We narrowed to 11,589 results for: EMB

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pcDNA-mGFP-Eps15DN

Plasmid

#223621

Purpose

Transient expression of human Eps15 with mGFP tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

Eps15 (EPS15 Human)

Tags

mGFP

Expression

Mammalian

Mutation

Eps15 _ 95-295aa

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pHR-CD86-mTFP1

Plasmid

#223606

Purpose

Lentiviral expression of human CD86 with mTFP1 tag in mammalian cells

Depositor

Enfu Hui

Article

Zhao et al Immunity. 2023 Jun 13;56(6):1187-1203.e1

Insert

CD86 (CD86 Human)

Use

Lentiviral; mammalian expression

Tags

mTFP1

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

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pMRS-dCas9

Plasmid

#220193

Purpose

mini-Tn7 dCas9 expression vector

Depositor

Larry Halverson

Article

Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

Insert

Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9

Use

CRISPR

Expression

Bacterial

Mutation

Pseudomonas aeruginosa codon optimized

Promoter

promoterless

Available Since

Aug. 26, 2024

Availability

Academic Institutions and Nonprofits only

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MSCV-mNINJ1-D53A-GFP

Plasmid

#208778

Purpose

MSCV vector expressing D53A mouse NINJ1

Depositor

Petr Broz

Article

Ramos et al EMBO J. 2024 Apr;43(7):1164-1186. doi: 1

Insert

NINJ1 (Ninj1 Mouse)

Use

Retroviral

Tags

GFP

Mutation

Changed aspartic acid 53 to alanine (D53A)

Available Since

Aug. 2, 2024

Availability

Academic Institutions and Nonprofits only

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pET28a-SnoopTag-SpyTag-(AffiHER2)x3

Plasmid

#216281

Purpose

Three affibodies to HER2 linked in a chain for bacterial expression. SnoopTag and SpyTag allow isopeptide bond formation to their cognate Catcher

Depositor

Mark Howarth

Article

Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

Insert

SnoopTag-SpyTag-(AffiHER2)x3

Use

Affinity Reagent/ Antibody

Tags

His6, SnoopTag, and SpyTag

Expression

Bacterial

Promoter

T7 promoter

Available Since

June 26, 2024

Availability

Academic Institutions and Nonprofits only

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pKH162_FLAG-APEX2-YIPF3 LIR mut

Plasmid

#214981

Purpose

Flag-APEX2 tagged LIR mutant YIPF3

Depositor

Wade Harper

Article

Hickey et al Nature. 2023 Nov;623(7985):167-174. doi:

Insert

FLAG-APEX2-YIPF3_mut (YIPF3 Human)

Use

Lentiviral

Mutation

LIR mutant

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pCDH-V2-CD20-Puro

Plasmid

#209756

Purpose

Lentiviral transfer plasmid to express the 5'-UTR and CDS of the human CD20 gene, MS4A1. Specifically, the variant 2 mRNA isoform.

Depositor

Andrei Thomas-Tikhonenko

Article

Ang et al Blood. 2023 Sep 8:blood.2023020400. doi:

Insert

MS4A1 (MS4A1 Human)

Use

Lentiviral

Available Since

Nov. 30, 2023

Availability

Academic Institutions and Nonprofits only

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ku70 insUP+pPGI+NAT+tNOS+ku70 insD (SBE217)

Plasmid

#195049

Purpose

integration cassette for ku70 deletion and expression of NAT gene under pPGI for characterization

Depositor

Petri-Jaan Lahtvee

Article

Bonturi et al Metab Eng Commun. 2022 May 23;15:e00200.

Insert

NAT

Expression

Bacterial

Promoter

pPGI

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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pCAG eGFP-Oct6

Plasmid

#203790

Purpose

Competitive assay of Oct4 and Oct6 binding to DNA motifs.

Depositor

Thorsten Wohland

Article

Mistri et al EMBO Rep. 2015 Sep;16(9):1177-91. doi: 1

Insert

Pou3f1 (Pou3f1 Mouse)

Tags

mEGFP

Expression

Mammalian

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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PH-miRFP-FRB

Plasmid

#193568

Purpose

Expresses PH domain from PLC delta1 tagged with miRFP703 and FRB mutant (T2098L) in mammalian cells.

Depositor

Justin Taraska

Article

Obashi et al Nat Commun. 2023 Feb 9;14(1):732. doi: 1

Insert

PH domain from PLC delta1 (Plcd1 Rat)

Tags

FRB (T2098L) and miRFP703

Expression

Mammalian

Mutation

PH domain

Promoter

CMV

Available Since

Feb. 10, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-DrCav1

Plasmid

#194290

Purpose

Multisite gateway entry clone for expression of codon optimised zebrafish caveolin1 with fusion tag at the N-terminus. Parton lab clone KRT

Depositor

Rob Parton

Article

Wu et al Dev Cell. 2023 Mar 13;58(5):376-397.e4.

Insert

caveolin1 (cav1.S Frog)

Use

Multisite gateway entry vector

Available Since

Dec. 16, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG2_zeo

Plasmid

#184913

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_zeo recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Dec. 13, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_msfGFP-SEPT7 Gmut2

Plasmid

#180425

Purpose

mammalian expression of human SEPT7 Gmut2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN7 (SEPTIN7 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT7 H279D

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1-14-GFP10

Plasmid

#180343

Purpose

mammalian expression of human SEPT9_i1 fused to GFP10

Depositor

Manos Mavrakis

Article

Martins et al J Cell Biol. 2023 Mar 6;222(3):e20220301

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

GFP10

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 1, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG2_hph

Plasmid

#184915

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG2_hph recombines in vivo with a PCR product from pEasyG2_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pEasyG3_zeo

Plasmid

#184917

Purpose

Template to generate via PCR two gRNAs for expression in S. cerevisiae. The PCR product from pEasyG3_zeo recombines in vivo with a PCR product from pEasyG3_mic.

Depositor

Jeferson Gross

Article

Jacobus et al ACS Synth Biol. 2022 Nov 18;11(11):3886-

Insert

gRNA scaffold

Use

CRISPR

Expression

Bacterial

Available Since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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His-GST-Cys-M87 Spastin

Plasmid

#184804

Purpose

Bacterial expression of M87 Spastin (A2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

M87 Spastin (SPAST Human)

Tags

His6-GST

Expression

Bacterial

Mutation

A2C

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pET15b MOSPD2 [282-490]

Plasmid

#186469

Purpose

Expression of human MOSPD2 (MSP domain from residue 282 to 490), fused to a 6HIS tag, in bacteria

Depositor

Catherine Tomasetto

Article

Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

Insert

MOSPD2 motile sperm domain containing 2 (MOSPD2 Human)

Tags

6HIS

Expression

Bacterial

Mutation

MSP domain [282-490]

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pGEMHE-TNKS-2

Plasmid

#154149

Purpose

For making TNKS-2 RNA

Depositor

Carmen Williams

Article

Gambini et al Dev Cell. 2020 Jun 8;53(5):545-560.e7. d

Insert

Tankyrase 2 (TNKS2 Human)

Use

In vitro transcription

Tags

FLAG

Promoter

T7

Available Since

June 22, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO - RBM28 E5 WT minigene

Plasmid

#169269

Purpose

Mammalian vector for constitutive expression of RBM28 Exon 5 WT minigene

Depositor

Susan Baserga

Article

Bryant et al Proc Natl Acad Sci U S A. 2021 May 11;11

Insert

RBM28 E5 WT minigene (RBM28 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 8, 2022

Availability

Academic Institutions and Nonprofits only

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