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p2T-CMV-HNHx-ABEmax7.10HD-BlastR

Plasmid

#176477

Purpose

A-to-G base editor with alternative targeting scope

Depositor

Gerald Schwank

Publication

Villiger et al Mol Ther Nucleic Acids. 2021 Aug 26;26:5

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

HNHx-ABEmax7.10HD

Use

CRISPR

Expression

Mammalian

Promoter

CMV

Available since

Nov. 17, 2021

Availability

Academic Institutions and Nonprofits only

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pCS2plus-mVenus1-155-GGGS-TDP-43

Plasmid

#162611

Purpose

Allows for transcription of mVenus fluorophore half fused to TDP-43 for injection into zebrafish embryos for BiFC assays

Depositor

Marco Morsch

Publication

Don et al Mol Neurobiol. 2021 May;58(5):2061-2074.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

TARDBP (TARDBP Human)

Expression

Mammalian

Available since

June 10, 2021

Availability

Academic Institutions and Nonprofits only

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pFlox_N-Mau2_BSD_FKBPF36V_EYFP

Plasmid

#163880

Purpose

Acute Depletion of Mau2-EYFP

Depositor

Cornelis Murre

Publication

Zhu et al Genes Dev. 2021 Jan 1;35(1-2):65-81. doi

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Mau2 (Mau2 Mouse)

Expression

Mammalian

Available since

Feb. 19, 2021

Availability

Academic Institutions and Nonprofits only

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INCTbiosyn-p35S(rc)2_4_5-GFP

Plasmid

#127520

Purpose

Plasmid has an inverted CaMV 35S promoter sequence (reverse complement) flanked by attB and attP attachment sites of integrases 2, 4, and 5. EGFP coding sequence is in the forward orientation.

Depositor

Martin Bonamino

Publication

Gomide et al Commun Biol. 2020 May 22;3(1):255. doi:

Insert

CaMV 35S reverse complement promoter sequence flanked by attB/attP Integrase 2, 4 and 5 attachment sites.

Expression

Plant

Available since

May 6, 2020

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Scarlet I-FRET 10-Venus

Plasmid

#139366

Purpose

FRET Standard

Depositor

Richard Day

Publication

Day et al PLoS One. 2021 Dec 23;16(12):e0261660. d

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

Scarlet 10AA Venus

Expression

Mammalian

Available since

April 28, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-Centrin3

Plasmid

#136807

Purpose

Mammalian expression of the centrosomal protein Centrin3 N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP-Centrin3

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

April 6, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTetD-SNAP-FOP

Plasmid

#136797

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

March 27, 2020

Availability

Academic Institutions and Nonprofits only

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pEBTet-SNAP-FOP

Plasmid

#136796

Purpose

Mammalian expression of the centrosomal protein FGFR N-terminally fused to SNAP-tag

Depositor

Kai Johnsson

Publication

Lukinavicius et al Curr Biol. 2013 Feb 4;23(3):265-70. doi:

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SNAP-FGFR

Tags

FLAG-tag / His-tag

Expression

Mammalian

Promoter

CMV-TetO2

Available since

March 25, 2020

Availability

Academic Institutions and Nonprofits only

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pGEX-6P-1-INTS3-N

Plasmid

#128416

Purpose

Express INTS3 N-termini (1-513 aa) in E. coli with a GST tag (N-terminal)

Depositor

Yuliang Wu

Publication

Vidhyasagar et al Biochem J. 2018 Jan 2;475(1):45-60. doi:

Insert

INTS3 (INTS3 Human)

Tags

GST

Expression

Bacterial

Mutation

N-terminal region (1–513 aa)

Promoter

tac promoter

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

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A3H HapII-Cas9n-UGI

Plasmid

#119141

Purpose

Base editor made from APOBEC3H Haplotype II

Depositor

Reuben Harris

Publication

Martin et al Sci Rep. 2019 Jan 24;9(1):497. doi: 10.1

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

APOBEC3H Haplotype II-Cas9 nickase-UGI

Use

CRISPR

Mutation

None

Promoter

CMV

Available since

June 10, 2019

Availability

Academic Institutions and Nonprofits only

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pMCh2007_pLenti_Syn_mChe-cdc42E7

