We narrowed to 8,684 results for: App

1. pCEP4-myc-CCR5-VC

   Plasmid

   #98966

   Purpose

   myc-tagged human CCR5 fused to C-terminus of split Venus

   Depositor

   Erik Procko

   Article

   Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

   Insert

   CCR5 (CCR5 Human)
   

   Use

   Tags

   Signal/leader sequence from Influenza A virus HA …

   Expression

   Mammalian

   Mutation

   Full-length, wildtype

   Promoter

   CMV

   Available since

   April 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLCHKO_Ptbp_ex8_3

   Plasmid

   #155083

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of mouse Ptbp exon 8 using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLCHKO_SFRS7_exon_deletion_3

   Plasmid

   #155071

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of SFRS7 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLCHKO_MDM4_exon_deletion_3

   Plasmid

   #155075

   Purpose

   Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases (CHyMErA system)

   Depositor

   Jason Moffat, Benjamin Blencowe

   Article

   Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

   Insert

   (hg)RNA for deletion of MDM4 exon using SpCas9 and LbCas12a nucleases
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Aug. 13, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCR3-Flag-AuroraB KD (K106R)

   Plasmid

   #108488

   Purpose

   expression of Flag-AuroraB kinase death (K106R)

   Depositor

   Susanne Lens

   Article

   Hengeveld et al Mol Cell Proteomics. 2012 May;11(5):47-5

   Insert

   AuroraB (AURKB Human)
   

   Use

   Tags

   Flag

   Expression

   Mammalian

   Mutation

   Kinase death K106A

   Promoter

   CMV

   Available since

   May 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pRK5 GST MetaP2

   Plasmid

   #112750

   Purpose

   express HA-GST-FRB-MetaP2

   Depositor

   Roberto Zoncu

   Article

   Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

   Insert

   HA-GST-FRB-MetaP2 (METAP2 Human)
   

   Use

   Tags

   GST

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Oct. 12, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. Epac-S-H96

   Plasmid

   #170342

   Purpose

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Article

   van der Krogt et al PLoS ONE. 2008 . 3(4):e1916.

   Insert

   CFP(UST)Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLenti PA-mCit-NL

   Plasmid

   #113452

   Purpose

   Lentiviral ProteinA-mCitrine-NanoLuc hybrid-fusion control expression vector (positive control)

   Depositor

   Erich Wanker

   Article

   Trepte et al Mol Syst Biol. 2018 Jul 11;14(7):e8071.

   Insert

   ProteinA-mCitrine-NanoLuc
   

   Use

   Lentiviral and Luciferase

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   hUBbC

   Available since

   Aug. 14, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. sfGFP-Cx43K258stop

   Plasmid

   #69025

   Purpose

   Expresses rat Cx43 (Gja1 CDS) truncated at amino acid 258 and tagged with sfGFP (V206-version) on the N-terminus. CMV promoter.

   Depositor

   David Spray

   Article

   Stout RF et al J Biol Chem. 2015 Aug 11. pii: jbc.M115.

   Insert

   Cx43 (Gja1 Rat)
   

   Use

   Tags

   super folder GFP

   Expression

   Mammalian

   Mutation

   non-monomerized sfGFP fused in frame to N-terminu…

   Promoter

   CMV

   Available since

   Oct. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. DLG3_PDZ_3

    Plasmid

    #103948

    Purpose

    Protein expression and purification of NE-DLG PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    DLG3 (DLG3 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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11. TVBB C-term-Dendra2

    Plasmid

    #169216

    Purpose

    Targeting vector backbone to support a knock-in of Linker-Dendra2 at the C-terminus of a target locus

    Depositor

    Hugo Snippert

    Article

    Bollen et al PLoS Biol. 2022 Jan 28;20(1):e3001527. d

    Insert

    Double SapI flanked Dendra2-P2A-Puro
    

    Use

    Targeting vector backbone

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Jan. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pGEX6P1-HA-MEK1 C121S

    Plasmid

    #53164

    Purpose

    Expresses GST-HA tagged human MEK1 C121S from bacterial expression vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (MAP2K1 Human)
    

    Use

    Tags

    GST and HA

    Expression

    Bacterial

    Mutation

    C121S

    Promoter

    Available since

    June 9, 2014

    Availability

    Academic Institutions and Nonprofits only

13. pSUPER-retro-puro-tetO-Mek1-shRNA

    Plasmid

    #53179

    Purpose

    Expresses shRNA against mouse Mek1 from puromycin resistance retroviral vector

    Depositor

    Christopher Counter

    Article

    Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

    Insert

    MEK1 (Map2k1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    June 19, 2014

    Availability

    Academic Institutions and Nonprofits only

14. EF1a_HOXA1_P2A_Hygro_Barcode

    Plasmid

    #120451

    Purpose

    Barcoded lentiviral vector to express HOXA1 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

    Depositor

    Prashant Mali

    Article

    Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

    Insert

    HOXA1 (HOXA1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    EF1a

    Available since

    Feb. 4, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pADSL-2XStrep

    Plasmid

    #125684

    Purpose

    expresses human ADSL protein with a C-terminal 2XStrep affinity tag

    Depositor

    Stephen Benkovic

    Article

    Liu et al J Proteome Res. 2019 May 3;18(5):2078-20

    Insert

    ADSL (ADSL Human)
    

    Use

    Tags

    2X Strep-Tag II

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 31, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. PTPN13_PDZ_3

    Plasmid

    #103900

    Purpose

    Protein expression and purification of PTPN13 PDZ3 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLJM1 Flag GFP 4E-BP1 Omp25

    Plasmid

    #112760

    Purpose

    express GFP-4EBP1-Omp25

    Depositor

    Roberto Zoncu

    Article

    Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

    Insert

    Flag-GFP-4EBP1-Omp25 (EIF4EBP1 Human)
    

    Use

    Lentiviral

    Tags

    Flag eGFP and Omp25

    Expression

    Mutation

    Promoter

    Available since

    Oct. 16, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. MAGI1_PDZ_2

    Plasmid

    #103899

    Purpose

    Protein expression and purification of MAGI1 PDZ2 domain

    Depositor

    Sachdev Sidhu

    Article

    Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

    Insert

    MAGI1 (MAGI1 Human)
    

    Use

    Tags

    GST

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Aug. 29, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCEP4-CCR5(R5gp120-Hi)

    Plasmid

    #98954

    Purpose

    Mammalian expression plasmid for myc-tagged human CCR5 mutant R5gp120-Hi

    Depositor

    Erik Procko

    Article

    Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

    Insert

    CCR5 (CCR5 Human)
    

    Use

    Tags

    Signal/leader sequence from Influenza A virus HA …

    Expression

    Mammalian

    Mutation

    R5gp120-Hi variant: P8L, I9E, I12M, S17E, P19D, Q…

    Promoter

    CMV

    Available since

    April 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGL3-Basic-Fascin-Promoter (-402-0)

    Plasmid

    #89823

    Purpose

    Promoter luciferase assay

    Depositor

    Shengyu Yang

    Article

    Sun et al J Biol Chem. 2013 Dec 27;288(52):36971-8

    Insert

    Fascin1 (FSCN1 Human)
    

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    SV40

    Available since

    July 5, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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