We narrowed to 21,063 results for: ACE

Showing: 6441 - 6460 of 21063 results

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pBEST-p70a-UTR3-deGFP-6xHis-T500

Plasmid

#92224

Purpose

Expression of deGFP using the p70a promoter and UTR3

Depositor

Lital Alfonta

Article

Schlesinger et al ACS Synth Biol. 2017 Mar 9. doi: 10.1021

Insert

deGFP

Use

Synthetic Biology; Cell free protein synthesis (c…

Tags

6xHis

Expression

Bacterial

Promoter

p70a

Available Since

June 30, 2017

Availability

Academic Institutions and Nonprofits only

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pAS_4xMx PylT C41CA_FLAG_Mx PylRS

Plasmid

#140013

Purpose

Plasmid with 4xMx PylT C41CA cassette and MX1201 PylRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al ACS Chem Biol. 2018 Sep 27. doi: 10.1021

Insert

Mx1201 PylRS (MMALV_RS05520 Methanomethylophilus alvus Mx1201)

Tags

FLAG

Expression

Mammalian

Promoter

EF1

Available Since

May 18, 2020

Availability

Academic Institutions and Nonprofits only

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R0010 (pLacI)_AB

Plasmid

#66004

Purpose

MoClo Basic Part: Controllable promoter - pLacI - lacI regulated (repressed by LacI+CAP. LacI, C0012, inhibited by IPTG) [A:R0010:B]

Depositor

Douglas Densmore

Article

Iverson et al ACS Synth Biol. 2016 Jan 15;5(1):99-103.

Insert

Controllable promoter

Use

Synthetic Biology

Available Since

Jan. 21, 2016

Availability

Academic Institutions and Nonprofits only

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MTK7_013

Plasmid

#123880

Purpose

Encodes the 5' 500 bp hAAVS1 locus homology arm as a type 7 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

RA_hAAVS1

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pEasyG3_hph

Plasmid

#184919

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_hph recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBTK401

Plasmid

#110597

Purpose

BTK Type 8 Bacterial origin (broad-host-range) for golden gate assembly

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

mRFP dropout (RSF1010 origin)

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pCMV DCC mut

Plasmid

#16460

Depositor

Bert Vogelstein

Article

Fearon et al Science. 1990 Jan 5. 247(4938):49-56.

Insert

DCC (DCC Human)

Expression

Mammalian

Mutation

5' end of DCC

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)/HP + AUG-nLuc-3XFLAG

Plasmid

#127322

Purpose

Express 3xFLAG tagged nanoLuciferase (nLuc) from AUG start codon from mRNA with hairpin in 5' UTR

Depositor

Jeremy Wilusz

Article

Kearse et al Genes Dev. 2019 Jun 6. pii: gad.324715.1

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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MTK0_014

Plasmid

#123929

Purpose

Encodes the BxBI attB GFP dropout destination vector as a type 0 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BxBIattB destination vector

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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PAmCherry1-N1-Lyn11

Plasmid

#202683

Purpose

Photoactivatable lipid biosensor

Depositor

Gerry Hammond

Article

Pacheco et al J Cell Biol. 2023 Feb 6;222(2):e20220409

Insert

LYN(1-11):PAmCherry1 (LYN Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pBTK601

Plasmid

#110607

Purpose

BTK assembled plasmid - encodes Cas9 with no targeting sgRNA (used with suicide plasmid for broad-host-range genome alteration)

Depositor

Jeffrey Barrick

Article

Leonard et al ACS Synth Biol. 2018 May 18;7(5):1279-12

Insert

Cas9

Use

Synthetic Biology

Available Since

Feb. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pCRI008-PgpdA-Cas12aScaffold-BsmbI-Cas12aScaffold-TrcpT

Plasmid

#140201

Purpose

Fungal vector for one-step cloning of LbCas12a crRNA arrays and expression. Pgpda and pyroA are BsmbI domesticated.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Type

Empty backbone

Use

CRISPR and Synthetic Biology; Cloning of crrna an…

Tags

dLbCas12a crRNA scaffold

Promoter

PgpdA

Available Since

Sept. 2, 2020

Availability

Academic Institutions and Nonprofits only

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MTK4a_019

Plasmid

#123832

Purpose

Encodes the d2 PEST tag as a Type 4a part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

d2PEST tag

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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pT7-RiboJ-mScarlet-T7

Plasmid

#173227

Purpose

Encodes mScarlet fluorescent protein. To be used in cell-free system

Depositor

Jim Haseloff

Article

Guzman-Chavez et al ACS Synth Biol. 2022 Mar 18;11(3):1114-1

Insert

mScarlet

Use

Cell-free system

Expression

Bacterial

Promoter

T7

Available Since

March 9, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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NET30 pEGFP-N2 (815)

Plasmid

#62011

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

NET30 (MOSPD3 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 23, 2015

Availability

Academic Institutions and Nonprofits only
MTK678_002

Plasmid

#123923

Purpose

Encodes the TU GFP dropout cassette with Ampicillin resistance and bacterial artificial chromosome as a type 678 part to be used in the MTK system

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

BAC-AmpR

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP Rab11a WT pcDNA3.1 Zeo (+)

Plasmid

#206079

Purpose

Rab11a belongs to the Rab family of small GTPases, Rab11 proteins are particularly involved in trafficking through recycling endosomes

Depositor

Annette Dolphin

Article

Tran-Van-Minh et al J Neurosci. 2010 Sep 22;30(38):12856-67.

Insert

RAB11A (RAB11A Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-synPCB2.0

Plasmid

#139477

Purpose

Expression vector for phycocyanobilin (PCB) synthesis in mammalian cells.

Depositor

Kazuhiro Aoki

Article

Uda et al ACS Chem Biol. 2020 Nov 20;15(11):2896-2

Insert

MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Fnr-Fd-T7

Expression

Mammalian

Mutation

V413L

Promoter

CAG promoter

Available Since

April 16, 2020

Availability

Academic Institutions and Nonprofits only

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pPKm-118

Plasmid

#90491

Purpose

Expresses pcDNA3 - CMVmin - 5X UAS - pFR Luciferase

Depositor

Todd Coleman

Article

Kyriakakis et al ACS Synth Biol. 2018 Feb 16;7(2):706-717

Insert

pFR Luciferase Fluc

Expression

Mammalian

Available Since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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JPF0510

Plasmid

#124001

Purpose

Encodes pEF1a driving expression of mAzamiGreen with an mScarlet normalization in a PiggyBac destination vector

Depositor

Hana El-Samad

Article

Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

Insert

PB_pEF1a-NLS-mAzamiGreen-bghpA_pCAG-H2B::mScarlet-rglpA

Expression

Mammalian

Available Since

Dec. 13, 2019

Availability

Academic Institutions and Nonprofits only

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