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pME-24xMBS

Plasmid

#86246

Purpose

Middle entry vector for generating MBS tagged RNAs

Depositor

Florence Marlow

Article

Campbell et al Development. 2015 Apr 1;142(7):1368-74.

Type

Empty backbone

Use

Unspecified

Tags

NLS-tdMCP

Available Since

Jan. 26, 2017

Availability

Academic Institutions and Nonprofits only

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oxBFP

Plasmid

#68063

Purpose

C-terminal fusion to oxBFP

Depositor

Erik Snapp

Article

Costantini et al Nat Commun. 2015 Jul 9;6:7670. doi: 10.1

Type

Empty backbone

Tags

oxBFP

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 12, 2015

Availability

Academic Institutions and Nonprofits only
pcrRNA.con

Plasmid

#61285

Purpose

Constitutive repeat-only E. coli CRISPR array

Depositor

Chase Beisel

Article

Luo et al Nucleic Acids Res. 2014 Oct 17. pii: gku

Type

Empty backbone

Use

CRISPR

Expression

Bacterial

Promoter

J23119

Available Since

Feb. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pHygO2

Plasmid

#200077

Purpose

hygR selection unmarked rapid RMCE integration vector

Depositor

Michael Nonet

Article

Nonet et al Genetics. 2023 Apr 20:iyad072. doi: 10.1

Type

Empty backbone

Use

Recombination mediated cassette exchange

Expression

Worm

Available Since

May 19, 2023

Availability

Academic Institutions and Nonprofits only

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MIGS2.1 (FF570)

Plasmid

#35247

Purpose

Expression of miR173 precursor under UBQ10 promoter; includes miR173 target site after 35S promoter (Basta selection marker)

Depositor

Detlef Weigel

Article

de Felippes et al Plant J. 2011 Dec 28. doi: 10.1111/j.136

Type

Empty backbone

Expression

Plant

Promoter

CaMV 35S

Available Since

March 23, 2012

Availability

Academic Institutions and Nonprofits only

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pHAT4

Plasmid

#112585

Purpose

Used for expression of protein in E. coli as Thrombin and TEV cleavable N-terminal 6His-tag fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His

Expression

Bacterial

Available Since

Aug. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pMG10

Plasmid

#31238

Depositor

Keith Joung

Article

Soellu et al Nucleic Acids Res. 2010 Dec 1. 38(22):82

Type

Empty backbone

Tags

3xFLAG

Expression

Mammalian

Available Since

Aug. 1, 2011

Availability

Academic Institutions and Nonprofits only

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pIB167

Plasmid

#90190

Purpose

E. coli - Streptococci shuttle plasmid for gene expression in streptococci with Pspac promoter

Depositor

Indranil Biswas

Article

Biswas et al Microbiology. 2008 Aug;154(Pt 8):2275-82

Type

Empty backbone

Tags

His-tag and Strep-tag

Expression

Bacterial

Available Since

April 18, 2018

Availability

Academic Institutions and Nonprofits only

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DinoIII-gfp

Plasmid

#128141

Purpose

Dinoflagelate backbone for gfp expression

Depositor

Senjie Lin

Article

Sprecher et al Microorganisms. 2020 Jan 16;8(1). pii: m

Insert

Green fluorescence protein

Expression

Bacterial

Promoter

Dinoflagellate potential promoters

Available Since

April 29, 2020

Availability

Academic Institutions and Nonprofits only

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pGAT2

Plasmid

#112588

Purpose

Used for expression of protein in E. coli as Thrombin cleavable N-terminal 6His-tag-GST fusions

Depositor

Marko Hyvönen, Johan Peränen

Article

Peranen et al Anal Biochem. 1996 May 1;236(2):371-3.

Type

Empty backbone

Tags

6His-GST

Expression

Bacterial

Available Since

Aug. 27, 2018

Availability

Academic Institutions and Nonprofits only

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pAID5.2-C

Plasmid

#145811

Purpose

An all-in-one bicistronic plasmid for controlling protein degradation by AID technology

Depositor

Masato Kanemaki

Article

Yesbolatova et al Pharmaceuticals (Basel). 2020 May 23;13(

Type

Empty backbone

Tags

mAID-EGFP

Expression

Mammalian

Available Since

June 2, 2020

Availability

Academic Institutions and Nonprofits only

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pLO86

Plasmid

#91724

Purpose

mycobacterial expression of mCherry reporter

Depositor

Graham Hatfull

Article

Oldfield et al J Bacteriol. 2014 Oct;196(20):3589-97. d

Type

Empty backbone

Use

Unspecified; Mycobacterial expression

Tags

mCherry

Available Since

May 30, 2017

Availability

Academic Institutions and Nonprofits only

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pNIC-NHSTIIT-AVITAG-GG

Plasmid

#215802

Purpose

Cloning of target gene with N terminal His6 Twin Strep tag and TEV cleavage non cleavable N-terminal AVITAG

Depositor

ASAP Discovery, Frank von Delft

Article

Fairhead et al bioRxiv 2024.02.13.579886

Type

Empty backbone

Expression

Bacterial

Available Since

April 25, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pJH110

Plasmid

#68376

Purpose

Clone cassette to replace RFP for integration into C31 landing pad strain

Depositor

Trevor Charles

Article

Heil et al J Vis Exp. 2012 Mar 16;(61). pii: 3698.

