We narrowed to 10,494 results for: nar

1. pQZ083

   Plasmid

   #106417

   Purpose

   Express Complexin-1 fragment (1-83) in E. coli

   Depositor

   Axel Brunger

   Article

   Zhou et al Nature. 2017 Aug 24;548(7668):420-425. d

   Insert

   complexin-1 fragment 1-83 (Cplx1 Rat, Synthetic)
   

   Use

   Tags

   GST tag

   Expression

   Bacterial

   Mutation

   Fragment 1-83, Codon optimization

   Promoter

   T7

   Available since

   Feb. 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mCer-Vamp2(qv/fw)

   Plasmid

   #53238

   Purpose

   Mutant (Q76V/F77W), tetanus toxin insensitive VAMP2 (synaptobrevin2), N-terminally-tagged with mCerulean, connected by a 4 amino acid linker. For mammalian cells, used with mCit-4aa-SNAP25B for FRET.

   Depositor

   Robert Zucker

   Article

   Degtyar et al J Neurosci. 2013 Mar 27;33(13):5507-23.

   Insert

   VAMP2 (Vamp2 Rat)
   

   Use

   Tags

   4aa-linker and mCerulean

   Expression

   Mammalian

   Mutation

   Mutated Q76 to V; F77 to W.

   Promoter

   Available since

   June 9, 2014

   Availability

   Academic Institutions and Nonprofits only

3. pAcCas12nHS_RV6_PT1_empty

   Plasmid

   #203810

   Purpose

   Plasmid containing a CMV-driven NLS-AcCas12n-PT1-NLS(human-codonoptimized)cassette and a U6-driven sgRNA_V6 cassette(containing BsaI sites for spacer insertion). Carbenicillin resistance.

   Depositor

   Quanjiang Ji

   Article

   Chen et al Mol Cell. 2023 Jun 27:S1097-2765(23)0046

   Insert

   AcCas12nHS_RV6_PT1
   

   Use

   CRISPR

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 22, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET-28a-His6-SUMO-VAMP2

   Plasmid

   #135551

   Purpose

   Express mouse VAMP2 in E. coli BL21, purified via HisPur Ni-NTA Resin

   Depositor

   Jingshi Shen

   Article

   Yu et al Methods Mol Biol. 2019;1860:237-249. doi

   Insert

   VAMP2
   

   Use

   Tags

   6xHis tag, thrombin recognition and cleavage site…

   Expression

   Bacterial

   Mutation

   No ATG start codon

   Promoter

   T7 promoter

   Available since

   Feb. 11, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pGEX 4t1 RARalpha (1-187)

   Plasmid

   #35558

   Depositor

   Jonathan Kurie, Harish Srinivas

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 1-187

   Promoter

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

6. pRRG304-Ndk

   Plasmid

   #99072

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea Ndk (nou darake)

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   Ndk (nou darake) in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 22, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGEX 4T1 RARalpha (186-462)

   Plasmid

   #35559

   Depositor

   Jonathan Kurie, Harish Srinivas

   Article

   Srinivas et al Mol Cell Biol. 2005 Feb;25(3):1054-69.

   Insert

   RARalpha (RARA Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   aa 186-462

   Promoter

   Available since

   April 5, 2012

   Availability

   Academic Institutions and Nonprofits only

8. CAGhCBFA2T2

   Plasmid

   #84363

   Purpose

   FLAG-HA tagged human CBFA2T2

   Depositor

   Danny Reinberg

   Article

   Tu et al Nature. 2016 Jun 8;534(7607):387-90. doi

   Insert

   CBFA2T2 (CBFA2T2 Human)
   

   Use

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRRG227-Wnt1

   Plasmid

   #99073

   Purpose

   dsRNA and riboprobe synthesis for Schmidtea mediterranea Wnt1 (also known as WntP-1)

   Depositor

   Phillip Newmark

   Article

   Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

   Insert

   Wnt1 (also known as WntP-1) in situ probe
   

   Use

   T/a cloning vector for dsrna generation and the g…

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 23, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRV DYRK2

    Plasmid

    #12568

    Depositor

    Anjana Rao

    Article

    Gwack et al Nature. 2006 Jun 1. 441(7093):646-50.

    Insert

    DYRK2 (DYRK2 Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pBEAST-J23101-CocE

    Plasmid

    #114600

    Purpose

    "Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

    Depositor

    Jerome Bonnet

    Article

    Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

    Insert

    CocE
    

    Use

    Synthetic Biology; Cell-free protein synthesis

    Tags

    Expression

    Mutation

    Promoter

    J23101

    Available since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLJM1-Rap2A-3xFLAG

    Plasmid

    #87973

    Purpose

    Expression of Rap2A in mammalian cells

    Depositor

    David Sabatini

    Article

    Cantor et al Cell. 2017 Apr 6;169(2):258-272.e17. doi

    Insert

    RAP2A (RAP2A Human)
    

    Use

    Lentiviral

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. B108-Otx2-IRES-Cre

    Plasmid

    #73993

    Purpose

    B108 enhancer of Blimp1 gene drives Otx2 (isoform b) and Cre expression.

    Depositor

    Connie Cepko

    Article

    Wang et al Dev Cell. 2014 Sep 8;30(5):513-27. doi:

    Insert

    B108-Otx2 (isoform b)-IRES-Cre (Otx2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    July 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMig (Martins Lab)

    Plasmid

    #14125

    Depositor

    L. Miguel Martins

    Article

    Nicke et al Mol Cell. 2005 Dec 9. 20(5):673-85.

    Insert

    IRES-EGFP
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Cla I sites were silenced in the original pRetroS…

    Promoter

    Available since

    March 7, 2007

    Availability

    Academic Institutions and Nonprofits only

15. pRRG199-bmp4

    Plasmid

    #99076

    Purpose

    dsRNA and riboprobe synthesis for Schmidtea mediterranea Bmp4 (also known as bmp)

    Depositor

    Phillip Newmark

    Article

    Roberts-Galbraith et al Proc Natl Acad Sci U S A. 2013 Jan 22;11

    Insert

    Bmp4 (also known as bmp) in situ probe
    

    Use

    T/a cloning vector for dsrna generation and the g…

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Aug. 23, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMK233 (AAVS1 CMV-AtAFB2)

    Plasmid

    #140537

    Purpose

    AAVS1 CMV-AtAFB2

    Depositor

    Masato Kanemaki

    Article

    Yesbolatova et al Nat Commun. 2020 Nov 11;11(1):5701. doi:

    Insert

    CMV-AtAFB2
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. Stx3-mCitrine-mCherry

    Plasmid

    #92426

    Purpose

    Expression of syntaxin-3 C-terminally conjugated to tandem of mCitrine and mCherry.

    Depositor

    Geert van den Bogaart

    Article

    Verboogen et al Elife. 2017 May 19;6. pii: e23525. doi:

    Insert

    Stx3 (Stx3 Rat)
    

    Use

    Tags

    mCherry and mCitrine

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDH-EF1a-tetR-T2A-tSapphire

    Plasmid

    #176312

    Purpose

    construct expresses tet repressor (tetR) to reduce leakiness together with the fluorochrome t-Sapphire

    Depositor

    Irmela Jeremias

    Article

    Liu et al Biomark Res. 2020 Sep 16;8:46. doi: 10.1

    Inserts

    tetR
    T-sapphire
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pC-KIT2

    Plasmid

    #118984

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    mutK2-SP-K1

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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20. pC-KIT4

    Plasmid

    #118986

    Purpose

    Mutational dissection of the G rich region of the c-kit promoter

    Depositor

    Bjorn Hogberg

    Article

    Ducani et al J Am Chem Soc. 2019 Jul 3;141(26):10205-

    Insert

    c-kit proto-oncogene {promoter} (KIT Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    K2-SP-mutK1

    Promoter

    Available since

    June 10, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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