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pTagGFP2-N SIN1 delta69-97

Plasmid

#124924

Purpose

For expression of a.a.69-97 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-RAD52-WT

Plasmid

#235676

Purpose

Expresses HA-tagged wild type human RAD52 in mammalian cells.

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Insert

RAD52 (RAD52 Human)

Tags

HA

Expression

Mammalian

Mutation

wild type

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.3 ss-3xFLAG-hLRP5 (aa 32-1615)

Plasmid

#115788

Purpose

Expresses human LRP5 with N-terminal 3xflag tag in mammalian cells, contains artificial signal sequence upstream of tag.

Depositor

Harald Junge

Article

Lai et al Cell Rep. 2017 Jun 27;19(13):2809-2822.

Insert

LRP5 (LRP5 Human)

Tags

3xflag

Expression

Mammalian

Mutation

contains silent mutation that creates an internal…

Promoter

CMV

Available Since

Oct. 22, 2018

Availability

Academic Institutions and Nonprofits only

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pLM-AMA18.0 dCas9-VPR

Plasmid

#138945

Purpose

Fungal AMA1 backbone plasmid with sp-dCas9 fused to VP64-p65-Rta (VPR); ribozymes based sgRNA transcription unit; Terbinafine and Phleomycin selection markers

Depositor

Arnold Driessen

Article

Mozsik et al Sci Rep. 2021 Jan 13;11(1):1118. doi: 10

Insert

p40S:sp_dCas9m4_2xNLS-VPR; HH-HDV sgRNA transcription unit, Terbinafine (ergA) and Phleomycin (ble) selection markers

Use

CRISPR and Synthetic Biology; Ama1 autonomously r…

Tags

NLS

Promoter

p40S (AN0465) Aspergillus nidulans

Available Since

Jan. 19, 2021

Availability

Academic Institutions and Nonprofits only

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pEGFP-ARAP3

Plasmid

#39484

Depositor

Sonja Vermeren

Article

Krugmann et al Mol Cell. 2002 Jan;9(1):95-108.

Insert

ARAP3 (ARAP3 Human)

Tags

EGFP

Expression

Mammalian

Available Since

Aug. 22, 2012

Availability

Academic Institutions and Nonprofits only
LPUtopia-7

Plasmid

#199212

Purpose

Landing Pad Utopia_v7 donor plasmid for AAVS1 site-specific integration in human cells. Use NeoR and eGFP as positive selection marker for AAVS1-targeted insertion and RFP as negative selection marker

Depositor

Gabor Balazsi

Article

Wan et al Nat Chem Biol. 2023 Jul;19(7):887-899. d

Inserts

aminoglycoside phosphotransferase,
enhanced Green Fluorescence Protein
Red Fluorescence Protein

Expression

Mammalian

Promoter

CMV and thymidine kinase promoter

Available Since

Aug. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 deltaCRIM

Plasmid

#124921

Purpose

For expression of Conserved Region In Middle deletion mutant of SIN1-GFP (delta139-267)

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 deltaPH

Plasmid

#124922

Purpose

For expression of Pleckstrin Homology domain deletion mutant of SIN1-GFP (delta376-486)

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3-EFNB2-RBD-Fc (D62Q-Q130L-V167L)

Plasmid

#200980

Purpose

Mammalian expression plasmid for soluble EFNB2 (D62Q-Q130L-V167L specificity mutant) fused to IgG1 Fc

Depositor

Erik Procko

Article

Narayanan et al J Virol. 2023 Nov 30;97(11):e0062123. do

Insert

EFNB2 (EFNB2 Human)

Tags

IgG1 Fc

Expression

Mammalian

Mutation

Soluble Receptor-Binding Domain; D62Q-Q130L-V167L…

Promoter

CMV

Available Since

Oct. 2, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3e- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192239

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3e

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3e-knockin-homology arm-mAID-mClover-NeoR (EIF3E Human)

Expression

Mammalian

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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Donor_YTHDF2_KO_Neo

Plasmid

#186672

Purpose

Donor plasmid to endogenously knock out the ANKRD1 in Hek293 cells. Neomycin and polyA sequence is inserted between two homology arms.

