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Flag-HA-USP1

Plasmid

#22596

Depositor

Wade Harper

Article

Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

Insert

USP1 (USP1 Human)

Use

Retroviral

Tags

FLAG and HA

Expression

Mammalian

Mutation

None

Available Since

Nov. 13, 2009

Availability

Academic Institutions and Nonprofits only

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Myc-E-Syt1

Plasmid

#66833

Purpose

Mammalian Expression, Bacterial Expression

Depositor

Pietro De Camilli

Article

Giordano et al Cell. 2013 Jun 20;153(7):1494-509. doi:

Insert

E-Syt1 (ESYT1 Human)

Tags

Myc

Expression

Mammalian

Available Since

July 21, 2015

Availability

Academic Institutions and Nonprofits only

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pAG414GPD-dCas9-VPR

Plasmid

#63801

Purpose

pAG414 series plasmid with GPD promoter driving expression of dCas9-VPR; cerevisiae vector

Depositor

George Church

Article

Chavez et al Nat Methods. 2015 Mar 2. doi: 10.1038/nm

Insert

dCas9-VPR

Use

CRISPR and Synthetic Biology

Tags

VPR (VP64-p65-Rta)

Expression

Yeast

Promoter

GPD

Available Since

April 14, 2015

Availability

Academic Institutions and Nonprofits only

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pTECH-chPylRS(IPYE)

Plasmid

#99222

Purpose

Expression plasmid encoding chPylRS(IPYE) and pylT

Depositor

Dieter Söll

Article

Bryson et al Nat Chem Biol. 2017 Oct 16. doi: 10.1038

Insert

Plpp chPyl(IPYE)RS; PproK pylT

Expression

Bacterial

Mutation

Contains activating mutations V31I, T56P, H62Y, A…

Promoter

Plpp and PproK

Available Since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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3xHA-TurboID-NLS_pCDNA3

Plasmid

#107171

Purpose

expresses 3xHA-tagged TurboID in the mammalian nucleus

Depositor

Alice Ting

Article

Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

Insert

TurboID (BirA mutant)

Expression

Mammalian

Mutation

Q65P, I87V, R118S, E140K, Q141R, S150G, L151P, V1…

Available Since

May 4, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3-N-Flag-Caspase-1

Plasmid

#75128

Purpose

Expresses N-terminal Flag-tagged Caspase-1 in mammalian cells

Depositor

Bruce Beutler

Article

Shi et al Nat Immunol. 2016 Mar;17(3):250-8. doi:

Insert

caspase 1 (Casp1 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

P341S in Caspase-1 (please see depositor comments…

Promoter

CMV

Available Since

May 19, 2017

Availability

Academic Institutions and Nonprofits only

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AAVS1_gRNA

Plasmid

#196139

Purpose

gRNA targeting the AAVS1 locus in a third generation Cas9 backbone with GFP

Depositor

Jason Sheltzer

Article

Girish et al Science. 2023 Jul 6:eadg4521. doi: 10.11

Insert

AAVS1 gRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 10, 2023

Availability

Academic Institutions and Nonprofits only

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pBR322-sgRNA-pvc18-20-Pdp1_NTD-Cre

Plasmid

#243748

Purpose

PVC cargo/regulatory region - deleted toxin cargos (Pdp1/Pnf) - vegfa sgRNA - Cre loaded into the PVC tube via Pdp1 (NTD)

Depositor

Feng Zhang

Article

Kreitz et al Nat Biotechnol. 2025 Aug 12. doi: 10.103

Insert

vegfa sgRNA-pvc18-20-Pdp1_NTD-Cre

Expression

Bacterial

Available Since

Sept. 24, 2025

Availability

Academic Institutions and Nonprofits only

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pHAGE-3FLAG-EEA1

Plasmid

#176491

Purpose

For lentiviral transduction of 3FLAG-EEA1

Depositor

Wade Harper

Article

Park et al Nat Commun. 2022 Oct 16;13(1):6112. doi:

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFLAG

Available Since

May 31, 2022

Availability

Academic Institutions and Nonprofits only

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Stx5S-GFP

Plasmid

#209847

Purpose

Expresses Stx5S-GFP in mammalian cells

Depositor

Geert van den Bogaart

Article

Linders et al Nat Commun. 2021 Oct 28;12(1):6227. doi:

Insert

STX5 (STX5 Human)

Tags

eGFP

Expression

Mammalian

Mutation

Lacks amino acids 1-54, encodes short isoform of …

Promoter

CMV

Available Since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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