1,878 results

1. pGC236

   Plasmid

   #19675

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)

   Use

   Flp/frt, destination vector

   Tags

   4xNLS

   Expression

   Worm

   Available Since

   Feb. 25, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. oma-2 ORF in L4440

   Plasmid

   #82548

   Purpose

   oma-2 RNAi feeding construct

   Depositor

   Sean Ryder

   Article

   Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

   Insert

   oma-2 ORF
   

   Expression

   Worm

   Available Since

   Dec. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

3. U653-GFP

   Plasmid

   #24477

   Depositor

   Ron Vale

   Article

   Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

   Insert

   Unc104 (unc-104 Nematode)
   

   Tags

   GFP

   Expression

   Bacterial

   Available Since

   April 23, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pEG587

   Plasmid

   #40196

   Depositor

   Geraldine Seydoux

   Article

   Griffin et al Cell. 2011 Sep 16;146(6):955-68.

   Insert

   Mex-5 (S458A) (RNAi resistant) (mex-5 Nematode)
   

   Tags

   Dendra2 PAFP (sequence optimized for worm express…

   Expression

   Worm

   Mutation

   S458A; exon 2 recoded to be RNAi-resistant

   Promoter

   4.4 kb mex-5 promoter fragment

   Available Since

   Sept. 19, 2012

   Availability

   Academic Institutions and Nonprofits only

5. pMALac-OMA-1(1-182)

   Plasmid

   #80350

   Purpose

   Expresses OMA-1 (1-182) in bacteria for protein purification purposes

   Depositor

   Sean Ryder

   Article

   Kaymak et al J Biol Chem. 2013 Oct 18;288(42):30463-7

   Insert

   OMA-1 (oma-1 Nematode)
   

   Tags

   6xHis-MBP-TEV

   Expression

   Bacterial

   Mutation

   Encodes AA residues 1-182

   Available Since

   Oct. 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pKPY738

   Plasmid

   #72674

   Purpose

   C. elegans let-858 3' UTR (Gateway Entry Clone)

   Depositor

   David Tirrell

   Insert

   let-858 3' UTR [From pPD129.57 (+2554 to +2960)] in pDONR P2R-P3
   

   Use

   Gateway entry clone

   Available Since

   Feb. 16, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pJW4.17

   Plasmid

   #62872

   Purpose

   Bacterial expression of MBP-MBK-2 K196R

   Depositor

   Geraldine Seydoux

   Article

   Wang et al Elife. 2014 Dec 23;4. doi: 10.7554/eLife

   Insert

   MBP-MBK-2 K196R
   

   Tags

   MBP

   Expression

   Bacterial

   Mutation

   K196R

   Available Since

   March 18, 2015

   Availability

   Academic Institutions and Nonprofits only

8. pGC276

   Plasmid

   #19637

   Depositor

   Jane Hubbard

   Article

   Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

   Insert

   Gateway(R1-R2)

   Use

   Flp/frt, destination vector

   Expression

   Worm

   Available Since

   March 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. RGS-6xHis-DRE-1-pcDNA3.1-

   Plasmid

   #52517

   Purpose

   expresses C. elegans DRE-1 in mammalian cells with RGS-6xHis tag at N-terminus

   Depositor

   Adam Antebi

   Article

   Horn et al Dev Cell. 2014 Mar 4. pii: S1534-5807(14

   Insert

   Dre-1 (dre-1 Nematode)
   

   Tags

   RGS-6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   July 31, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pPD95.75-cYAP-1

    Plasmid

    #66857

    Purpose

    Generate transgenic C.elegans with cYAP-1 fused to GFP

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

    Insert

    C.elegans YAP-1 (yap-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Mutation

    Serine104 is mutated to alanine

    Promoter

    C. elegans 5'UTR

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

11. pPD95.75-cYAP-1 S104A

    Plasmid

    #66858

    Purpose

    Generate transgenic C.elegans with cYAP1- fused to GFP, in which Serine 104 is mutated to alanine

    Depositor

    Yutaka Hata

    Article

    Iwasa et al Exp Cell Res. 2013 Apr 15;319(7):931-45.

    Insert

    YAP-1 (yap-1 Nematode)
    

    Tags

    GFP

    Expression

    Worm

    Mutation

    Serine104 is mutated to alanine

    Available Since

    Aug. 17, 2015

    Availability

    Academic Institutions and Nonprofits only

12. TU#1448_unc-73b_cDNA

    Plasmid

    #82362

    Purpose

    express unc-73b cDNA

    Depositor

    Martin Chalfie

    Article

    Zheng et al Proc Natl Acad Sci U S A. 2016 Jun 21;11

    Insert

    unc-73 b isoform (unc-73 Nematode)
    

    Expression

    Worm

    Promoter

    none

    Available Since

    Aug. 30, 2016

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pGC246

    Plasmid

    #19673

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    RNAi; Flp/frt, destination vector

    Expression

    Worm

    Available Since

    March 5, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pOX-twk41

    Plasmid

    #16127

    Depositor

    Larry Salkoff

    Article

    Salkoff et al Neuroscience. 2001 . 103(4):853-9.

    Insert

    Tandem pore domain K+ channel (egl-23 Nematode)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    May 13, 2008

    Availability

    Academic Institutions and Nonprofits only

15. pOX-unc103

    Plasmid

    #16213

    Depositor

    Larry Salkoff

    Insert

    eag-like K+ channels (unc-103 Nematode)
    

    Use

    Xenopus oocyte expression

    Mutation

    N/A

    Available Since

    Jan. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

16. U653-HTR

    Plasmid

    #24470

    Depositor

    Ron Vale

    Article

    Tomishige et al Science. 2002 Sep 27. 297(5590):2263-7.

    Insert

    Unc104 (unc-104 Nematode)
    

    Tags

    His

    Expression

    Bacterial

    Available Since

    June 2, 2010

    Availability

    Academic Institutions and Nonprofits only

17. pCM5.49

    Plasmid

    #21391

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    pal-1 3'utr in pDONRP2RP3
    

    Use

    Gateway

    Available Since

    July 1, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pSR21

    Plasmid

    #69156

    Purpose

    read-outlox2272 mCherry to tagBFP switch for integration on ttTi14024, Chr X

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    rps-27

    Available Since

    Nov. 2, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEG608

    Plasmid

    #40194

    Depositor

    Geraldine Seydoux

    Article

    Griffin et al Cell. 2011 Sep 16;146(6):955-68.

    Insert

    Mex-5 (RNAi resistant) (mex-5 Nematode)
    

    Tags

    3xFLAG (DYKDHDGDYKDHDIDYKDDDDK), GFP, and TEV pro…

    Expression

    Worm

    Mutation

    Exon 2 recoded to be RNAi-resistant

    Promoter

    4.4 kb mex-5 promoter fragment

    Available Since

    Sept. 19, 2012

    Availability

    Academic Institutions and Nonprofits only

20. pCM3.08

    Plasmid

    #19405

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    pie-1 ORF+3' in pDONR201 (pie-1 Nematode)
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

21. pGC91

    Plasmid

    #19621

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<4xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Expression

    Worm

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

22. pGC114

    Plasmid

    #19622

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Prpl-28<1xNLS-GFP::let-858(3’)-(Cbr)unc-119(+)<1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Expression

    Worm

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pGC231

    Plasmid

    #19644

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    Use

    Flp/frt

    Tags

    4xNLS

    Expression

    Worm

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pGC78

    Plasmid

    #19654

    Depositor

    Jane Hubbard

    Article

    Voutev et al Genetics. 2008 Sep;180(1):103-19. doi: 1

    Insert

    <1xNLS-lacZ::unc-54(3’)
    

    Use

    Flp/frt

    Tags

    1xNLS

    Expression

    Worm

    Available Since

    Feb. 23, 2009

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCM3.09

    Plasmid

    #19406

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    fbf-2 ORF+3' in pDONR201 (fbf-2 Nematode)
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pcDNAOX-twk5

    Plasmid

    #16105

    Depositor

    Larry Salkoff

    Article

    Wei et al Neuropharmacology. 1996 . 35(7):805-29.

    Insert

    Tandem pore domain K+ channel (twk-5 Nematode)
    

    Use

    Mammalian expression & oocyte expression

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

27. pOX-twk24

    Plasmid

    #16120

    Depositor

    Larry Salkoff

    Article

    Salkoff et al Neuroscience. 2001 . 103(4):853-9.

    Insert

    Tandem pore domain K+ channel (twk-24 Nematode)
    

    Use

    Xenopus oocyte expression

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

28. pCM5.35

    Plasmid

    #19395

    Depositor

    Geraldine Seydoux

    Article

    Merritt et al Curr Biol. 2008 Oct 14;18(19):1476-82. d

    Insert

    nos-3 3'utr in pDONRP2RP3 (nos-3 Nematode)
    

    Expression

    Worm

    Available Since

    Nov. 10, 2008

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. mg172

    Plasmid

    #34652

    Depositor

    Miriam Goodman

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2011 Nov 2.

    Insert

    slo-1(A1;B1;C0) (slo-1 Nematode)
    

    Use

    Xenopus oocyte expression

    Tags

    Xenopus beta-globulin 3' UTR and Xenopus bet…

    Available Since

    Feb. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pSR04

    Plasmid

    #69151

    Purpose

    read-outloxP mCherry to GFP switch, with eft-1::tagBFP::tbb-2UTR as gene of interest for integration on cxtTi10816, Mos Chr IV

    Depositor

    Sander van den Heuvel

    Article

    Ruijtenberg et al Cell. 2015 Jul 16;162(2):300-13. doi: 10

    Inserts

    mCherry
    TagBFP
    eGFP
    

    Use

    Cre/Lox; Mossci

    Expression

    Worm

    Mutation

    codon-optimzed index 1.0

    Promoter

    eef-1A.1 (eft-3) and rps-27

    Available Since

    Oct. 20, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge