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Plasmid#137785PurposeVisualization of the Beta-2-adrenergic receptorDepositorInsertHuman beta2-adrenergic receptor (ADRB2 Human)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-L1460P
Plasmid#69006Purposeactivating MTOR mutationDepositorInsertMTOR-L1460P (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Leucine 1460 to ProlinePromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-S2215Y
Plasmid#69013Purposeactivating MTOR mutationDepositorInsertMTOR (MTOR Human)
UseTagsFLAGExpressionMammalianMutationS2215YPromoterCMVAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β2B Tubulin
Plasmid#170553PurposeEncodes codon-optimized human α1B and β2B tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-I2500F
Plasmid#69014Purposeactivating MTOR mutationDepositorInsertMTOR-I2500F (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Isoleucine 2500 to PhenylalaninePromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-C1483F
Plasmid#69008Purposeactivating MTOR mutationDepositorInsertMTOR-C1483F (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Cysteine 1483 to PhenylalaninePromoterAvailable sinceJan. 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-V2006I
Plasmid#69012Purposeactivating MTOR mutationDepositorInsertMTOR (MTOR Human)
UseTagsFLAGExpressionMammalianMutationV2006IPromoterCMVAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-E1799K
Plasmid#69009Purposeactivating MTOR mutationDepositorInsertMTOR-E1799K (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Glutamate 1799 to LysinePromoterAvailable sinceDec. 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCMV-Myc CDC4 WT*
Plasmid#16652DepositorInsertCDC4 (FBXW7 Human)
UseTagsmycExpressionMammalianMutation*Note that this insert is only WT relative to the…PromoterAvailable sinceMarch 12, 2008AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-R2505P
Plasmid#69015Purposeactivating MTOR mutationDepositorInsertMTOR-R2505P (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Arginine 2505 to ProlinePromoterAvailable sinceDec. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
sAB-K29
Plasmid#204735Purposesynthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitinDepositorInsertsynthetic antigen-binding fragment that can specifically recognize K29-linked polyubiquitin
UseTagsExpressionMutationPromoterAvailable sinceOct. 27, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMXS-IRES-BLAST TFR1
Plasmid#169890PurposeExpress TFR1 open reading frame. Lacks IRE regulatory elementsDepositorInsertTFRC (TFRC Human)
UseRetroviralTagsExpressionMammalianMutationTFRC cDNA, lacking 3'UTR regulatory elementsPromoterAvailable sinceMay 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFastBac Dual α1B/β3 Tubulin
Plasmid#170552PurposeEncodes codon-optimized human α1B and β3 tubulin for expression in and purification from insect cells.DepositorUseTagsAP linker, GS linker (GGSGG), His10 tag, L21 enha…ExpressionInsectMutationPromoterAvailable sinceJune 15, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-T1977R
Plasmid#69011Purposeactivating MTOR mutationDepositorInsertMTOR-T1977R (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Threonine 1977 to ArgininePromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pPEPX-P3-sgRNAluc
Plasmid#85590PurposeIntegrate plasmid of Streptococcus pneumoniae, which can integrate sgRNA targeting luc gene, which encode luciferase, into the locus between amiF and treR. This vector can be used as the template forDepositorInsertsgRNA targeting firefly luciferase encoding gene
UseCRISPRTagsExpressionBacterialMutationPromoterP3Available sinceJan. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-MTOR-F1888L
Plasmid#69010Purposeactivating MTOR mutationDepositorInsertMTOR-F1888L (MTOR Human)
UseTagsFLAGExpressionMammalianMutationchange Phenylalanine 1888 to LeucinePromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
CAGGS-EGFP-NLS-3XFLAG-V5
Plasmid#196090PurposeExpresses gfp in mammalian cells. Contains a c-terminal 3xFlag tag and a c-terminal V5 tagDepositorInsertGFP
UseTagsC-terminal 3xFLAG and C-terminal V5ExpressionBacterial and MammalianMutationPromoterCAGGsAvailable sinceSept. 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTH727-CEN-RLuc/staCFLuc
Plasmid#38211DepositorInsertsFirefly Luciferase
Renilla luciferase
UseTagsExpressionYeastMutationThe last three codons of the full-length Firefly …PromoterADH1 and TDH3 (=GPD)Available sinceOct. 22, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro-HA-PIK3CA(H1047R;F486S;del)
Plasmid#33255DepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHemagglutinin (HA)ExpressionMammalianMutationchanged histidine 1047 to arginine and phenylalan…PromoterSV40Available sinceJan. 4, 2012AvailabilityAcademic Institutions and Nonprofits only -
LIC-Z
Plasmid#153543Purposeperform light induced optogenetic clustering of TCR ζ-Chain as described in the associated publicationDepositorInserthuman TCR ζ-Chain (CD247 Human)
UseTagsmCherryExpressionMammalianMutationNAPromoterCMVAvailable sinceJuly 17, 2020AvailabilityAcademic Institutions and Nonprofits only