We narrowed to 7,531 results for: Ski;

1. p21-HA(dPCNA)-NeoR

   Plasmid

   #215108

   Purpose

   Expresses p21-HA(dPCNA) in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CDKN1A (CDKN1A Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   p21(dPCNA) denotes mutations M147A, D149A, F150A.

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGEX-6p-1_GST-DmKHC[1-421]_1xmNeonGreen

   Plasmid

   #196974

   Purpose

   Bacterial expression plasmid for GST-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal mNeonGreen tag and cleavable N-terminal GST tag

   Depositor

   Lukas Kapitein

   Article

   Chen et al Elife. 2022 Jul 5;11:e77892. doi: 10.755

   Insert

   Kinesin Heavy Chain (Khc Fly)
   

   Tags

   GST and mNeonGreen

   Expression

   Bacterial

   Promoter

   tac

   Available Since

   July 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. pDONR223_DOK1_WT

   Plasmid

   #82884

   Purpose

   Gateway Donor vector containing DOK1, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   DOK1 (DOK1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. FFSS-Cyclin D1-NeoR

   Plasmid

   #215087

   Purpose

   Expresses FFSS-Cyclin D1 in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   CCND1 (CCND1 Human)
   

   Tags

   FFSS (FLAG-FLAG-STREP-STREP)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 1, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBABE-NLS-HA-TurboID-PCNA

   Plasmid

   #215074

   Purpose

   Expresses NLS-HA-TurboID-PCNA in mammalian cells from a retroviral vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   PCNA (PCNA Human)
   

   Use

   Retroviral

   Tags

   NLS-HA-TurboID

   Expression

   Mammalian

   Promoter

   LTR

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL3-sgRANGAP.C1-CAG-Cas9-T2A-mCherry-P2A-Puro

   Plasmid

   #216249

   Purpose

   Express Cas9 with CAG promoter to improve the expression in hES cells and sgRANGAP.C1 to tag endogenous RANGAP1 C-terminus

   Depositor

   Elina Ikonen

   Article

   Li et al Genome Biol. 2024 Feb 26;25(1):58. doi:

   Insert

   Cas9 and sgRANGAP.C1 (RANGAP1 Human)
   

   Use

   CRISPR; Endogenous tagging

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   March 27, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pDONR223_CCND1_WT

   Plasmid

   #82891

   Purpose

   Gateway Donor vector containing CCND1, part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   CCND1 (CCND1 Human)
   

   Use

   Gateway entry vector

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. pDONR223_RBM15B_WT_V5

   Plasmid

   #83010

   Purpose

   Gateway Donor vector containing RBM15B, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

   Depositor

   David Root, Matthew Meyerson, Jesse Boehm

   Article

   Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

   Insert

   RBM15B (RBM15B Human)
   

   Use

   Gateway entry vector

   Mutation

   R593K; p.1_327deleted

   Promoter

   None

   Available Since

   April 24, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCIBN-hTRF1-tagRFP-T

   Plasmid

   #103811

   Purpose

   BLInCR 'Localizer' construct that marks telomeres and is targeted by a PHR-tagged effector upon illumination with blue light; can be visualized without triggering PHR recruitment

   Depositor

   Karsten Rippe

   Article

   Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

   Insert

   CIBN-hTRF1-tagRFP-T (CIB1, TERF1 Human, Mustard Weed)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 9, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAG-2dV-Camui (T286A)

    Plasmid

    #220367

    Purpose

    T286A phosphodead mutant of pCAG-2dV-Camui

    Depositor

    Ryohei Yasuda

    Article

    Jain et al Nature. 2024 Oct 9. doi: 10.1038/s41586-

    Insert

    mVenus(Y145W)-mVenus(Y145W)-rat CaMK2 alpha(T286A)-mEGFP(A206K) (Camk2a Synthetic, Rat)
    

    Tags

    mEGFP(A206K) and mVenus(Y145W)-mVenus(Y145W)

    Expression

    Mammalian

    Mutation

    changed Threonine 286 to Alanine

    Promoter

    CAG

    Available Since

    Oct. 9, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pDONR223_CGREF1_WT

    Plasmid

    #82895

    Purpose

    Gateway Donor vector containing CGREF1, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    CGREF1 (CGREF1 Human)
    

