Skip to main content
Addgene
Showing: 6541 - 6560 of 77368 results

  1. pGBW-m4046546

    Plasmid
    #145384
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF3a protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF3a protein (ORF3a Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;StrepII
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145384-plasmid-map-sequence-id-287561

    Enlarge

  2. pGBW-m4046507

    Plasmid
    #145385
    Purpose
    Yeast Expression plasmid for SARS-CoV-2 ORF10 protein
    Depositor
    Benjie Chen, Ginkgo Bioworks
    Insert
    SARS-CoV-2 ORF10 protein (ORF10 Severe acute respiratory syndrome coronavirus 2, Budding Yeast)
    Use
    Tags
    Cleavable thrombin;6xHIS
    Expression
    Yeast
    Mutation
    Saccharomyces cerevisiae recode 1
    Promoter
    pTEF1
    Available since
    April 29, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    145385-plasmid-map-sequence-id-287562

    Enlarge

  12. pET-sCS4T

    Plasmid
    #28249
    Depositor
    Yawen Bai
    Article
    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
    Insert
    single chain Cse4-Scm3-H4 with Thrombin site
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A thrombin cutting site between Cse4 and Scm3
    Promoter
    Available since
    July 12, 2011
    Availability
    Academic Institutions and Nonprofits only

  14. pET-sC4T

    Plasmid
    #28251
    Depositor
    Yawen Bai
    Article
    Zhou et al Nature. 2011 Apr 14. 472(7342):234-7.
    Insert
    single chain Cse4-H4 with Thrombin site
    Use
    Tags
    His
    Expression
    Bacterial
    Mutation
    A thrombin cutting site between Cse4 and H4
    Promoter
    Available since
    July 11, 2011
    Availability
    Academic Institutions and Nonprofits only

  17. L284F Cus2p

    Plasmid
    #73059
    Purpose
    S. cerevisiae Cus2p gene with L284F Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Leucine 284 to Phenylalanine
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    73059-plasmid-map-sequence-id-150795

    Enlarge

  18. D282N Cus2p

    Plasmid
    #73060
    Purpose
    S. cerevisiae Cus2p gene with D282N Mutation
    Depositor
    Aaron Hoskins
    Article
    Rodgers et al RNA. 2015 Dec 2.
    Insert
    CUS2p (CUS2 Budding Yeast)
    Use
    Tags
    6x His tag fused to TEV cleavage site
    Expression
    Bacterial and Yeast
    Mutation
    Asp 282 to Asn
    Promoter
    T7
    Available since
    Availability
    Academic Institutions and Nonprofits only
    73060-plasmid-map-sequence-id-150798

    Enlarge

  19. lentiCRISPR v2

    Plasmid
    #52961
    Purpose
    Replaces original lentiCRISPRv1 (Addgene Plasmid 49535) and produces ~10-fold higher titer virus. 3rd generation lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Sanjana et al Nat Methods. 2014 Aug;11(8):783-4. doi:
    Inserts
    Cas9
    Puromycin resistance
    Use
    CRISPR and Lentiviral
    Tags
    FLAG
    Expression
    Mammalian
    Mutation
    Promoter
    EFS-NS
    Available since
    May 8, 2014
    Availability
    Academic Institutions and Nonprofits only
    52961-plasmid-map-sequence-id-244694

    Enlarge

Showing: 6541 - 6560 of 77368 results