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Plasmid#65812Purposeexpression of eIF4G-MS2 fusion.DepositorInserteIF4G (EIF4G1 Human)
UseTagsMS2 stem loops and V5ExpressionMammalianMutationdelN83 (functionally behaves as the WT protein)PromoterCMVAvailable sinceSept. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
PB-iRFP-STOP-ReNL
Plasmid#113965PurposePiggybac transposon plasmid CRISPR gene deletion activatable fluorescence. Constitutive iRFP670 under EF1A promoter, CMV promoter with two SV40 polyA followed by red-enhanced nanolantern (ReNL)DepositorInsertsiRFP670
ReNL
UsePiggybac transposonTagsExpressionMammalianMutationPromoterCMV - SV40-PolyA - SV40-PolyA and EF1AAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
ABEmax-Puro V2
Plasmid#226955PurposeABEmax plasmid enabling A:T to G:C base editing using a single plasmid.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceNov. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 GFP V3
Plasmid#226961PurposeCBh-SaCas9-2A-GFP, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
PDG459 V3
Plasmid#226958PurposeCBh-SpCas9-2A-Puro, and 2X hU6-sgRNA (Sp) with BbsI golden gate cloning backbone dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
SaCas9 GFP V3
Plasmid#226962PurposeCBh-SaCas9-2A-GFP, and hU6-sgRNA (Sa) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Pdg459-eSp(1.1) V3
Plasmid#226965PurposeCBh-eSpCas9(1.1)-2A-Puro, and 2X hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
DG SaCas9 puro V3
Plasmid#226960PurposeCBh-SaCas9-2A-Puro, and 2X hU6-sgRNA (Sa) with BbsI golden gate cloning backbone for dual gRNAs. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
rUBR5_2383-2442_pGEX6P1
Plasmid#209791PurposeBacterial expression of UBR5 Mlle domainDepositorInsertMlle domain of UBR5 (Ubr5 Rat)
UseTagsGSTExpressionBacterialMutationaa 2383-2442 onlyPromotertacAvailable sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-509
Plasmid#209800PurposeBacterial expression of human LARP1 La-moduleDepositorInsertLARP1 La-module (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-509 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
LARP1_323-410
Plasmid#209801PurposeBacterial expression of human LARP1 LaM domainDepositorInsertLARP1 LaM (LARP1 Human)
UseTags6xHisExpressionBacterialMutationaa 323-410 onlyPromoterT7Available sinceNov. 10, 2023AvailabilityAcademic Institutions and Nonprofits only -
pUC19-U6-terminator (SL695)
Plasmid#133961PurposepUC19 plasmid with U6 promoter followed by a poly-T termination signalDepositorInsertU6 promoter
UseTagsExpressionMammalianMutationN/APromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
(P79) OCN/Wnt 10b transgene
Plasmid#25878DepositorInsertWnt10b (Wnt10b Mouse)
UseMouse TargetingTagsB-globin intron/poly A coding sequence and OCN pr…ExpressionMammalianMutationPromoterAvailable sinceOct. 21, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPE2iso4_Z
Plasmid#148119PurposeMammalian Expression of HshnRNPE2iso4DepositorInsertHshnRNPE2iso4 (PCBP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-MS2-HA-HshnRNPE1_Z
Plasmid#148115PurposeMammalian Expression of HshnRNPE1DepositorInsertHshnRNPE1 (PCBP1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceMarch 16, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT7-V5-SBP-C1-HshnRNPE2iso4_Z
Plasmid#148141PurposeMammalian Expression of HshnRNPE2iso4DepositorInsertHshnRNPE2iso4 (PCBP2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
PB-GFP-Gal8
Plasmid#127191PurposePiggybac transposon plasmid with CAG promoter GFP-Galectin 8 (GFP-Gal8) fusion protein. Useful as genetically encoded endosomal escape sensor.DepositorInsertGFP-Gal8 (LGALS8 Human)
UseTagsGal8 is fused to the c-terminus of GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
PX459v3
Plasmid#178799PurposeSpCas9 with 2A-Puro, and a golden gate cloning backbone for sgRNA. sgRNA scaffold seqeuence has been modified for increased sgRNA expression (v3).DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterCbh (Cas9-2A Puro) and U6 (gRNA)Available sinceAug. 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pEE075
Plasmid#176832PurposeEncodes codon optimized putative thermophilic PET hydrolase enzyme for bacterial expressionDepositorInsert714
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceNov. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLV-Hygro-EF1A-EGFP-PARP1
Plasmid#176146PurposeEGFP fused to the N-terminus of PARP1 & a hygromycin resistance cassetteDepositorInsertPoly(ADP-Ribose) Polymerase 1 (PARP1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationPromoterEF1AAvailable sinceJan. 27, 2022AvailabilityAcademic Institutions and Nonprofits only