165,528 results

1. pGTM_COV2_NSP5_004_SUMO

   Plasmid

   #190062

   Purpose

   Expresses SARS-CoV-2 3CLpro for the expression of the active mature protease

   Depositor

   Gaetano Montelione

   Article

   Mazzei et al STAR Protoc. 2023 May 5;4(2):102326. doi

   Insert

   3CLpro (ORF1ab SARS-CoV-2)
   

   Tags

   6X-His-Sumo

   Expression

   Bacterial

   Promoter

   T7 lac

   Available Since

   June 3, 2024

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pCMV-T7-ABEmax(7.10)-SpG-P2A-EGFP (RTW4562)

   Plasmid

   #140002

   Purpose

   CMV and T7 promoter expression plasmid for human codon optimized ABEmax(7.10) A-to-G base editor with SpG(D10A/D1135L/S1136W/G1218K/E1219Q/R1335Q/T1337R) and P2A-EGFP

   Depositor

   Benjamin Kleinstiver

   Article

   Walton et al Science. 2020 Apr 17;368(6488):290-296.

   Insert

   human codon optimized ABEmax(7.10) SpCas9 variant named SpG with P2A-EGFP
   

   Use

   CRISPR; In vitro transcription; t7 promoter

   Tags

   P2A-EGFP

   Expression

   Mammalian

   Mutation

   nSpCas9=D10A; SpG=D1135L/S1136W/G1218K/E1219Q/R13…

   Promoter

   CMV and T7

   Available Since

   March 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. GST-AR-624-919

   Plasmid

   #104200

   Purpose

   Expresses human AR-624-929 ligand binding domain in pGEX-5X-1 as GST fusion protein

   Depositor

   Elizabeth Wilson

   Article

   He et al Mol Cell Biol. 2003 Mar;23(6):2135-50.

   Insert

   human androgen receptor aa 624-919 (AR Human)
   

   Tags

   GST

   Expression

   Bacterial

   Available Since

   Dec. 6, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pJMP1356

   Plasmid

   #119276

   Purpose

   for cloning new sgRNAs

   Depositor

   Jason Peters, Oren Rosenberg, Carol Gross

   Article

   Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

   Insert

   dcas9
   

   Expression

   Bacterial

   Mutation

   D10A and H840A

   Available Since

   March 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Plasmid

   #175777

   Purpose

   pSMART Tmem192-3X HA (targeting vector for genomic tagging)

   Depositor

   Wade Harper

   Article

   Eapen et al eLife. 2021 Sep 29;10. pii: 72328. doi:

   Insert

   TMEM192 (TMEM192 Human)
   

   Use

   CRISPR

   Available Since

   Feb. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pcDNA-RLuc8

   Plasmid

   #87121

   Purpose

   Mammalian expression vector expressing mutant version of the reporter gene-Renilla Luciferase (Rluc8), which is more stable in mouse serum and has more light output.

   Depositor

   Sanjiv Sam Gambhir

   Article

   Loening et al Protein Eng Des Sel. 2006 Sep;19(9):391-

   Insert

   Renilla Luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Mutation

   A55T, C124A, S130A, K136R, A143M, M185V, M253L, a…

   Promoter

   CMV

   Available Since

   April 5, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pBK2044-AAV-2xSp1-2xNFkB-EFSNC-dSaCas9-KRAB-MECP2

   Plasmid

   #223164

   Purpose

   2nd gen. vector for expression of KRAB and truncated MECP2 with dSaCas9 and empty gRNA scaffold

   Depositor

   Boris Kantor

   Article

   Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

   Insert

   KRAB-MECP2 (MECP2 Human)
   

   Use

   AAV and CRISPR

   Tags

   3xFLAG

   Expression

   Mammalian

   Mutation

   Encodes aa 204-310 (MECP2)

   Promoter

   EF1a

   Available Since

   Aug. 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p2T-CMV-ABE8e_dNRCH-BlastR

   Plasmid

   #232720

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   ABE8e-dCas9(NRCH)
   