Type

Empty backbone

Available Since

Oct. 26, 2015

Availability

Academic Institutions and Nonprofits only

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pFA6a-G11-5FLAG-kanMX6

Plasmid

#49182

Purpose

genomic G11-5FLAG epitope tagging in S. pombe

Depositor

Eishi Noguchi

Article

Gadaleta et al Biotechniques. 2013 Nov;55(5):257-63. do

Type

Empty backbone

Tags

G11-5FLAG

Expression

Yeast

Available Since

Nov. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAL250

Plasmid

#201672

Purpose

Contains 250 bp region of Pxyl promoter; insensitive to xylose.

Depositor

Alexandre Bisson

Article

Rados et al Front Microbiol. 2023 Aug 10;14:1204876.

Type

Empty backbone

Use

Expression in haloarchaea

Promoter

Pfdx, Pxyl

Available Since

May 31, 2023

Availability

Academic Institutions and Nonprofits only

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pDS1904

Plasmid

#175596

Purpose

Integrative vector for Candida albicans with dominant SAT1 marker

Depositor

Dominique Sanglard

Article

Dorsaz et al Front Microbiol. 2017 Aug 3;8:1478. doi:

Type

Empty backbone

Available Since

Nov. 2, 2021

Availability

Academic Institutions and Nonprofits only

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pAAVS1_Puro_ccdb_Ubc_rtTA

Plasmid

#172130

Purpose

Destination vector for AAVS1 targeting

Depositor

Kenji Kontani

Article

Araki et al J Biol Chem. 2021 Nov 18:101428. doi: 10

Type

Empty backbone

Expression

Mammalian

Promoter

PGK1

Available Since

Nov. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pDHK29

Plasmid

#80783

Purpose

Cloning vector compatible with ColE1 plasmids with high copy number

Depositor

Gregory Phillips

Article

Phillips et al Biotechniques. 2000 Mar;28(3):400-2, 404

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 3, 2017

Availability

Academic Institutions and Nonprofits only

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pMApra-Ptol

Plasmid

#218359

Purpose

Empty vector with XylS-Pm expression system

Depositor

Michael Gebhardt

Article

Intorcia et al Appl Environ Microbiol. 2024 Sep 18;90(9

Type

Empty backbone

Expression

Bacterial

Available Since

May 22, 2024

Availability

Academic Institutions and Nonprofits only

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pUG-TP3

Plasmid

#104651

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-TP4

Plasmid

#104652

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-TP5

Plasmid

#104653

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-41308

Plasmid

#104641

Purpose

Level 0 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-1287

Plasmid

#104642

Purpose

Level 0 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pUG-TP1

Plasmid

#104649

Purpose

Level 1 cloning vector

Depositor

Marco Trujillo

Article

Kowarschik et al New Phytol. 2018 Mar;217(4):1749-1763. d

Type

Empty backbone

Use

Synthetic Biology

Available Since

Feb. 1, 2019

Availability

Academic Institutions and Nonprofits only

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pSEVAT7Z1A

Plasmid

#58300

Purpose

GeneGuard vector cassette: thyA, ColE2 COR, Zeta toxin; with ampR as cargo.

Depositor

Tom Ellis

Article

Wright et al ACS Synth Biol. 2014 May 13.

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Available Since

Aug. 26, 2014

Availability

Academic Institutions and Nonprofits only

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pEx_MCS-attBtagRFPt

Plasmid

#48876

Purpose

PhiC31 exchange vector with multiple cloning site, exchange of EGFP in landing site with tagRFPt

Depositor

Joachim Wittbrodt

Article

Kirchmaier et al Development. 2013 Sep 18.

Type

Empty backbone

Use

Unspecified

Available Since

Dec. 11, 2013

Availability

Academic Institutions and Nonprofits only

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pEDJ400

Plasmid

#177269

Purpose

USER cloning-compatible 2μ vector (URA3)

Depositor

Michael Jensen

Article

Jensen et al Nucleic Acids Res. 2021 Sep 7;49(15):e88

Type

Empty backbone

Expression

Bacterial and Yeast

Available Since

Jan. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pNR-DEST

Plasmid

#90101

Purpose

Gateway destination vector featuring nitrate reductase (NR) promoter in front of AttR1/2 sites on episomal vector maintained in the diatom Phaeodactylum tricornutum.

Depositor

Philip Weyman

Article

Bielinski et al PeerJ Preprints. 2017 5:e2907v1

Type

Empty backbone

Use

Gateway destination vector for protein expression…

Available Since

June 26, 2017

Availability

Academic Institutions and Nonprofits only

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