We narrowed to 11,379 results for: ENA

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pRDB_156

Plasmid

#216090

Purpose

Cas9 [Sp] CRISPRa targeting CD274, positive control

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

CD274 guide

Use

CRISPR and Lentiviral; Positive controls

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pHLP712

Plasmid

#203741

Purpose

Integration of OsTIR1(F74A)-3xMyc at NEUT5L locus

Depositor

Mark Hall

Article

Milholland et al mSphere. 2023 Aug 18:e0028323. doi: 10.1

Insert

OsTIR1

Use

Candida albicans integrating vector

Tags

3xMyc

Mutation

Codon 74 changed from Phe to Ala (F74A)

Promoter

ACT1 (C. albicans)

Available Since

Aug. 16, 2023

Availability

Academic Institutions and Nonprofits only

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pGCP123-GFP_g1

Plasmid

#153518

Purpose

sgRNA to GFP gene (NT) under nisin-inducible nisA promoter, barcoded

Depositor

Kimberly Kline

Article

Afonina et al mBio. 2020 Oct 20;11(5):e01101-20. doi:

Insert

PnisA-sgRNA(GFP_g1)_dCas9 scaffold (barcoded)

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

July 28, 2020

Availability

Academic Institutions and Nonprofits only

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pRedCm

Plasmid

#176225

Purpose

LambdaRed-expressing plasmid carrying the chloramphenicol acetyltransferase gene repressible by the CI repressor of lambda phage

Depositor

Sergey Sineoky

Article

Bubnov et al Nucleic Acids Res. 2022 Aug 26;50(15):89

Insert

Lambda Red operon under control of PrhaB promoter and cat under control of PL promoter of lambda phage

Use

Synthetic Biology

Expression

Bacterial

Promoter

PrhaB and PL

Available Since

Nov. 23, 2021

Availability

Academic Institutions and Nonprofits only

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Cas12j-8-puro

Plasmid

#194966

Purpose

The plasmid expresses human codon-optimized Cas12j-8 and puromycin resistence gene.

Depositor

Yongming Wang

Article

Wang et al Sci Adv. 2023 Feb 10;9(6):eabo6405. doi:

Type

Empty backbone

Use

AAV

Expression

Mammalian

Available Since

Feb. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1185

Plasmid

#119255

Purpose

rfp "test" strain

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

dcas9

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Jan. 7, 2019

Availability

Academic Institutions and Nonprofits only

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Sec61b-HA-mNG21-10

Plasmid

#141213

Purpose

Membrane Tethered Split Fluorescent Reporter for ER Retragrade

Depositor

Wayne Lencer

Article

Luong et al Mol Biol Cell. 2019 Nov 27:mbcE19070375.

Insert

Sec61b-HA-mNG21-10

Use

Lentiviral

Tags

HA

Expression

Mammalian

Available Since

July 31, 2020

Availability

Academic Institutions and Nonprofits only

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pMV_AA089

Plasmid

#216105

Purpose

gRNA expression for Cas12a (guide only)

Depositor

John Doench

Article

McGee et al Cell Genom. 2024 Mar 13;4(3):100519. doi

Insert

gRNA expression

Use

CRISPR and Lentiviral; Assembled vector

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pET28-T7-NlaCas3-NLS-6xHis

Plasmid

#180214

Purpose

Expressing Neisseria lactamica Type I-C Cas3 with NLS and His tag on the C terminus for protein purification in E.Coli

Depositor

Yan Zhang

Article

Tan et al Mol Cell. 2022 Feb 17;82(4):852-867.e5.

Insert

NlaCas3c

Tags

6xHis and NLS

Expression

Bacterial

Promoter

T7

Available Since

March 15, 2022

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-ChRger3-TS-EYFP

Plasmid

#127240

Purpose

High photocurrent, low-light sensitive channelrhodopsin (ChRger3) for optogenetic activation with systemic delivery or for low-light activation. Driven by human Synapsin I promoter.

Depositor

Viviana Gradinaru

Article

Bedbrook et al Nat Methods. 2019 Oct 14. pii: 10.1038/s

Insert

ChRger3-TS-EYFP

Use

AAV

Tags

EYFP and SpyTag

Expression

Mammalian

Promoter

hSyn1

Available Since

Sept. 27, 2019

Availability

Academic Institutions and Nonprofits only

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