We narrowed to 15,882 results for: LEA

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PD-L1-miSFIT-Ce67

Plasmid

#124686

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-L1 (CD274 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 6, 2019

Availability

Academic Institutions and Nonprofits only

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PET-eSpCas9-plus-NLS-6xHis

Plasmid

#207380

Purpose

Expression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cells

Depositor

Ervin Welker

Article

Kulcsar et al Nat Commun. 2023 Sep 16;14(1):5746. doi:

Insert

eSpCas9-plus

Use

CRISPR

Tags

6xHis

Expression

Bacterial

Mutation

K848A, R1060A, amino acids 1005-1013 replaced wit…

Promoter

T7

Available Since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-CAG-FLEX-ArgiNLS-AausFP1

Plasmid

#220602

Purpose

Cre-dependent expression of a single-cell discriminating version of AausFP1 fluorescent protein.

Depositor

Sam Golden

Article

Szelenyi et al Proc Natl Acad Sci U S A. 2024 Aug 6;121

Insert

AausFP1

Use

AAV and Cre/Lox

Tags

ArgiNLS

Expression

Mammalian

Promoter

CAG

Available Since

July 31, 2024

Availability

Academic Institutions and Nonprofits only

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pCEP4-BG505.SOSIP-TM

Plasmid

#111844

Purpose

Mammalian expression plasmid for BG505 SOSIP.664 fused to a transmembrane tether

Depositor

Erik Procko

Article

Heredia et al J Virol. 2019 Mar 20. pii: JVI.00219-19.

Insert

HIV-1 Env (BG505 SOSIP.664)

Tags

CD5 leader sequence and TM helix from MHC class I

Expression

Mammalian

Mutation

Codon-optimized synthetic gene; has SOSIP mutatio…

Promoter

CMV

Available Since

March 25, 2019

Availability

Academic Institutions and Nonprofits only

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PD-1-miSFIT-1x

Plasmid

#124679

Purpose

Tuning expression of PD-1

Depositor

Tudor Fulga

Article

Michaels et al Nat Commun. 2019 Feb 18;10(1):818. doi:

Insert

PD-1 (PDCD1 Human)

Expression

Mammalian

Promoter

minimal CMV

Available Since

May 7, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-nCas9

Plasmid

#163555

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)

Available Since

Jan. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST-DHFR F[3]-N (LEU2)

Plasmid

#177798

Purpose

Gateway destination vector to insert genes of interest having a N-terminal DHFR F[3] fusion for DHFR-PCA/BFG-PCA assays.

Depositor

Christian Landry

Article

Evans-Yamamoto et al Nucleic Acids Res. 2022 Feb 7. pii: 6523

Type

Empty backbone

Expression

Yeast

Promoter

ADH1

Available Since

Jan. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_UdgX-APOBEC1-UdgX-HF-nCas9

Plasmid

#163559

Purpose

Mammalian CG-to-GC base editing

Depositor

David Liu

Article

Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

Insert

UdgX-APOBEC1-UdgX-HF-nCas9

Use

CRISPR

Expression

Mammalian

Mutation

nCas9 (D10A)HF (N497A, R661A, Q695A, Q926A)

Available Since

Jan. 4, 2021

Availability

Academic Institutions and Nonprofits only

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PEA1

Plasmid

#176527

Purpose

Delivers all prime editing nickase components in a single plasmid

Depositor

Paul Thomas

Article

Adikusuma et al Nucleic Acids Res. 2021 Sep 17. pii: 637

Insert

CbH-Cas9(H840A)-RT, hU6-pegRNA (Bbs1 gate), hU6-sgRNA (Bbs1 gate)

Use

CRISPR

Expression

Mammalian

Promoter

CbH for Cas9, hU6 for gRNAs

Available Since

Dec. 16, 2021

Availability

Academic Institutions and Nonprofits only

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2_T7-mScarlet3-KRas

Plasmid

#225929

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the K-Ras membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al bioRxiv 2024.11.12.623055

Insert

mScarlet3-KRas

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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pOTTC414 - pAAV EF1a hPREP

Plasmid

#59967

Purpose

An AAV packaging vector that expresses hPREP under control of the EF1a promoter.

