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p663-UBC-miniTurbo-V5-NRF2

Plasmid

#207811

Purpose

N-terminal tagged NRF2 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF2 (NFE2L2 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF2-V5-miniTurbo

Plasmid

#207812

Purpose

C-terminal tagged NRF2 for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF2-V5-miniTurbo (NFE2L2 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

Promoter

UBC

Available since

Dec. 12, 2023

Availability

Academic Institutions and Nonprofits only

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pET15b-His-3C-irisin

Plasmid

#122612

Purpose

Expresses in E. coli: human irisin with Nt histag and 3C protease site for cleavage

Depositor

Harold Erickson

Article

Albrecht et al Sci Rep. 2015 Mar 9;5:8889. doi: 10.1038

Insert

irisin (ectodomain of FNDC5) (Fndc5 Rat, Human, Mouse, Bovine)

Use

Tags

Nt: histag-3C protease site

Expression

Bacterial

Mutation

Promoter

T7 polymerase

Available since

March 26, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO K164A H165A CoV-2 nsp1 3xFLAG

Plasmid

#176060

Purpose

Expresses K164A K165A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 K164A H165A nsp1 (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

K164A and H165A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA4TO R124A K125A CoV-2 nsp1 3xFLAG

Plasmid

#176059

Purpose

Expressing R124A K125A CoV2 nsp1 in mammalian cells. This contains a C-terminal 3xFLAG tag fusion to nsp1.

Depositor

Britt Glaunsinger

Article

Mendez et al Cell Rep. 2021 Oct 19;37(3):109841. doi:

Insert

CoV-2 nsp1 R124A/K125A (ORF1ab SARS-CoV-2)

Use

Tags

3xFLAG

Expression

Mammalian

Mutation

R124A and K125A

Promoter

CMV

Available since

Oct. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 WT

Plasmid

#69819

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_01

Plasmid

#158102

Purpose

5'UTR stem-loop luciferase reporter: 0kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop 0kcal/mol

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pJWK_VD_02

Plasmid

#158103

Purpose

5'UTR stem-loop luciferase reporter: -30kcal/mol

Depositor

Ted Dawson

Article

Kim et al Cell Stem Cell. 2020 Oct 1;27(4):633-645

Insert

5'UTR synthetic stem-loop -30kcal/mol

Use

Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

PGK

Available since

Aug. 18, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.2/V5-DEST CDC42EP1 S192A

Plasmid

#69820

Purpose

Expresses CDC42EP1-V5 in mammalian cells

Depositor

Anne Brunet

Article

Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

Insert

CDC42 effector protein (CDC42EP1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

S192A

Promoter

CMV

Available since

April 22, 2016

Availability

Academic Institutions and Nonprofits only

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pOP795

Plasmid

#235700

Purpose

Protein expression His-taged HsNRBF2_FL in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 (NRBF2 Human)

Use

Tags

His6(TEV)

Expression

Bacterial

Mutation

Promoter

Available since

April 28, 2025

Availability

Academic Institutions and Nonprofits only

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pOP796

Plasmid

#235701

Purpose

Protein expression of His-tagged HsNRBF2 MIT domain in bacteria

Depositor

Roger Williams

Article

Ohashi et al Autophagy. 2016 Nov;12(11):2129-2144. do

Insert

NRBF2 MIT domain (NRBF2 Human)

Use

Tags

His6(TEV)

Expression

Bacterial

Mutation

aa 1-89

Promoter

Available since

April 16, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-DDX3_helicase_core_Q360L

Plasmid

#233619

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360L mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Leu (DDX3X Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Gln360Leu

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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pColdI-DDX3_helicase_core_Q360P

Plasmid

#233620

Purpose

Expression of His-tagged helicase core region of DDX3X with Q360P mutation in E.coli

Depositor

Shintaro Iwasaki

Article

Chen et al Cell Chem Biol. 2021 Apr 15;28(4):475-48

Insert

DDX3X helicase core Gln360Pro (DDX3X Human)

Use

Tags

His6

Expression

Bacterial

Mutation

Gln360Pro

Promoter

Available since

March 11, 2025

Availability

Academic Institutions and Nonprofits only

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2C-EGFP-t2a-mCD4

Plasmid

#216213

Purpose

Expresses GFP and mCD4 transmembrane domain under control of the 2C-specific MERVL promoter

Depositor

Michelle Percharde

Article

Xie et al Genes Dev. 2022 Mar 1;36(5-6):331-347. d

Insert

CD4 (Cd4 Mouse)

Use

Tags

GFP-T2A

Expression

Mammalian

Mutation

CD4 transmembrane domain only

Promoter

MERVL

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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p663-UBC-miniTurbo-V5-NRF2-E79Q

Plasmid

#207813

Purpose

N-terminal tagged NRF2-E79Q for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

miniTurbo-V5-NRF2-E79Q (NFE2L2 Human)

Use

Lentiviral

Tags

miniTurbo-V5

Expression

Mutation

E79Q

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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p667-UBC-NRF2-E79Q-V5-miniTurbo

Plasmid

#207814

Purpose

C-terminal tagged NRF2-E79Q for miniTurbo proximity experiments

Depositor

Ben Major

Article

LaPak et al Sci Signal. 2023 Dec 12;16(815):eadi9018

Insert

NRF2-E79Q-V5-miniTurbo (NFE2L2 Human)

Use

Lentiviral

Tags

V5-miniTurbo

Expression

Mutation

E79Q

Promoter

UBC

Available since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Xenopus (Silurana) tropicalis

Plasmid

#75355

Purpose

Nt His-tag Xenopus (Silurana) tropicalis Sacsin 87-1376

Depositor

Kalle Gehring

Insert

Sacsin Xenopus (Silurana) tropicalis 87-1376

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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pUPD2 nickase D10A Cas9 (GB1690)

Plasmid

#160587

Purpose

Nickase D10A Cas9 for Nt and Ct fusion.

Depositor

Diego Orzaez

Insert

nickase D10A Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pUPD2 nickase D10A Cas9 (GB1691)

Plasmid

#160588

Purpose

Nickase D10A Cas9 for Nt fusion.

Depositor

Diego Orzaez

Insert

nickase D10A Cas9

Use

CRISPR and Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pColdII-SIRPT1-Ceratotherium-simum-simum

Plasmid

#78111

Purpose

Nt His-tag Ceratotherium-simum-simum Sacsin 89-1366

Depositor

Kalle Gehring

Insert

Sacsin Ceratotherium-simum-simum 89-1366

Use

Tags

His-Tag

Expression

Bacterial

Mutation

Promoter

Available since

July 11, 2016

Availability

Academic Institutions and Nonprofits only

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