We narrowed to 15,599 results for: LEA

1. pLV-EF1a-V5-Emerin

   Plasmid

   #120246

   Purpose

   Expresses V5-tagged Emerin in lentiviral vector

   Depositor

   Larry Gerace

   Article

   Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

   Insert

   Emerin (Emd Mouse)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 9, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. lenti-sgRNA(MS2)_zeo_hPDX1_promoter_guide1

   Plasmid

   #176838

   Purpose

   To induce human PDX1 expression by recruiting SAM complex to PDX1 promoter

   Depositor

   Rudolf Jaenisch

   Article

   Ma et al Cell Stem Cell. 2022 Apr 13. pii: S1934-

   Insert

   sgPDX1
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   Nov. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSN69

   Plasmid

   #100125

   Purpose

   expresses putative cMYC DBD with a HA tag

   Depositor

   Mark Ptashne

   Article

   Narayan et al Cell Rep. 2017 Aug 15;20(7):1585-1596. d

   Insert

   mCerulean-P2A-putative cMYC DBD with a HA tag (MYC Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Insert corresponds to the DBD of cMYC

   Promoter

   Available since

   Sept. 19, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-HA-DNM2-W525L

   Plasmid

   #155263

   Purpose

   Mammalian expression of rat DNM2 fused to HA

   Depositor

   David Rubinsztein

   Article

   Puri et al Dev Cell. 2020 Apr 20;53(2):154-168.e6.

   Insert

   dynamin 2 (Dnm2 Rat)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Tryptophan 525 to Leucine

   Promoter

   Available since

   June 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBabe-ER-E2F2-dRb

   Plasmid

   #62805

   Purpose

   expresses an inducible E2F2 mutant that lacks the Rb binding domain

   Depositor

   Gerardo Ferbeyre

   Article

   Vernier et al Genes Dev. 2011 Jan 1;25(1):41-50. doi:

   Insert

   E2F2 (E2F2 Human)
   

   Use

   Retroviral

   Tags

   HA-Er

   Expression

   Mammalian

   Mutation

   deletion of amino acids 302-437, including the Rb…

   Promoter

   Available since

   April 2, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. HOXB1 (human) HIS-tag pET

   Plasmid

   #8520

   Depositor

   Corey Largman

   Article

   Shen et al Nucleic Acids Res 1996 Mar 1;24(5):898-9

   Insert

   HOXB1 (HOXB1 Human)
   

   Use

   Tags

   His and T7

   Expression

   Bacterial

   Mutation

   PCR from cDNA clone

   Promoter

   Available since

   July 6, 2006

   Availability

   Academic Institutions and Nonprofits only

7. pClneoMyc MOB1 T12A

   Plasmid

   #47936

   Purpose

   expresses Myc tagged human MOB1 containing T12A mutation

   Depositor

   Yutaka Hata

   Article

   Bao et al Genes Cells. 2009 Dec;14(12):1369-81. do

   Insert

   MOB1A (MOB1A Human)
   

   Use

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   T12A

   Promoter

   CMV

   Available since

   Sept. 27, 2013

   Availability

   Academic Institutions and Nonprofits only

8. pcDNA4-hB90

   Plasmid

   #18968

   Depositor

   Mariano Viapiano

   Article

   Hu et al J Biol Chem. 2008 Sep 5;283(36):24848-59

   Insert

   Brevican C-terminus (BCAN Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Human brevican signal peptide aa 1-23 followed by…

   Promoter

   Available since

   Dec. 12, 2008

   Availability

   Academic Institutions and Nonprofits only

9. pX330-NQL007-NANOG-sgRNA

   Plasmid

   #175555

   Purpose

   For transient expression of spCas9-nuclease and a sgRNA targeting the mouse NANOG locus.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nuclease and sgRNA against mouse NANOG STOP Codon
   

   Use

   Mouse Targeting

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pFLAG-4S Tetherin

    Plasmid

    #41073

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S74, S84, 137S, and 144S

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

11. p2Flag CMV2-ZO-2-N

    Plasmid

    #27418

    Depositor

    Marius Sudol

    Article

    Oka et al Biochem J. 2010 Nov 25. 432(3):461-72.