Depositor

Astrid Haase

Article

Meng et al Nucleic Acids Res. 2022 May 27. pii: 659

Insert

YTHDF2 KO Neomycin (YTHDF2 Human)

Expression

Mammalian

Available Since

Aug. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta95-114

Plasmid

#124925

Purpose

For expression of a.a.95-114 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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pTagGFP2-N SIN1 delta116-127

Plasmid

#124926

Purpose

For expression of a.a.116-127 deletion mutant of SIN1-GFP

Depositor

Jin Chen

Article

Hwang et al Cancer Res. 2019 Jul 1;79(13):3178-3184.

Insert

MAPKAP1 (MAPKAP1 Human)

Tags

TagGFP2

Expression

Mammalian

Mutation

Insert ORF was sirently mutated to be resistent t…

Available Since

May 20, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.GFP

Plasmid

#59404

Purpose

expresses eGFP from a 400 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

400 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

pFOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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Lenti.pFOXA2.5'.GFP

Plasmid

#59407

Purpose

expresses eGFP from an about 1350 bp human FOXA2 promoter to select floor plate and ventral midbrain progenitor cells during development

Depositor

Kai-Christian Sonntag

Article

Aguila et al Stem Cells Transl Med. 2014 Jul 14. pii:

Insert

approx. 1350 bp FOXA2 promoter, eGFP (FOXA2 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

FOXA2

Available Since

April 19, 2016

Availability

Academic Institutions and Nonprofits only

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pEHC_PGKneoLox2DTA.2

Plasmid

#112623

Purpose

H2BEmerald_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-Emerald (H2BC3 Human, Aequorea victoria)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

Emerald

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV-pBG-mCRE98-NLS-EGFP-WPRE

Plasmid

#239977

Purpose

adeno-associated virus (AAV) encoding enhanced green fluorescent protein (eGFP) under motor neuron-selective control of minimal beta globin promoter (pBG) and CRE98

Depositor

Sinisa Hrvatin

Article

Nagy et al Proc Natl Acad Sci U S A. 2024 Dec 3;121

Insert

EGFP (eGFP Synthetic)

Use

AAV

Promoter

pBG + mCRE98

Available Since

June 26, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-HA-RAD52-∆C

Plasmid

#235677

Purpose

Expresses HA-tagged mutant human RAD52 in mammalian cells with a C-terminal deletion (Δ302-410 amino acids).

Depositor

Simon Powell

Article

Jalan et al Nat Commun. 2024 Sep 5;15(1):7776. doi:

Insert

RAD52 (RAD52 Human)

Tags

HA

Expression

Mammalian

Mutation

deleted amino acids 302-410

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pPL001-BBTK

Plasmid

#184751

Purpose

Big-IN positive control payload encoding EF1a-driven GFP-T2A-BSD. Harbors a backbone counterselectable TK cassette.

Depositor

Jef Boeke

Article

Brosh et al Proc Natl Acad Sci U S A. 2021 Mar 9;118

Insert

pEF1a-eGFP-T2A-BSD-bGHpA

Use

Cre/Lox, Mouse Targeting, and Synthetic Biology ;…

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 17, 2023

Availability

Academic Institutions and Nonprofits only

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ptdTHC_PGKneoLox2DTA.2

Plasmid

#112625

Purpose

H2BtdTomato_p2A_HygroR_p2A_CreERt2 targeting plasmid with cloning sites ready for homology arms

Depositor

Stefan Heller

Article

Hartman et al Dev Biol. 2018 Sep 1. pii: S0012-1606(18

Inserts

H2B-tdTomato (H2BC3 Anthozoa, Human)
Hygrophosphotransferase
Cre-ERT2 fusion protein

Use

Cre/Lox and Mouse Targeting

Tags

tdTomato

Promoter

No Promoter

Available Since

Sept. 13, 2018

Availability

Academic Institutions and Nonprofits only

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