#47716

Purpose

Expresses GST-EndoE(E662Q) from E. faecalis in E. coli

Depositor

Mattias Collin, Vincent Fischetti

Article

Collin et al J Biol Chem. 2004 May 21;279(21):22558-7

Insert

ndoE

Tags

GST-EndoE(E186Q) and Glutathione S-transferase

Expression

Bacterial

Mutation

Changed Glu-662 to Gln

Promoter

tac

Available Since

Jan. 27, 2016

Availability

Academic Institutions and Nonprofits only

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pTRANS_202

Plasmid

#91106

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Expression

Plant

Promoter

No Promoter

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA EMX1

Plasmid

#128121

Purpose

Co-expresses sgRNA EMX1, SpyCas9 scaffold (F+E) and GFP (transfection marker)

Depositor

Dominik Niopek

Article

Aschenbrenner et al Sci Adv. 2020 Feb 5;6(6):eaay0187. doi:

Insert

sgRNA EMX1 + GFP

Use

CRISPR and Synthetic Biology

Expression

Mammalian

Promoter

RSV/U6

Available Since

Feb. 13, 2020

Availability

Academic Institutions and Nonprofits only

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