We narrowed to 17,256 results for: tes

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CAG-Cremyc-2A-GFP

Plasmid

#116879

Purpose

Express Cre enzyme in mammalian cells with floxed genes to achieve somatic knockout. GFP is simultaneously expressed for convenient tracking the transfected cells.

Depositor

Qiang Lu

Publication

Geng et al Nat Commun. 2018 Jul 13;9(1):2707. doi:

Insert

Cremyc-2A-GFP

Expression

Mammalian

Available since

May 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pTNS2

Plasmid

#87802

Purpose

For constructing NusA-SUMO-fusion proteins with N-terminal StrepII tag and C-terminal His tag using "blunt-end" cloning.

Depositor

Yu-Zhu Zhang

Publication

Zhang et al Mol Biotechnol. 2017 May;59(4-5):159-167

Type

Empty backbone

Tags

6xHis and Strep II

Expression

Bacterial

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

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neurod1:cre; cmlc2:EGFP

Plasmid

#173480

Purpose

cre is driven by -5kb neurod1 promoter. The vector contain a cmlc2:EGFP maker

Depositor

John Rawls

Publication

Ye et al Cell Host Microbe. 2021 Feb 10;29(2):179

Insert

Cre

Use

Zebrafish expression

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pGAN213

Plasmid

#129270

Purpose

Control for measuring background levels of yTRAP reporter protein

Depositor

Ahmad Khalil

Publication

Newby et al Cell. 2017 Nov 2;171(4):966-979.e18. doi

Insert

mNeonGreen

Expression

Yeast

Promoter

min pCYC1

Available since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pNL4-3 envFS eGFP gag2xFS

Plasmid

#101341

Purpose

1st frameshift is CG nucleotide insertion in MA at nt 832. 2nd is a CTAG addition in CA at nt 1508.

Depositor

Jeremy Luban

Publication

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pRL-TK let7 D

Plasmid

#11327

Depositor

Phil Sharp

Publication

Doench et al Genes Dev. 2004 Mar 1. 18(5):504-11.

Insert

2 binding sites for let-7a miRNA

Use

Luciferase

Tags

Rr-luc

Mutation

Mutation D from figure 4A of paper.

Available since

Feb. 15, 2006

Availability

Academic Institutions and Nonprofits only
pLentiCRISPRv2_cGAS_gRNA_3

Plasmid

#235531

Purpose

gRNA against human cGAS

Depositor

Nicolas Manel

Publication

Lahaye et al Cell. 2025 May 29:S0092-8674(25)00556-2.

Insert

cGAS (CGAS Human)

Use

Lentiviral

Available since

July 17, 2025

Availability

Academic Institutions and Nonprofits only

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lentiCRISPRv2_sgCtrl#2

Plasmid

#174149

Purpose

Lentiviral vector expressing Cas9 and a control sgRNA that targets a safe harbor site

Depositor

Julien Sage

Publication

Chaikovsky et al Nature. 2021 Apr;592(7856):794-798. doi:

Insert

Control sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available since

Sept. 10, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pRF-HlyIIR

Plasmid

#49361

Depositor

Christopher Voigt

Publication

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

HlyIIR repressor

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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pLX-EF1a_GFP

Plasmid

#221481

Purpose

GFP lentiviral overexpression

Depositor

Matthew Meyerson

Publication

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

EGFP

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pIRESpuroN3xFLAG-His-CDK5RAP3

Plasmid

#204555

Purpose

Expresses 3xFLAG-His-tagged CDK5RAP3 in mammalian cells

Depositor

Masaaki Komatsu

Publication

Ishimura et al Nat Commun. 2022 Dec 21;13(1):7857. doi:

Insert

CDK5RAP3 (AMY2A Human)

Expression

Mammalian

Mutation

none

Available since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pCAGGS-FLAG-CaMKIIB

Plasmid

#186826

Purpose

Expresses FLAG-CaMKIIB in mammalian cells

Depositor

Hung-Chun Chang

Publication

Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

Insert

Camk2b (Camk2b Mouse)

Tags

FLAG

Expression

Mammalian

Available since

April 3, 2023

Availability

Academic Institutions and Nonprofits only

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pSEVA221T7RNAP

Plasmid

#167974

Purpose

Expresses T7RNAP in E. coli

Depositor

Luis Ángel Fernández

Publication

Alvarez et al Nat Commun. 2020 Dec 22;11(1):6436. doi:

Insert

T7RNAP (T7p07 Synthetic)

Promoter

TetR-Ptet

Available since

June 17, 2021

Availability

Academic Institutions and Nonprofits only

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pLux-LmrA

Plasmid

#49375

Depositor

Christopher Voigt

Publication

Stanton et al Nat Chem Biol. 2013 Dec 8. doi: 10.1038/

Insert

LmrA repressor

Use

Synthetic Biology

Expression

Bacterial

Available since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pMCRi

Plasmid

#207524

Purpose

Plasmid for CRISPRi; oriV(pRO1600/ColE1), xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Depositor

Pablo Ivan Nikel

Publication

Kozaeva et al Nucleic Acids Res. 2024 Jan 5:gkad1236.

Insert

xylS (cured of BsaI-sites), Pm→ Sp dCas9, PEM7 →sgRNA; SmR/SpR

Use

Pseudomonas vector

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltalinker

Plasmid

#157870

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the linker

Depositor

Nicolas Fawzi

Publication

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaliner

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2_N_deltaCTD

Plasmid

#157871

Purpose

express MBP tagged SARS-CoV-2 Nucleocapsid protein without the C-terminal domain

Depositor

Nicolas Fawzi

Publication

Perdikari et al EMBO J. 2020 Nov 17:e106478. doi: 10.152

Insert

N (N SARS-CoV-2)

Tags

MBP

Expression

Bacterial

Mutation

deltaCTD

Available since

Nov. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pSTAR-mOrange2-sfGFP

Plasmid

#115693

Purpose

Generation of CD-tagged libraries with an mOrange2-sfGFP Mammalian Cell-Optimized Fluorescent Timer

Depositor

David Suter

Publication

Alber et al Mol Cell. 2018 Aug 17. pii: S1097-2765(1

Insert

SA-mOrange2-sfGFP-SD

Use

Lentiviral

Available since

Sept. 21, 2018

Availability

Academic Institutions and Nonprofits only

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LentiGuidPuro-hTP63_sgRNA-1

Plasmid

#88854

Purpose

CRISPR KO of Trp63

Depositor

Joan Massague

Publication

Wang et al Cell Stem Cell. 2017 Jan 5;20(1):70-86.

Insert

Trp63

Use

CRISPR and Lentiviral

Expression

Mammalian

Available since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pUC18-mini-Tn7T-Gm-LacZ20

Plasmid

#65027

Purpose

GmR mini-Tn7 vector, for construction of B-galactosidase protein fusions, frame 2. See Depositor Comments below for information regarding the lacZ sequence.

Depositor

Herbert Schweizer

Publication

Choi et al Nat Protoc. 2006;1(1):153-61.

Type

Empty backbone

Tags

lacZ

Expression

Bacterial

Available since

Nov. 2, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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