Plasmid

#118620

Purpose

expression of mChe-tagged cdc42E7 under pSyn promoter for primary neurons

Depositor

Marina Chekulaeva

Publication

Ciolli Mattioli et al Nucleic Acids Res. 2018 Dec 22. pii: 525

Insert

cdc42E7 (Cdc42 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

changed cagtctg to ggcataa (667 - 673 nt in 3…

Promoter

synapsin I (rat)

Available since

Jan. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pNTK Gatad2a flox

Plasmid

#110812

Purpose

Donor targeting vector for generating mouse Gatad2a conditional knockout allele

Depositor

Jacob Hanna

Publication

Mor et al Cell Stem Cell. 2018 Sep 6;23(3):412-425

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

mGataD2a (Gatad2a Mouse)

Use

Unspecified

Available since

Dec. 20, 2018

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup93-SpeI

Plasmid

#87331

Purpose

Expression of shRNA targeting at Nup93

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup93

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

June 7, 2017

Availability

Academic Institutions and Nonprofits only

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pSUPER-retro-puro-shNup35-EcoRI

Plasmid

#87332

Purpose

To express shRNA against human Nup35

Depositor

Lei Lu

Publication

Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

Insert

shRNA against human Nup35

Use

RNAi

Expression

Mammalian

Promoter

H1

Available since

May 1, 2017

Availability

Academic Institutions and Nonprofits only

Cited by

3 Articles

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MO70-hJunctate - EF-mutant

Plasmid

#79597

Purpose

Transient and retroviral expression

Depositor

Yousang Gwack

Publication

Srikanth et al Proc Natl Acad Sci U S A. 2012 May 29;10

Insert

junctate (ASPH Human)

Use

Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

77DAD79 to 77AAA79

Promoter

CMV

Available since

Aug. 17, 2016

Availability

Academic Institutions and Nonprofits only
pCMV5-Flag-Ago2-FDM4

Plasmid

#72211

Purpose

Mammalian expression of aa 373-860 of human Ago2

Depositor

Mien-Chie Hung

Publication

Shen et al Nature. 2013 May 16;497(7449):383-7. doi

Insert

argonaute RISC catalytic component 2 (AGO2 Human)

Tags

FLAG

Expression

Mammalian

Mutation

Δ 1-372

Promoter

CMV

Available since

May 4, 2016

Availability

Academic Institutions and Nonprofits only
GFP SDPR

Plasmid

#68398

Purpose

Expression of fluorescently tagged SDPR in mammalian cells

Depositor

Rob Parton

Publication

Bastiani et al J Cell Biol. 2009 Jun 29;185(7):1259-73.

Has service

50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA

Insert

SDPR (Sdpr Mouse)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available since

Sept. 24, 2015

Availability

Academic Institutions and Nonprofits only
pET41s-GST-ATF6a-AD

Plasmid

#64774

Purpose

bacterial expression of GST-tagged ATF6 (1-150)

Depositor

Joan Conaway, Ronald Conaway

Publication

Sela et al J Biol Chem. 2012 Jun 29;287(27):23035-4

Insert

ATF6a-AD (1-150) (ATF6 Human)

Tags

GST

Expression

Mammalian

Mutation

C-term deletion

Available since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Publication

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Tags

SNAPf and TwinStrepII

Expression

Insect

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

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pHL 1405

Plasmid

#53010

Purpose

pLtetO-1:RhyB, pCon:DsrA, pLtetO-1:hfq, pLlacO-1:sodB::gfp

Depositor

Han Lim

Publication

Hussein et al Proc Natl Acad Sci U S A. 2011 Jan 18;10

Inserts

RhyB sRNA
DsrA sRNA
hfq
sodB

Use

Synthetic Biology

Tags

gfp

Expression

Bacterial

Mutation

contains -56 to +141 relative to start codon

Promoter

pCon, pLlacO-1, and pLtetO-1

Available since

May 16, 2014

Availability

Academic Institutions and Nonprofits only