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pNRCreCNAT

Plasmid

#234430

Purpose

Picochlorum celeri CloNat insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal

Depositor

Matthew Posewitz

Article

Burch et al Front Microbiol. 2025 Jul 14;16:1588625.

Insert

Cre recombinase

Use

CRISPR, Cre/Lox, and Synthetic Biology ; Green al…

Mutation

Picochlorum celeri endoglucanase intron insertion

Promoter

P celeri nitrate reductase

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pNRCreBle

Plasmid

#234431

Purpose

Picochlorum celeri ShBle insert with nitrate-inducible Cre recombinase and loxP sites for marker and Cre removal

Depositor

Matthew Posewitz

Article

Burch et al Front Microbiol. 2025 Jul 14;16:1588625.

Insert

Cre recombinase

Use

CRISPR, Cre/Lox, and Synthetic Biology ; Green al…

Mutation

Picochlorum celeri endoglucanase intron insertion

Promoter

P celeri nitrate reductase

Available Since

Aug. 22, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV-Tnnt2-HA-Luciferase

Plasmid

#227803

Purpose

cardiomyocytes-specific plasmid with Tnnt2 promotor, expressing the coding sequence of luciferase and a HA tag

Depositor

Yuxuan Guo

Article

Chen et al Fundam Res. 2025 Jun 30;5(6):2993-3000.

Insert

HA-Luciferase

Use

AAV

Expression

Mammalian

Promoter

Tnnt2

Available Since

Aug. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pMT-EGFP-V5

Plasmid

#240240

Purpose

Expression of EGFP-V5 under control of copper-inducible promoter.

Depositor

Justin Bosch, Norbert Perrimon

Article

Bosch et al bioRxiv [Preprint]. 2025 Jul 11:2025.07.

Insert

EGFP-V5

Expression

Insect

Promoter

MT

Available Since

July 11, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-3xFlag-hGpr75

Plasmid

#236746

Purpose

Express 3xFlag N-terminal tagged wild-type human GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

Insert

GPR75 (GPR75 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

July 8, 2025

Availability

Academic Institutions and Nonprofits only

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T7pro-tRNA-ITSIle intron-T7tVar1-pRS426

Plasmid

#239294

Purpose

Expresses the tRNAIle intron bearing the 5' GGGAGA initially transcribed sequence (ITS); Plasmid #2 of the tet-on overexpression system

Depositor

Anita Hopper

Article

Nostramo et al Mol Cell. 2025 Feb 20;85(4):726-741.e6.

Inserts

T7 promoter
ITS-tRNAIle intron
T7 terminator - Var1

Expression

Yeast

Available Since

June 23, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA595 - pBA904 Puro-T2A-mCherry

Plasmid

#238171

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mCherry

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pRCA594 - pBA904 Puro-T2A-BFP

Plasmid

#238170

Purpose

Lentiviral CRISPR guide vector expressing a non-targeting sgRNA with cs1 incorporated in the loop of the sgRNA constant region for 10X Direct Capture

Depositor

Thomas Norman

Article

Southard et al Nat Genet. 2025 Aug 6. doi: 10.1038/s415

Insert

Non-targeting sgRNA

Use

CRISPR and Lentiviral

Tags

mTagBFP2

Available Since

June 3, 2025

Availability

Academic Institutions and Nonprofits only

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pBOB-3xFlag-mGpr75

Plasmid

#236744

Purpose

Express 3xFlag N-terminal tagged wild-type mouse GPR75 transiently via transfection or stably via lentivirus-mediated infection in mammalian cells.

Depositor

Zhao Zhang

Article

Jiang et al J Clin Invest. 2024 Aug 13;134(19):e1821

Insert

Gpr75 (Gpr75 Mouse)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

May 27, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT221: pR6K-Donor(ZooTsy)_H1:12_E1:135_E2:20_H2:12

Plasmid

#200874

Purpose

ZooTsy donor harboring KanR cargo.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

ZooTsy

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT401: pHelper(AjTn6022)_QF-separated_TniQ-K361D

Plasmid

#200861

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Mutation

TniQ-K361D

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pSCT400: pHelper(AjTn6022)_QF-separated_TniQ-K348D

Plasmid

#200860

Purpose

Inducible expression of AjTn6022 proteins.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

AjTn6022

Expression

Bacterial

Mutation

TniQ-K348D

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP270: pHelper(CyCAST)_Shuffled-spacer_ΔTnsD

Plasmid

#200845

Purpose

Inducible expression of CyCAST proteins (except TnsD) with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP267: pHelper(CyCAST)_PSP1_ΔTniQ

Plasmid

#200842

Purpose

Inducible expression of CyCAST proteins (except TniQ) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP269: pHelper(CyCAST)_PSP1_ΔTnsD

Plasmid

#200844

Purpose

Inducible expression of CyCAST proteins (except TnsD) with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP116: pHelper(CyCAST)_PSP1

Plasmid

#200840

Purpose

Inducible expression of CyCAST proteins with crRNA targeting CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pMSIP122: pHelper(CyCAST)_Shuffled-spacer

Plasmid

#200841

Purpose

Inducible expression of CyCAST proteins with control crRNA for CyPSP1.

Depositor

Feng Zhang

Article

Faure et al Mol Cell. 2023 Jun 15;83(12):2122-2136.e

Insert

CyCAST

Expression

Bacterial

Available Since

March 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-AncHolozoan Sox

Plasmid

#231528

Purpose

Expresses reconstructed Ancestral Holozoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Holozoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-AncMetazoan Sox

Plasmid

#231527

Purpose

Expresses reconstructed Ancestral Metazoan Sox in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral Metazoan Sox HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE2-TetO-AncSoxCEF

Plasmid

#231526

Purpose

Expresses reconstructed Ancestral SoxCEF in mammalian cells, for lentivirus generation

Depositor

Ralf Jauch

Article

Gao et al Nat Commun. 2024 Nov 14;15(1):9868. doi:

Insert

Ancestral SoxCEF HMG flanked with N- and C-termini of mSox2

Use

Lentiviral

Available Since

March 19, 2025

Availability

Academic Institutions and Nonprofits only

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