Depositor

Brandon Harvey

Article

Savolainen et al Neurobiol Dis. 2014 Aug;68:1-15. doi: 10

Insert

Prolyl oligopeptidase (PREP Human)

Use

AAV

Expression

Mammalian

Promoter

EF1a

Available Since

Oct. 8, 2014

Availability

Academic Institutions and Nonprofits only

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pEMS2155

Plasmid

#141065

Purpose

AAV plasmid with SYN1 promoter driving expression of EmGFP. Contains WPRE.

Depositor

Elizabeth Simpson

Article

Galvan et al Hum Gene Ther. 2021 Apr 16. doi: 10.1089

Insert

SYN1-EmGFP-WPRE

Use

AAV

Promoter

human Synapsin

Available Since

April 28, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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px459 sgAtg5

Plasmid

#175023

Purpose

CRISPR-Cas9 plasmid targeting exon 2 of human Atg5.

Depositor

David Tumbarello

Article

Assar et al Front Cell Dev Biol. 2020 Aug 4;8:733. d

Insert

ATG5 (ATG5 Human)

Expression

Mammalian

Available Since

Oct. 6, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP/golgin-160/3DE

Plasmid

#180631

Purpose

Expression of caspase-resistant golgin-160

Depositor

Carolyn Machamer

Article

Mancini et al J Cell Biol. 2000 May 1;149(3):603-12. d

Insert

golgin-160/3DE (GOLGA3 Human)

Tags

EGFP

Expression

Mammalian

Mutation

D59E, D139E, D311E

Available Since

March 24, 2022

Availability

Academic Institutions and Nonprofits only

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TV-hPITX3-forward

Plasmid

#22073

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2009 Sep;27(9):851-7. do

Insert

PITX3 (ASMD Human)

Use

Cre/Lox; Zfn donor

Tags

eGFP

Available Since

Oct. 5, 2009

Availability

Academic Institutions and Nonprofits only

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ROSA26(MultiFPsΔPuro)

Plasmid

#140759

Purpose

Targeting vector to Gt(ROSA)26 locus for conditional expression of distinct FPs (Venus, mCherry, and mCerulean) responding to each site-specific-recombinase activity (Cre, Dre, and phiC31o).

Depositor

Hideyuki Okano

Article

Serizawa et al Development. 2019 Nov 4;146(21). pii: de

Inserts

puromycin-N-acetyltransferase
Venus
mCherry
mCerulean

Use

Mouse Targeting

Promoter

CAGGS

Available Since

June 16, 2020

Availability

Academic Institutions and Nonprofits only

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pHS0766 pET45b(+) His14-MBP-TEV-AloTnpB-2 3' extension

Plasmid

#176542

Purpose

Bacterial expression of His14-MBP-TEV-AloTnpB-2 with 3' extension derived from locus

Depositor

Feng Zhang

Article

Altae-Tran et al Science. 2021 Oct;374(6563):57-65. doi:

Inserts

MBP tag
TnpB

Tags

3' extension derived from endogenous locus a…

Expression

Bacterial

Available Since

Oct. 18, 2021

Availability

Academic Institutions and Nonprofits only

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pSynapsin_NLS_GFP_P2A_SNAP_NR1

Plasmid

#170458

Purpose

Expresses SNAP tagged NR1 in neuronal cells

Depositor

Shigeki Watanabe

Article

Li et al Nat Commun. 2021 Jan 29;12(1):677. doi:

Insert

NR1 (Grin1 Rat)

Use

Lentiviral

Tags

SNAP

Expression

Mammalian

Promoter

Synapsin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX-UAP56

Plasmid

#157661

Purpose

Expresses UAP56 in E.coli cell

Depositor

Byung Il Lee

Article

Bong et al Nucleic Acids Res. 2020 Jun 19;48(11):63

Insert

Spliceosome RNA helicase DDX39B (DDX39B Human)

Tags

GST tag

Expression

Bacterial

Mutation

deleted amino acids 1-43

Available Since

Aug. 20, 2020

Availability

Academic Institutions and Nonprofits only

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PITX3-eGFP-PGK-Puro

Plasmid

#31942

Depositor

Rudolf Jaenisch

Article

Hockemeyer et al Nat Biotechnol. 2011 Jul 7. doi: 10.1038

Insert

PITX3 (PITX3 Human)

Use

Targeting donor

Tags

eGFP

Available Since

Sept. 13, 2011

Availability

Academic Institutions and Nonprofits only

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