   Expression

   Mammalian

   Mutation

   within Cas9 D10A H840A

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSNAPf-N1

   Plasmid

   #58187

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Tags

   SNAP tag

   Expression

   Mammalian

   Available Since

   Jan. 13, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

10. AP1

    Plasmid

    #179678

    Purpose

    Accessory plasmids

    Depositor

    David Liu

    Article

    Mok et al Nat Biotechnol. 2022 Sep;40(9):1378-1387

    Insert

    [SD8] gIII
    

    Expression

    Bacterial

    Available Since

    April 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. GFP-polyQ74-NLS

    Plasmid

    #231892

    Purpose

    Forms nuclear HTT partial exon 1 Q74-NLS aggregates

    Depositor

    Lukas Kapitein

    Article

    Korsten et al J Cell Biol. 2024 Nov 4;223(11):e2023071

    Insert

    HTT partial exon 1 Q74-NLS (HTT Human)
    

    Tags

    EGFP and SV40 NLS

    Expression

    Mammalian

    Mutation

    CAG repeat expansion

    Available Since

    Feb. 13, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pCINeoFlag ALIX

    Plasmid

    #89859

    Purpose

    Expresses full length Flag-tagged ALIX in mammalian cells; internal ID WISP03-308

    Depositor

    Wesley Sundquist

    Article

    Zhai et al PLoS One. 2011;6(12):e27466. doi: 10.137

    Insert

    ALIX (PDCD6IP Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-HA-mSTING

    Plasmid

    #214157

    Purpose

    Transient expression of STING

    Depositor

    Nan Yan

    Article

    Chu et al Nature. 2021 Aug;596(7873):570-575. doi:

    Insert

    STING (Sting1 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 5, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX307-APN-blast

    Plasmid

    #158455

    Purpose

    Constitutive lentiviral expression of human APN.

    Depositor

    Alejandro Chavez, Sho Iketani

    Insert

    APN (ANPEP Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    Aug. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pX330A-FBL/PITCh

    Plasmid

    #63671

    Purpose

    Expresses Cas9 nuclease, FBL-specific gRNA, and the PITCh-gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Nat Protoc. 2016 Jan;11(1):118-33. doi:

    Insert

    humanized S. pyogenes Cas9
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Promoter

    CBh

    Available Since

    Dec. 18, 2015

    Availability

    Academic Institutions and Nonprofits only

16. pMVV099

    Plasmid

    #215305

    Purpose

    Expresses sfGFP under induction with the Cuminic Acid Marionette Cassette (CymRAM + PCymRC-sfGFP) with a BBR1 backbone.

    Depositor

    Brian Pfleger

    Article

    Venkataraman et al ACS Synth Biol. 2023 Dec 15;12(12):3623-

    Insert

    sfGFP
    

    Use

    Synthetic Biology

    Tags

    None

    Expression

    Bacterial

    Promoter

    PCymRC

    Available Since

    May 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. MSCV-pU6-(BbsI)-CcdB-(BbsI)-Pgk-Puro-T2A-BFP

    Plasmid

    #86457

    Purpose

    Mouse stem cell retroviral vector including a puromycin resistance and BFP gene. sgRNA targets can be cloned in between the BbsI sites

    Depositor

    Ralf Kuehn

    Article

    Chu et al Proc Natl Acad Sci U S A. 2016 Nov 1;113

    Insert

    BFP
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    Pgk

    Available Since

    Feb. 10, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3 Flag beta-1-adrenergic-receptor

    Plasmid

    #14698

    Depositor

    Robert Lefkowitz

    Article

    Tang et al Proc Natl Acad Sci U S A. 1999 Oct 26. 9

    Insert

    B1AR (ADRB1 Human)
    

    Tags

    Flag

    Expression

    Mammalian

    Available Since

    June 19, 2007

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pRS314-URA3

    Plasmid

    #11004

    Depositor

    Francisco Malagon

    Insert

    URA3 (URA3 Budding Yeast)
    

    Expression

    Bacterial and Yeast

    Mutation

    Wild type

    Available Since

    April 28, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. LentiGuide Puro-P2A-EGFP

    Plasmid

    #137729

    Purpose

    For simple determination of the multiplicity of infection (MOI) of a lentiviral CRISPR library by checking EGFP expression (still allowing for puro selection).

    Depositor

    Fredrik Wermeling

    Article

    Panda et al Proc Natl Acad Sci U S A. 2020 Jan 24. p

    Type

    Empty backbone

    Use

    CRISPR, Lentiviral, and Synthetic Biology

    Tags

    EGFP

    Expression

    Bacterial and Mammalian

    Available Since

    Feb. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge