We narrowed to 4,128 results for: pCMV mammalian

1. HA-Foxo1ADA (pCMV5)

   Plasmid

   #12143

   Depositor

   Domenico Accili

   Article

   Nakae et al J Clin Invest. 2001 Nov . 108(9):1359-67

   Insert

   Foxo1 ADA (Foxo1 Mouse)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Mutation

   Phosphorylation-deficient, also referred to as co…

   Promoter

   CMV

   Available Since

   Aug. 2, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCMVTNT-T7-KPNA1

   Plasmid

   #26677

   Depositor

   Bryce Paschal

   Article

   Kelley et al BMC Cell Biol. 2010 Aug 11;11:63. doi: 1

   Insert

   Karyopherin Alpha 1 (KPNA1 Human)
   

   Tags

   T7

   Expression

   Mammalian

   Mutation

   KPNA1 sequence contains a reported SNP at S73N

   Available Since

   Nov. 10, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6-AC 4-μHOM

   Plasmid

   #113619

   Purpose

   Reporter for Alt-EJ, variant of EJ7-GFP. Double strand breaks are induced with the 7a & 7h, or 7a & 7i sgRNAs, with CAS9. EJ using 4 nt of microhomology causes a deletion mutation and restores GFP

   Depositor

   Jeremy Stark

   Article

   Bhargava et al Nat Commun. 2018 Jun 27;9(1):2484. doi:

   Insert

   4-μHOM variant of EGFP
   

   Expression

   Mammalian

   Available Since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCMV-Cyclin D1

   Plasmid

   #19927

   Depositor

   Yue Xiong

   Article

   Watanabe et al Proc Natl Acad Sci U S A. 1998 Feb 17. 9

   Insert

   Cyclin D1 (CCND1 Human)
   

   Expression

   Mammalian

   Available Since

   April 1, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCMV5 alpha 1A AR

   Plasmid

   #45760

   Depositor

   Robert Lefkowitz

   Article

   Schwinn et al J Biol Chem. 1990 May 15;265(14):8183-9.

   Insert

   alpha 1A AR (Adra1a Rat)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 21, 2013

   Availability

   Academic Institutions and Nonprofits only

6. pCMV-HA-MyD88-FW/AA

   Plasmid

   #12289

   Depositor

   Bruce Beutler

   Article

   Jiang et al Proc Natl Acad Sci U S A. 2006 Jul 18. 1

   Insert

   MyD88 (MYD88 Human)
   

   Tags

   HA

   Expression

   Mammalian

   Mutation

   F285A/W286A

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

7. pCMV5 MEKK4alpha WT

   Plasmid

   #12187

   Depositor

   Gary Johnson

   Article

   Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

   Insert

   MEKK4 (Map3k4 Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Sept. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

8. pCMV5-ALK6-HA

   Plasmid

   #11769

   Depositor

   Jeff Wrana

   Article

   Macias-Silva et al J Biol Chem. 1998 Oct 2. 273(40):25628-3

   Insert

   ALK6 (Bmpr1b Mouse)
   

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Aug. 4, 2006

   Availability

   Industry, Academic Institutions, and Nonprofits

9. pCMV6M-Mst2

   Plasmid

   #84296

   Purpose

   Expresses Myc-tagged Mst2

   Depositor

   Jonathan Chernoff

   Article

   Rawat et al Curr Biol. 2016 Jun 20;26(12):1556-63. d

   Insert

   Mst2 (STK3 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 19, 2016

   Availability

   Academic Institutions and Nonprofits only

10. pCMV5 MEKK4alpha KD

    Plasmid

    #12188

    Depositor

    Gary Johnson

    Article

    Gerwins et al J Biol Chem. 1997 Mar 28. 272(13):8288-9

    Insert

    MEKK4 (Map3k4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Kinase dead: K1361M

    Available Since

    Sept. 22, 2006

    Availability

    Academic Institutions and Nonprofits only

11. pCMV-Nxph4-HA

    Plasmid

    #128102

    Purpose

    Express HA tagged Nxph4

    Depositor

    Huda Zoghbi

    Article

    Meng et al Elife. 2019 Sep 16;8. pii: 46773. doi: 1

    Insert

    Nxph4 (Nxph4 Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Sept. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV4-HA-NEMO

    Plasmid

    #27560

    Depositor

    Shao-Cong Sun

    Article

    Harhaj et al J Biol Chem. 1999 Aug 13. 274(33):22911-

    Insert

    NEMO (IKBKG Human)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    S8R

    Available Since

    Aug. 30, 2011

    Availability

    Academic Institutions and Nonprofits only

13. M69 pCMV pGL3 Luciferase

    Plasmid

    #17186

    Depositor

    Randall Moon

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mammalian

    Available Since

    May 18, 2009

    Availability

    Academic Institutions and Nonprofits only

14. pVH228-Tier1-OVanO2-PCMVmin2-SEAP

    Plasmid

    #169564

    Purpose

    Tier-1 vector encoding PVanO2-driven SEAP expression (OVanO2-PCMVmin-2-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    vanillic acid-controlled SEAP production
    

    Expression

    Mammalian

    Promoter

    VanO2-PCMVmin

    Available Since

    July 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. pVH265-Tier1-OTetO7-PCMVmin2-TS-SEAP

    Plasmid

    #169588

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression with a suppressor 5'UTR (OTetO7-PCMVmin-2-5'UTRTS-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin-2

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pVH255-Tier1-OTetO7-PCMVmin1-SEAP

    Plasmid

    #169587

    Purpose

    Tier-1 vector encoding PTetO7-driven SEAP expression (OTetO7-PCMVmin-1-SEAP-pA).

    Depositor

    Martin Fussenegger

    Article

    Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

    Insert

    tetracycline-controlled SEAP expression
    

    Expression

    Mammalian

    Promoter

    TetO7-PCMVmin-1

    Available Since

    June 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-SlugCas9-P2A-puro

    Plasmid

    #214353

    Purpose

    Expresses SlugCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SlugCas9 and puromycin resistance genes
    

    Expression

    Mammalian

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCMV-SpCas9-P2A-puro

    Plasmid

    #214358

    Purpose

    Expresses SpCas9 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 and puromycin resistance genes
    

    Expression

    Mammalian

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCMV-SPORT6-RNF114-RINGmut

    Plasmid

    #69922

    Purpose

    Expresses mutant myc-RNF114 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Expression

    Mammalian

    Mutation

    C29A, C32A

    Promoter

    CMV

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

20. pCMV-myc-hIFITM3-Y20A

    Plasmid

    #58462

    Purpose

    Expresses human IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 20 to alanine

    Promoter

    CMV IE

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

21. pCMV4a-SIRT6-H133Y-FLAG

    Plasmid

    #102324

    Purpose

    Human SIRT6 H133Y with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed histidine 133 to tyrosine

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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22. pCMV 3xFLAG-LC3B G120A

    Plasmid

    #123093

    Purpose

    Expresses 3xFLAG-LC3B G120A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    May 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

23. pCMV4a-SIRT6-G60A-FLAG

    Plasmid

    #102323

    Purpose

    Human SIRT6 G60A with FLAG tag for mammalian expression

    Depositor

    Hening Lin

    Article

    Zhang et al Nat Chem Biol. 2016 Aug;12(8):614-20. do

    Insert

    SIRT6 (SIRT6 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    changed glycine 60 to alanine

    Promoter

    CMV

    Available Since

    Oct. 12, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

24. pCMV-BE4-SsAPOBEC3B R54Q

    Plasmid

    #138344

    Purpose

    Mammalian expression plasmid for BE4-SsAPOBEC3B R54Q

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-SsAPOBEC3B R54Q
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

25. pCMV/3' Box_(GLuc)_INT

    Plasmid

    #68436

    Purpose

    Transient expression of an "INT" construct_bearing three PP7 Stem-loops, targeting the GLuc reporter, in mammalian cells. CMV/3' Box expression backbone.

    Depositor

    John Rinn

    Article

    Shechner et al Nat Methods. 2015 Jul;12(7):664-70. doi:

    Insert

    INT construct_bearing three PP7 Stem-loops
    

    Use

    CRISPR and Synthetic Biology

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

26. pCMV-BE4-RrA3F F130L

    Plasmid

    #138341

    Purpose

    Mammalian expression plasmid for BE4-RrA3F F130L

    Depositor

    Nicole Gaudelli

    Article

    Yu et al Nat Commun. 2020 Apr 28;11(1):2052. doi:

    Insert

    BE4-RrA3F F130L
    

    Use

    Synthetic Biology

    Tags

    BP-NLS

    Promoter

    CMV

    Available Since

    April 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

27. pCMV 3xFLAG-GABARAP G116

    Plasmid

    #123099

    Purpose

    Expresses 3xFLAG-GABARAP G116 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein (GABARAP Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acid 117. Stop codon after G116

    Promoter

    CMV

    Available Since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

28. pCMV 3xFLAG-LC3A G120A

    Plasmid

    #123090

    Purpose

    Expresses 3xFLAG-LC3A G120A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3A (MAP1LC3A Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 120 to Alanine

    Promoter

    CMV

    Available Since

    May 14, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

29. pCMV 3xFLAG-GABARAPL1 G116A

    Plasmid

    #123101

    Purpose

    Expresses 3xFLAG-GABARAPL1 G116A in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Gamma-aminobutyric acid receptor-associated protein-like 1 (GABARAPL1 Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Glycine 116 to Alanine

