59,543 results

1. pSNAPf-hYTHDF1

   Plasmid

   #155346

   Purpose

   Express full-length human YTHDF1 with a C-terminal SNAP-tag in mammalian cells

   Depositor

   Xiaowei Zhuang

   Article

   Fu et al Nat Chem Biol. 2020 May 25. pii: 10.1038

   Insert

   YTHDF1 (YTHDF1 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. mScarlet-Rab9a

   Plasmid

   #169074

   Purpose

   Express human Rab9a in mammalian cells

   Depositor

   Gia Voeltz

   Article

   Wu et al Dev Cell. 2021 Jan 11;56(1):52-66.e7. do

   Insert

   Rab9a (RAB9A Human)
   

   Tags

   mScarlet

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 6, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Flag-HA-USP10

   Plasmid

   #22543

   Depositor

   Wade Harper

   Article

   Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

   Insert

   USP10 (USP10 Human)
   

   Use

   Retroviral

   Tags

   FLAG and HA

   Expression

   Mammalian

   Mutation

   None

   Available Since

   Jan. 20, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGW1-Myc-DJ1-WT

   Plasmid

   #29347

   Depositor

   Mark Cookson

   Article

   Miller et al J Biol Chem. 2003 Sep 19. 278(38):36588-

   Insert

   DJ1 (PARK7 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Available Since

   April 1, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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5. mCherry-Ykt6

   Plasmid

   #209888

   Purpose

   Expresses mCherry tagged Ykt6 in mammalian cells

   Depositor

   Geert van den Bogaart

   Insert

   Ykt6
   

   Tags

   mCherry

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 8, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. p4458 FLAG-hPLIC-1

   Plasmid

   #8663

   Depositor

   Peter Howley

   Article

   Kleijnen et al Mol Cell 2000 Aug;6(2):409-19.

   Insert

   hPLIC-1 (UBQLN1 Human)
   

   Tags

   FLAG

   Expression

   Mammalian

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

7. Flag-FOXF1

   Plasmid

   #153122

   Purpose

   Expression of Flag tagged human FOXF1 in mammalian cells

   Depositor

   Stefan Koch

   Article

   Moparthi et al Differentiation. 2020 Sep - Oct;115:30-3

   Insert

   human FOXF1 (FOXF1 Human)
   

   Tags

   Flag

   Expression

   Mammalian

   Promoter

   CMV IE94

   Available Since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. AICSDP-19:LAMP1-mEGFP

   Plasmid

   #101782

   Purpose

   Homology arms and linker-mEGFP sequence for C-terminus tagging of human LAMP1

   Depositor

   Allen Institute for Cell Science

   Insert

   LAMP1 Homology Arms with linker-mEGFP (LAMP1 Human)
   

   Use

   CRISPR; Donor template

   Tags

   linker-mEGFP

   Expression

   Mammalian

   Available Since

   Nov. 15, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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9. pEGFP-C1-PKM2

   Plasmid

   #64698

   Purpose

   Expression of GFP tagged PKM2 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

   Insert

   PKM2 (PKM Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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10. pWZL Neo Myr Flag PLK1

    Plasmid

    #20589

    Depositor

    William Hahn, Jean Zhao

    Article

    Boehm et al Cell. 2007 Jun 15. 129(6):1065-1079.

    Insert

    PLK1 (PLK1 Human)
    

    Use

    Retroviral

    Tags

    Flag and Myr

    Expression

    Mammalian

    Available Since

    Oct. 27, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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11. qTAG-C-Solo-mStayGold-VIM

    Plasmid

    #227302

    Purpose

    Donor template for mStayGold insertion into the C-terminus of the VIM locus. For intermediate filament visualization. To be co-transfected with sgRNA plasmid px330-PITCh-VIM (Addgene #227301)

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    VIM Homology Arms flanking a mStayGold Tag (VIM Human)
    

    Use

    CRISPR; Donor template

    Expression

    Mammalian

    Promoter

    Promoterless

    Available Since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pX330-PITCh-VIM

    Plasmid

    #227301

    Purpose

    Expresses SpCas9, the PITCh gRNA, and a sgRNA targeting the C-terminus of VIM for knock-in.

    Depositor

    Laurence Pelletier

    Article

    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

    Insert

    sgRNA Targeting C-terminus of VIM (VIM Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Nov. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pFRT/TO/HIS/FLAG/HA-ALKBH5

    Plasmid

    #38073

    Depositor

    Markus Landthaler

    Article

    Baltz et al Mol Cell. 2012 Jun 8;46(5):674-90.

    Insert

    ALKBH5 alkB, alkylation repair homolog 5 (E. coli) (ALKBH5 Human)
    

    Tags

    HIS/FLAG/HA

    Expression

    Mammalian

    Mutation

    cDNA clone MGC:71143 IMAGE:6339184 with longer 3&…

    Available Since

    Sept. 17, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. CMV Flag ATRwt

    Plasmid

    #41909

    Depositor

    Stephen Elledge

    Article

    Cortez et al Science. 2001 Nov 23;294(5547):1713-6.

    Insert

    ATR ATR serine/threonine kinase (ATR Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Promoter

    SRa

    Available Since

    Nov. 27, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. MEK1-E102-I103del-V5

    Plasmid

    #217301

    Purpose

    Lentivirus vector for expression of MEK1 E102-I103deletion

    Depositor

    Channing Der

    Insert

    MAP2K1 (MAP2K1 Human)
    

    Use

    Lentiviral

    Tags

    V5

    Mutation

    E102-I103deletion

    Available Since

    April 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pUltra-Hot-PC

    Plasmid

    #184466

    Purpose

    Lentiviral vector for bi-cistronic expression of mCherry and PC (seperated by P2A)

    Depositor

    Gerardo Ferbeyre

    Article

    Igelmann et al Mol Cell. 2021 Sep 16;81(18):3848-3865.e

    Insert

    Pyruvate Carboxylase (PC Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    UbC

    Available Since

    Aug. 1, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pEYFP-Sec24C

    Plasmid

    #66608

    Purpose

    YFP fusion of Sec24C

    Depositor

    David Stephens

    Article

    Stephens et al J Cell Sci. 2000 Jun;113 ( Pt 12):2177-8

    Insert

    Sec24C (SEC24C Human)
    

    Expression

    Mammalian

    Available Since

    July 27, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. pdCas9-DNMT3A-PuroR

    Plasmid

    #71667

    Purpose

    Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

    Depositor

    Vlatka Zoldos

    Article

    Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

    Insert

    S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A Human, S. pyogenes, Synthetic)
    

    Use

    CRISPR

    Tags

    3xFLAG, SV40 NLS, and T2A-PuroR

    Expression

    Mammalian

    Mutation

    D10A and H840A in S.pyogenes Cas9

    Promoter

    CBh

    Available Since

    March 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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19. TFORF0978

    Plasmid

    #141775

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    KLF7 (KLF7 Human)
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCSIIpuro-EREG-ScNeo

    Plasmid

    #209896

    Purpose

    To monitor the status of Epiregulin, the plasmid encodes a recombinant Epiregulin fused extracellularly to the mScarlet fluorescent protein and intracellularly to the mNeonGreen fluorescent protein

    Depositor

    Michiyuki Matsuda

    Article

    Deguchi et al Cell Rep. 2024 Nov 14;43(11):114986. doi

    Insert

    EREG-ScNeo (EREG Human)
    

    Use

    Lentiviral

    Tags

    GGGSGGGS linker, HA tag, mNeonGreen, and mScarlet…

    Available Since

    Nov. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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