    Insert

    Zonula Occludens 2 (TJP2 Human)
    

    Use

    Tags

    2xFlag

    Expression

    Mammalian

    Mutation

    Deletion of the carboxy-terminus of ZO-2, so only…

    Promoter

    Available since

    April 12, 2011

    Availability

    Academic Institutions and Nonprofits only

12. pMKO.1-shEsco2

    Plasmid

    #160957

    Purpose

    Esco2 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Esco2 shRNA (Esco2 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pLVX-EF1a-EGFP-TA Bik-IRES-Puromycin

    Plasmid

    #133026

    Purpose

    Expresses EGFP-tagged tail anchor sequence of Bik

    Depositor

    David Andrews

    Article

    Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

    Insert

    Bcl-2-interacting killer (BIK Human)
    

    Use

    Lentiviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    deleted amino acids 1-110

    Promoter

    Human elongation factor 1 alpha

    Available since

    Feb. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFLAG-137.144S Tetherin

    Plasmid

    #41072

    Depositor

    Paul Spearman

    Article

    Hammonds et al J Virol. 2012 Feb;86(4):2259-72. Epub 20

    Insert

    Tetherin (BST2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    137S, 144S

    Promoter

    CMV

    Available since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

15. HS-DN-Cep152-mKate2

    Plasmid

    #105943

    Purpose

    heat shock inducible transgenesis

    Depositor

    Caren Norden

    Article

    Strzyz et al Dev Cell. 2015 Jan 26;32(2):203-19. doi:

    Insert

    Cep152 (CEP152 Human)
    

    Use

    Tags

    mKate2

    Expression

    Bacterial

    Mutation

    dominant negative (AA 1-512)

    Promoter

    Available since

    Feb. 6, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pMKO.1-shBub1

    Plasmid

    #160948

    Purpose

    Bub1 shRNA in pMKO.1 retroviral vector

    Depositor

    Matthew Steinhauser

    Article

    Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

    Insert

    Bub1 shRNA (Bub1 Mouse)
    

    Use

    RNAi and Retroviral

    Tags

    Expression

    Mutation

    Promoter

    U6

    Available since

    Dec. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. MAP1LC3A-V1

    Plasmid

    #98569

    Purpose

    Expresses MAP1LC3A-V1

    Depositor

    Shahenda El-Naggar

    Article

    Nassar et al Sci Rep. 2017 Aug 14;7(1):8022. doi: 10.

    Insert

    MAP1LC3A (MAP1LC3A Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 26, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCin4-3xFLAG-MKL1 D444-500

    Plasmid

    #19839

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 D444-500 (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    deleted amino acids 444-500

    Promoter

    Available since

    March 16, 2009

    Availability

    Academic Institutions and Nonprofits only

19. pBB0323-TEV-His12

    Plasmid

    #137039

    Purpose

    expression clone from T7 promoter for full-length (signal peptide containing) B. burgdorferi BB0323 with C-terminal TEV-protease-cleavable His12

    Depositor

    Debra Hansen

    Article

    Robertson et al Sci Rep. 2019 Nov 26;9(1):17606. doi: 10

    Insert

    AAC66700.1
    

    Use

    Tags

    His12 (TEV protease cleavable)

    Expression

    Bacterial

    Mutation

    wild type, full-length protein, contains signal p…

    Promoter

    T7

    Available since

    April 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCin4-3xFLAG-MKL1 T450A

    Plasmid

    #19843

    Depositor

    Ron Prywes

    Article

    Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

    Insert

    MKL1 T450A (MRTFA Human)
    

    Use

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    changed Threonine 450 to Alanine

    Promoter

    Available since

    Dec. 15, 2008

    Availability

    Academic Institutions and Nonprofits only