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pGW1-ExSYTE-EGFP-DTE

Plasmid

#216260

Purpose

ExSYTE - EGFP fusion with 3'UTR of Rat Arc DTE

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

ExSYTE EGFP - Rat DTE

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-DR

Plasmid

#216266

Purpose

Dox-responsive module fused with EGFP

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

Codon-optimized TetR - Lipid binding motif (KRf - KLK) - Codon-optimized TetR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pEGFP-C2-KRf-TetR

Plasmid

#216269

Purpose

Construct 2: Lipid binding domain KRφ - TetR

Depositor

Susumu Tomita

Article

Park et al Cell Rep. 2023 Aug 29;42(8):112826. doi:

Insert

Lipid binding motif (KRf) - TetR

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

May 8, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV.cc81-mut

Plasmid

#199745

Purpose

Expresses AAV2 Rep and AAV.cc81 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc81 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV.cc84-mut

Plasmid

#199746

Purpose

Expresses AAV2 Rep and AAV.cc84 Cap

Depositor

Aravind Asokan

Article

Gonzalez et al Nat Protoc. 2023 Sep 21. doi: 10.1038/s4

Insert

AAV.cc84 Cap

Use

AAV

Tags

Expression

Mammalian

Mutation

mutated amino acids 586-592 in Cap

Promoter

Available since

Oct. 31, 2023

Availability

Academic Institutions and Nonprofits only

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p8291 MSCV-Neo C-HA 18E7 ΔDLLC

Plasmid

#184523

Purpose

Expression of HPV18 E7 ΔDLLC

Depositor

Elizabeth White

Article

Hatterschide et al Elife. 2022 Feb 16;11. pii: 75466. doi:

Insert

HPV18 E7 ΔDLLC

Use

Retroviral

Tags

HA

Expression

Mutation

deletion of DLLC

Promoter

MSCV LTR

Available since

June 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCDF-pntAB

Plasmid

#158609

Purpose

Low phosphate induction of pntAB

Depositor

Michael Lynch

Article

Li et al Metab Eng. 2021 Jan 15. pii: S1096-7176(

Insert

pntAB(membrane bound proton translocating pyridine nucleotide transhydrogenase)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Insulated ugpBp from E. coli

Available since

Jan. 31, 2022

Availability

Academic Institutions and Nonprofits only

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S111

Plasmid

#174549

Purpose

Constitutive PhCMV-driven mammalian expression vector of CDH-1-TF cassette (PhCMV-Gal4-Bcl-XL-P2A-LD3-Rel65-pA)

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

BclxL and designed protein LD3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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S112

Plasmid

#174550

Purpose

Constitutive PhCMV-driven mammalian expression vector of CDH-2-TF cassette (PhCMV-Gal4-Bcl2-P2A-LD3-Rel65-pA)

Depositor

Bruno Correia

Article

Shui et al Nat Commun. 2021 Oct 1;12(1):5754. doi:

Insert

Bcl2 and designed protein LD3

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Spa)_gcrA3

Plasmid

#133343

Purpose

for constitutive expression of a single guide RNA from Streptococcus pasteurianus with a seed region that targets gcrA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_gcrA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pBXMCS-2 Pconstitutive-sgRNA(Sth3)_blaA

Plasmid

#133345

Purpose

for constitutive expression of a single guide RNA from Streptococcus thermophilus #3 with a seed region that targets blaA gene's promoter from Caulobacter crescentus

Depositor

Michael Laub

Article

Guzzo et al mBio. 2020 Jan 14;11(1):e02415-19. doi:

Insert

sgRNA_blaA

Use

CRISPR

Tags

Expression

Mutation

Promoter

constitutive

Available since

Jan. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pPB-CAG-zNanogHD-pgk-hph

Plasmid

#74910

Purpose

Mammalian expression of zNanog Homeodomain

Depositor

Jose Silva

Article

Theunissen et al Development. 2011 Nov;138(22):4853-65. d

Insert

Nanog

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG

Available since

May 17, 2016

Availability

Academic Institutions and Nonprofits only

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pNH (GB0064)

Plasmid

#68165

Purpose

Provides the S. Lycopersicum fruit promoter NH as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlNH promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pPUbc (GB0145)

Plasmid

#68170

Purpose

Provides the S. Lycopersicum Ubiquitin carrier protein promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlUbc promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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p2x35S (GB0222)

Plasmid

#68173

Purpose

Provides the CaMV dual 35S Promoter as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

2x35S promoter

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only
p6xUAS (GB0179)

Plasmid

#68182

Purpose

Provides 6xUAS (upstream activating sequence) as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

6xUAS

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

March 16, 2016

Availability

Academic Institutions and Nonprofits only

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pDGB3_omega1R

Plasmid

#68240

Purpose

GoldenBraid level 1 Omega plant expression vector

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

empty vector

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 29, 2015

Availability

Academic Institutions and Nonprofits only

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pT2A11 (GB0143)

Plasmid

#68189

Purpose

Provides the S. Lycopersicum 2A11 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

Sl2A11 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 23, 2015

Availability

Academic Institutions and Nonprofits only

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pTE8 (GB0144)

Plasmid

#68190

Purpose

Provides the S. Lycopersicum E8 terminator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

SlE8 terminator

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pLexAOp (GB0118)

Plasmid

#68181

Purpose

Provides the 4xLexA Operator as a level 0 GoldenBraid part

Depositor

Diego Orzaez

Article

Sarrion-Perdigones et al Plant Physiol. 2013 Jul;162(3):1618-31.

Insert

4xOpLexA

Use

Synthetic Biology

Tags

Expression

Mutation

BsaI and BsmBI sites removed

Promoter

Available since

Oct. 20, 2015

Availability

Academic Institutions and Nonprofits only

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