    Use

    Gateway entry vector

    Mutation

    deletion of AA 233-249

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. pDONR223_FCGR3B_WT

    Plasmid

    #82896

    Purpose

    Gateway Donor vector containing FCGR3B, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    FCGR3B (FCGR3B Human)
    

    Use

    Gateway entry vector

    Mutation

    N65S

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. pDONR223_PIH1D1_WT_V5

    Plasmid

    #82943

    Purpose

    Gateway Donor vector containing PIH1D1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    PIH1D1 (PIH1D1 Human)
    

    Use

    Gateway entry vector

    Mutation

    M9L, G10E, V224I, P287L

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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14. TRCN0000338399

    Plasmid

    #78156

    Purpose

    shXPO1 targeting CDS

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    XPO1 (XPO1 Human)
    

    Use

    Lentiviral and RNAi

    Promoter

    hU6 and hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

15. pDONR223_KEAP1_p.G524C

    Plasmid

    #82779

    Purpose

    Gateway Donor vector containing KEAP1, part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    KEAP1 (KEAP1 Human)
    

    Use

    Gateway entry vector

    Mutation

    G524C; C624S

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. pET28a_DmKHC[1-421]-SNAP-6xHis

    Plasmid

    #196975

    Purpose

    Bacterial expression plasmid for His tag-based purification of Drosophila melanogaster kinesin heavy chain [1-421] with C-terminal SNAP tag and C-terminal 6xHis tag

    Depositor

    Lukas Kapitein

    Article

    Deguchi et al Science. 2023 Mar 10;379(6636):1010-1015

    Insert

    Kinesin Heavy Chain (Khc Fly)
    

    Tags

    6xHis and SNAP

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDONR223_RNH1_WT_V5

    Plasmid

    #82995

    Purpose

    Gateway Donor vector containing RNH1, used as an over expression control for gene expression analysis. Part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Matthew Meyerson, Jesse Boehm

    Article

    Berger et al Cancer Cell. 2016 Aug 8;30(2):214-28. do

    Insert

    RNH1 (RNH1 Human)
    

    Use

    Gateway entry vector

    Mutation

    R188H

    Promoter

    None

    Available Since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. t1-405

    Plasmid

    #166127

    Purpose

    For expression of human talin-1 head (residues 1-405) in E. coli. N-terminal His6-tag, Xpress-epitope (DLYDDDDK) and enterokinase cleavage site for tag removal.

    Depositor

    Vesa Hytönen

    Article

    Kukkurainen et al J Cell Sci. 2020 Oct 12;133(19). pii: 13

    Insert

    Tln1Head (1-405) (TLN1 Human)
    

    Tags

    His6-tag, Xpress-epitope (DLYDDDDK) and enterokin…

    Expression

    Bacterial

    Mutation

    residues 1-405 only

    Available Since

    April 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pU6-pegRNA-HEK3-CTTins-px330-scaffold

    Plasmid

    #180017

    Purpose

    Transiently expressing a pegRNA to introduce HEK3 CTT insertion in human cells. It has a sgRNA scaffold from px330.

    Depositor

    Dirk Hockemeyer

    Article

    Li et al Elife. 2022 Sep 7;11:e79208. doi: 10.755

    Insert

    Prime editing pegRNA for HEK3-CTTins, with px330 scaffold (EPHA8 Synthetic)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Human U6

    Available Since

    Sept. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pLVX-TetOne-FFSS-Cyclin D1 (T286A)

    Plasmid

    #215147

    Purpose

    Expresses FFSS-Cyclin D1 (T286A) in mammalian cells from an inducible lentiviral vector.

    Depositor

    Michele Pagano

    Article

    Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

    Insert

    CCND1 (CCND1 Human)
    

    Use

    Lentiviral

    Tags

    FFSS (FLAG-FLAG-STREP-STREP)

    Expression

    Mammalian

    Mutation

    With T286A mutation.

    Promoter

    TRE3GS

    Available Since

    April 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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