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

30. pCMV-Tag 3B/WT myc-M87

    Plasmid

    #87722

    Purpose

    Expresses WT myc-M87 spastin isoform in mammalian cells

    Depositor

    Peter Baas

    Article

    Solowska et al Hum Mol Genet. 2010 Jul 15;19(14):2767-7

    Insert

    SPAST (SPAST Human)
    

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    M87 isoform (deletion of nt 1-490 - 5'UTR an…

    Promoter

    CMV

    Available Since

    March 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

31. pCMV-SPORT6-RNF114-UIMmut

    Plasmid

    #69923

    Purpose

    Expresses mutant myc-RNF114 in mammalian cells

    Depositor

    Francesca Capon

    Article

    Bijlmakers et al Hum Mol Genet. 2011 Aug 15;20(16):3129-3

    Insert

    RNF114 (RNF114 Human)
    

    Expression

    Mammalian

    Mutation

    S224P

    Promoter

    CMV

    Available Since

    Feb. 25, 2016

    Availability

    Academic Institutions and Nonprofits only

32. pCMV 3xFLAG-LC3C G126

    Plasmid

    #123096

    Purpose

    Expresses 3xFLAG-LC3C G126 in mammalian cells.

    Depositor

    Robin Ketteler

    Article

    Agrotis et al Autophagy. 2019 Jan 21. doi: 10.1080/155

    Insert

    Microtubule-associated proteins 1A/1B light chain 3C (MAP1LC3C Human)
    

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Deleted amino acids 127-147. Stop codon after G12…

    Promoter

    CMV

    Available Since

    May 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

33. pCMV-myc-mIFITM3-deltaY20

    Plasmid

    #58459

    Purpose

    Expresses murine IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 20 deleted

    Promoter

    CMV IE

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

34. pCMV-myc-mIFITM3-Y20A

    Plasmid

    #58460

    Purpose

    Expresses murine IFITM3-Y20A with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 20 to alanine

    Promoter

    CMV IE

    Available Since

    Sept. 10, 2014

    Availability

    Academic Institutions and Nonprofits only

35. pCMV-SpCas9HF1-P2A-puro

    Plasmid

    #214357

    Purpose

    Expresses SpCas9HF1 and puromycin resistance genes

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9HF1 and puromycin resistance genes
    

    Expression

    Mammalian

    Mutation

    N497A/R661A/Q695A/Q926A

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

36. pCMV-mTagBFP2-FRB-Rab5

    Plasmid

    #214272

    Purpose

    Mammalian expression of mTagBFP2-FRB-Rab5 (early endosome marker)

    Depositor

    Shinya Tsukiji

    Article

    Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

    Insert

    mTagBFP2-FRB-Rab5A (RAB5A Canis lupus familiaris (Dog))
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

37. pCMV-miRFP670-eDHFR(69K6)

    Plasmid

    #178859

    Purpose

    Mammalian expression of miRFP670-eDHFR(69K6)

    Depositor

    Shinya Tsukiji

    Article

    Suzuki et al Cell Chem Biol. 2022 Sep 15;29(9):1446-1

    Insert

    miRFP670-eDHFR(69K6)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    July 14, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

38. pCMV-myc-mIFITM3-Y27A

    Plasmid

    #58464

    Purpose

    Expresses murine IFITM3-Y27A with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (Ifitm3 Mouse)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 27 to alanine

    Promoter

    CMV IE

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

39. pCMV-myc-hIFITM3-deltaY20

    Plasmid

    #58463

    Purpose

    Expresses human IFITM3-deltaY20 with an N-terminal myc tag in mammalian cells.

    Depositor

    Jacob Yount

    Article

    Chesarino et al J Biol Chem. 2014 Apr 25;289(17):11986-9

    Insert

    IFITM3 (IFITM3 Human)
    

    Tags

    myc

    Expression

    Mammalian

    Mutation

    Tyrosine 20 deleted

    Promoter

    CMV IE

    Available Since

    Sept. 9, 2014

    Availability

    Academic Institutions and Nonprofits only

40. pCMVtrunc F-tractinN9-39-msfGFPΔN7

    Plasmid

    #231552

    Purpose

    Mammalian expression of C-terminally truncated F-tractin fused to N-terminally truncated monomeric sfGFP, for actin filament organization measurements using fluorescence polarization microscopy

    Depositor

    Manos Mavrakis

    Article

    Martins et al Cell. 2025 Apr 1:S0092-8674(25)00276-4.

    Insert

    F-tractin (Itpka Rat)
    

    Tags

    msfGFP

    Expression

    Mammalian

    Promoter

    CMVtrunc

    Available Since

    Jan. 30, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge