We narrowed to 26,576 results for: RON

1. FokI(inactive)-mCherry-LacI-NLS

   Plasmid

   #215100

   Purpose

   Expresses FokI(inactive)-mCherry-LacI-NLS in mammalian cells.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   FokI(inactive)-mCherry-LacI-NLS
   

   Expression

   Mammalian

   Mutation

   FokI(inactive) mutant D69A.

   Promoter

   CMV

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pVD1 M1_1-pTRNA-scf 2.1 (GB2603)

   Plasmid

   #160568

   Purpose

   tRNA and scaffold 2.1 scRNA aptamer Ms2 for the assembly of GBoligomers for a single position of a polycistronic tRNA-gRNA regulated by the U6-26 or U6-1 promoter.

   Depositor

   Diego Orzaez

   Article

   Selma et al Plant Biotechnol J. 2019 Sep;17(9):1703-

   Insert

   M1_1-pTRNA-scf 2.1
   

   Use

   CRISPR

   Mutation

   BsaI and BsmBI sites removed

   Available Since

   March 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV hSyn mCh-IRES-BoNT/B(1-146)-iLID

   Plasmid

   #122982

   Purpose

   AAV plasmid with human synapsin promoter driving mCh and BoNT/B amino acids 1-146 fused to iLID(V416I), separated with an IRES element. Co-express with SSPB-BoNT(147-441, Y365A) for sPA-BoNT

   Depositor

   Matthew Kennedy

   Article

   Liu et al Neuron. 2019 Jan 15. pii: S0896-6273(19)

   Insert

   mCh-IRES-BoNT/B(1-146)-iLID
   

   Use

   AAV

   Tags

   mCh

   Expression

   Mammalian

   Mutation

   iLID contains long lived V416I mutation

   Promoter

   human synapsin

   Available Since

   March 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. CDKN1A (p21) targeting gRNA

   Plasmid

   #215319

   Purpose

   Expresses gRNA targeting CDKN1A (p21) and pSpCas9(BB)-2A-GFP in mammalian cells from the px458 vector.

   Depositor

   Michele Pagano

   Article

   Rona et al Mol Cell. 2024 Feb 29:S1097-2765(24)0012

   Insert

   hSpCas9
   

   Use

   CRISPR

   Tags

   3XFLAG and GFP

   Expression

   Mammalian

   Promoter

   Cbh

   Available Since

   April 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-Ef1a-Coff/Fon-sRGECO

   Plasmid

   #137128

   Purpose

   Intersectional viral expression of sRGECO in cells expressing Flp AND NOT Cre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Coff/Fon-sRGECO
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   E217D

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-Ef1a-Con/Fon/Von GCaMP 6m

   Plasmid

   #137165

   Purpose

   Intersectional viral expression of GCaMP6M in cells expressing Cre AND Flp AND Vcre

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   3x-GCaMP6M
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   Removed RSET tag

   Promoter

   Ef1a

   Available Since

   Nov. 4, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-Ef1a-Con/Foff 2.0-BFP

   Plasmid

   #137130

   Purpose

   Intersectional viral expression of BFP in cells expressing Cre AND NOT Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Has Service

   AAV8

   Insert

   Con/Foff 2.0-BFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   EF1a

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pAAV-nEF-Con/Fon-bREACHes-EYFP

   Plasmid

   #137145

   Purpose

   Intersectional viral expression of bREACHes-EYFP in cells expressing both Cre AND Flp

   Depositor

   Karl Deisseroth, INTRSECT 2.0 Project

   Article

   Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

   Insert

   Con/Fon-bREACHes-EYFP
   

   Use

   AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

   Mutation

   n/a

   Promoter

   nEF

   Available Since

   June 25, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. Flrt3-AP-His

   Plasmid

   #71949

   Purpose

   Expresses the extracellular region of the FLRT3 protein, C-terminally fused to alkaline phosphatase + 6X histidine tag.

   Depositor

   Woj Wojtowicz

   Article

   Visser et al Elife. 2015 Dec 3;4. pii: e08149. doi: 1

   Insert

   Flrt3 (Flrt3 Mouse)
   

   Tags

   AP-His

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pRGEN-CMV-VPR-WT CjCas9 V-WT

    Plasmid

    #169914

    Purpose

    Expression of VPR-WT CjCas9 fusion protein to induce orthogonal genes knock out and activation.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    CjCas9
    

    Use

    CRISPR

    Tags

    2xSV40NLS, SV40NLS-HA, and VPR (VP64-p65-Rta)

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pRepair-SYFP2-CTNNB1

    Plasmid

    #153432

    Purpose

    Homology Directed Repair construct for N-terminal tagging of hsCTNNB1 with SYFP2

    Depositor

    Renee van Amerongen

    Article

    de Man et al Elife. 2021 Jun 30;10. pii: 66440. doi:

    Insert

    CTNNB1 homology arms and SYFP2 coding sequence (CTNNB1 Human)
    

    Use

    CRISPR; Hdr donor template

    Tags

    SYFP2

    Available Since

    Sept. 1, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. pAAV.CMV.CD86.C-HA

    Plasmid

    #154848

    Purpose

    Expresses murine CD86 protein (T-lymphocyte activation antigen) with HA-tag at C-terminus

    Depositor

    Kevin Park

    Article

    Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

    Insert

    Cluster of Differentiation 86 (Cd86 Mouse)
    

    Use

    AAV

    Tags

    HA-tag

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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13. pZE21/UBP1/ClpS

    Plasmid

    #98566

    Purpose

    Expresses truncated codon-opt S. cerevisiae UBP1 and E. coli ClpS in an artificial operon

    Depositor

    George Church

    Article

    Kunjapur et al Proc Natl Acad Sci U S A. 2018 Jan 16;11

    Inserts

    Truncated ubiquitin cleavase, codon optimized
    ATP-dependent Clp protease adapter protein
    

    Expression

    Bacterial

    Mutation

    In a synthetic operon downstream of UBP1 and Remo…

    Promoter

    Same as UBP1 (operon) and Tet promoter (aTc induc…

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLEX307-Bat-CoV-HKU9-3CL-C144A

    Plasmid

    #160285

    Purpose

    Allows for constituitive mammalian expression of the Bat-CoV-HKU9 3CL protease with C145A mutation via transfection or lentivirus

    Depositor

    Alejandro Chavez

    Article

    Resnick et al J Virol. 2021 Jun 24;95(14):e0237420. do

    Insert

    3CL Protease
    

    Use

    Lentiviral

    Expression

    Mammalian

    Mutation

    C144A

    Promoter

    EF1a

    Available Since

    Oct. 6, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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15. MSCV-dGFP-JARID1C A388P

    Plasmid

    #74778

    Purpose

    retroviral expression of JARID1C A388P

    Depositor

    Giovanni Tonon

    Article

    Rondinelli et al J Clin Invest. 2015 Dec;125(12):4625-37.

    Insert

    JARID1C (KDM5C Human)
    

    Use

    Retroviral

    Expression

    Mammalian

    Mutation

    A388P

    Available Since

    May 3, 2016

    Availability

    Academic Institutions and Nonprofits only

16. human SLC12A5-V5-HA-2A-Luciferase repair template

    Plasmid

    #158576

    Purpose

    For targeted insertion of V5-HA-2A-Luciferase reporter before the stop codon of the human SLC12A5 gene that encodes neuronal chloride transporter KCC2 protein.

    Depositor

    Rudolf Jaenisch

    Article

    Tang et al Sci Transl Med. 2019 Jul 31;11(503). pii

    Insert

    human SLC12A5 5' homology arm-V5-HA-2A-Luciferase-3' homology arm (SLC12A5 Human)
    

    Tags

    V5 and HA on the endogenous KCC2 protein product

    Expression

    Mammalian

    Available Since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-nEF-Coff/Fon-bREACHes-EYFP

    Plasmid

    #137147

    Purpose

    Intersectional viral expression of bREACHes-EYFP in cells expressing Flp AND NOT Cre

    Depositor

    Karl Deisseroth, INTRSECT 2.0 Project

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Insert

    Coff/Fon-bREACHes-EYFP
    

    Use

    AAV, Cre/Lox, and Synthetic Biology ; Flp/frt

    Mutation

    n/a

    Promoter

    nEF

    Available Since

    June 25, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pOPINF Ta-sro1 WWE-PARP

    Plasmid

    #192550

    Purpose

    E. coli expression vector for Triticum aestivum sro1 WWE-PARP domains (amino acids 2-434)

    Depositor

    Lennart Wirthmueller

    Article

    Vogt et al Plant Cell. 2022 Aug 18. pii: 6671231. d

    Insert

    SIMILAR TO RCD ONE 1 (SRO1) WWE-PARP domains, cultivar Shanrong No. 3 (SR3)
    

    Tags

    His6, 3C protease cleavage site

    Expression

    Bacterial, Insect, and Mamm…

    Promoter

    T7 promoter

    Available Since

    Dec. 2, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pVD1 Multiplexing Edit E4 (GB2241)

    Plasmid

    #160563

    Purpose

    tRNA and scaffold for the assembly of GBoligomers for position [4-5] of a polycistronic tRNA-gRNA.

    Depositor

    Diego Orzaez

    Article

    Vazquez-Vilar et al Front Plant Sci. 2021 Jul 1;12:689937. d

    Insert

    Multiplexing Edit (E4)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    BsaI and BsmBI sites removed

    Available Since

    March 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pMCh2008_pLenti_Syn_mChe-cdc42E7-E63'UTR

    Plasmid

    #118622

    Purpose

    expression of mChe-tagged cdc42E7-E6-3'UTR under pSyn promoter for primary neurons

    Depositor

    Marina Chekulaeva

    Article

    Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

    Insert

    cdc42E7-E6 3'UTR (Cdc42 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    changed ttgcttt to cggtaag (496 - 502 nt in 3…

    Promoter

    synapsin I (rat)

    Available Since

    March 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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