We narrowed to 12,360 results for: NUC

1. pKJ234

   Plasmid

   #217492

   Purpose

   Mammlian expression of DpFnuc for genome editing

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   DpFnuc
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKJ217

   Plasmid

   #212318

   Purpose

   Express MmFnuc with pureexpress

   Depositor

   Jonathan Gootenberg, Omar Abudayyeh

   Article

   Jiang et al Sci Adv. 2023 Sep 29;9(39):eadk0171. doi

   Insert

   Mercenaria
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Jan. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. KBB382

   Plasmid

   #185085

   Purpose

   HA-tagged POM152 with glycosylation sites mutated

   Depositor

   Ken Belanger

   Article

   Belanger et al Genetics. 2005 Nov;171(3):935-47. doi: 1

   Insert

   POM152
   

   Use

   Tags

   HA

   Expression

   Yeast

   Mutation

   Pom152 N280A, N398A, N???A, N???A = Pom152Dglyc

   Promoter

   Available since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

4. pDZ584 pKAN 1x-mCherry

   Plasmid

   #73172

   Purpose

   Floxed Kanamycin (G418) selection maker; 3' tagging vector used to create C-terminal fusion proteins with 1x-mCherry

   Depositor

   Daniel Zenklusen

   Article

   Saroufim et al J Cell Biol. 2015 Dec 21;211(6):1131-40.

   Insert

   1x-mCherry
   

   Use

   Unspecified

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Aug. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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5. pJK040 (pET-PurQ-A128T)

   Plasmid

   #73840

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, A128T mutant (PurQ-A128T)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   A128T mutant

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pJK044 (pET-PurQ-wt)

   Plasmid

   #73839

   Purpose

   Produces Bacillus subtilis formylglycinamidine ribonucleotide synthetase glutaminase subunit, (PurQ)

   Depositor

   JoAnne Stubbe, T. J. Kappock

   Article

   Hoskins et al Biochemistry. 2004 Aug 17;43(32):10314-2

   Insert

   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 11, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pJK598

   Plasmid

   #72523

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with C-terminal His6 tag, and associated hypothetical protein (AaPurS, AaOrfY, AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   6xHis

   Expression

   Bacterial

   Mutation

   synthetic gene

   Promoter

   T7 and n/a

   Available since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pJK612

   Plasmid

   #72533

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurL)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pJK613

   Plasmid

   #72534

   Purpose

   Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase subunits, one with His6 tag; associated hypothetical protein N-terminal domain (AaPurS, AaOrfY[1-69], AaPurQ, AaPurLH6)

   Depositor

   T. J. Kappock

   Inserts

   formylglycinamidine ribonucleotide synthetase, small subunit
   hypothetical protein in purSQL operon
   formylglycinamidine ribonucleotide synthetase, glutaminase subunit
   formylglycinamidine ribonucleotide synthetase, large subunit
   

   Use

   Tags

   His6

   Expression

   Bacterial

   Mutation

   residues 1-69 only; C-terminal domain removed and…

   Promoter

   T7 and n/a

   Available since

   Jan. 29, 2016

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pJK619

    Plasmid

    #72536

    Purpose

    Produces Acetobacter aceti 1023 formylglycinamidine ribonucleotide synthetase small subunit, N-term His-tag; hypothet. protein; glutaminase subunit; large subunit (H6AaPurS, AaOrfY, AaPurQ, AaPurL)

    Depositor

    T. J. Kappock

    Inserts

    formylglycinamidine ribonucleotide synthetase, small subunit
    hypothetical protein in purSQL operon
    formylglycinamidine ribonucleotide synthetase, glutaminase subunit
    formylglycinamidine ribonucleotide synthetase, large subunit
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    synthetic gene

    Promoter

    T7 and n/a

    Available since

    Jan. 29, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. esgRNA_NbPDS

    Plasmid

    #231147

    Purpose

    T-DNA encoding TRV2 with mobile gRNA targeting NbPDS

    Depositor

    Daniel Voytas

    Article

    Liu et al Plant Physiol. 2024 Mar 29;194(4):2229-2

    Insert

    mobile gRNA targeting NbPDS
    

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    PEBV sub-genomic

    Available since

    March 6, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCX-2A-2A

    Plasmid

    #74670

    Purpose

    based on pCX-C1 with F2A1/F2A2 inserted

    Depositor

    Roberto Giovannoni

    Article

    De Giorgi et al Plasmid. 2015 May;79:22-9. doi: 10.1016/

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    pCAGGS

    Available since

    Feb. 14, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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13. FokI-dCas9

    Plasmid

    #52970

    Purpose

    Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

    Depositor

    David Liu

    Article

    Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

    Insert

    Fok1 fused to dCas9
    

    Use

    CRISPR

    Tags

    Fok1 nuclease (homodimeric wildtype) and NLS

    Expression

    Mammalian

    Mutation

    D10A and H840A in Cas9

    Promoter

    CMV

    Available since

    May 21, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. NLS-SuperNova

    Plasmid

    #131858

    Purpose

    Expresses SuperNova fused to nuclear localization signal (NLS)

    Depositor

    Geert van den Bogaart

    Article

    Paardekooper et al Int J Mol Sci. 2019 Aug 25;20(17). pii:

    Insert

    SuperNova
    

    Use

    Tags

    Myc tag and c-myc nuclear localization signal (NL…

    Expression

    Mammalian

    Mutation

    Promoter

    cmv

    Available since

    Oct. 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pX330-LMNA-gRNA1

    Plasmid

    #122507

    Purpose

    expresses WT spCas9 and a chimeric gRNA targeting human LMNA

    Depositor

    Graham Dellaire

    Article

    Pinder et al Nucleic Acids Res. 2015 Oct 30;43(19):93

    Insert

    LMNA-gRNA1
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    April 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCS2-Tol1.zf1

    Plasmid

    #61388

    Purpose

    mRNA synthesis of tol1 transposase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    tol1.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCS2-Cre.zf1

    Plasmid

    #61391

    Purpose

    mRNA synthesis of Cre recombinase codon modified for zebrafish

    Depositor

    Harold Burgess

    Article

    Horstick et al Nucleic Acids Res. 2015 Jan 27. pii: gkv

    Insert

    Cre.zf1
    

    Use

    Zebrafish expression

    Tags

    Expression

    Mutation

    Promoter

    sp6

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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18. Human PNP his-tagged

    Plasmid

    #64076

    Purpose

    Expression of human Purine Nucleoside Phosphorylase

    Depositor

    Vern Schramm

    Article

    Lewandowicz et al J Biol Chem. 2003 Aug 22;278(34):31465-8

    Insert

    Purine Nucleoside Phosphorylase (PNP Human)
    

    Use

    Tags

    Histidine tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    April 29, 2015

    Availability

    Academic Institutions and Nonprofits only

19. pEGFP-C1-Nup35

    Plasmid

    #87342

    Purpose

    To express human Nup35 in mammalian cells

    Depositor

    Lei Lu

    Article

    Tie et al Biochem Biophys Res Commun. 2016 Sep 30;

    Insert

    nucleoporin 35 (NUP35 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pSDM052

    Plasmid

    #194341

    Purpose

    pUC mini-Tn7 vector expressing LseCas13a

    Depositor

    Joseph Bondy-Denomy

    Article

    Mendoza et al Nature. 2020 Jan;577(7789):244-248. doi:

    Insert

    Cas13a(Lse)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge