We narrowed to 3,458 results for: cgas

Showing: 641 - 660 of 3458 results

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SL2-sgSat-MTSb/BFP/pdCas9-C1

Plasmid

#162761

Purpose

Expressing dCas9 and sgRNA containg MTSa targeting centromeres

Depositor

Antony K Chen

Article

Wu et al Nucleic Acids Res. 2018 Jul 27;46(13):e8

Insert

dCas9 and sgRNA(SL2-sgSat-MTSb)

Expression

Mammalian

Mutation

dCas9(nuclease deactivated Cas9)

Promoter

U6/CMV

Available Since

March 23, 2021

Availability

Academic Institutions and Nonprofits only

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pCC20

Plasmid

#198783

Purpose

Drives specific expression in the ASK neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Psra-9-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJMP1102

Plasmid

#119246

Purpose

knockdown phzA1 in P. aeruginosa

Depositor

Carol Gross, Jason Peters, Oren Rosenberg

Article

Peters et al Nat Microbiol. 2019 Jan 7. pii: 10.1038/

Insert

sgRNA phzA1 (P. aeruginosa)

Expression

Bacterial

Mutation

D10A and H840A

Available Since

Feb. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-Teton-puro-shFAK #1

Plasmid

#198757

Purpose

conditional knockdown of FAK

Depositor

Hyonchol Jang

Article

Kim et al Cancers (Basel). 2019 Sep 2;11(9). pii:

Insert

shFAK #1 (PTK2 Human)

Expression

Mammalian

Available Since

April 21, 2023

Availability

Academic Institutions and Nonprofits only

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PGL3-U6-ALDOB_pegRNA-Csy4RS-nick_sgRNA-EGFP

Plasmid

#172668

Purpose

For expression of transcript containing pegRNA and nick-sgRNA targeting human ALDOB gene from the U6 promoter with pegRNA flanked by Csy4 recognition site

Depositor

Xiaolong Wang

Article

Liu et al Cell Res. 2021 Jun 8. pii: 10.1038/s4142

Insert

ALDOB pegRNA and ALDOB_nick-sgRNA

Expression

Mammalian

Promoter

U6

Available Since

Nov. 15, 2021

Availability

Academic Institutions and Nonprofits only

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1161F

Plasmid

#183138

Purpose

Plasmid supports expression of 2 gRNAs targeting D.suzukii sxl, and 3 gRNA targeting D.suzukii bTub,Hr5Ie1-eGFP tagged, and can be integrated with pBac.

Depositor

Omar Akbari

Article

Kandul et al GEN Biotechnology (2022) 1:4, 372-385

Insert

U6.3-gRNAs[sxl, bTub]

Use

CRISPR

Expression

Insect

Available Since

Oct. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shPRDM14-2

Plasmid

#193695

Purpose

Constitutive lentiviral expression of PRDM14 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

PRDM14 (PRDM14 Human)

Use

Lentiviral

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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pT7-SpCas9_sgRNA-site2 (RTW448)

Plasmid

#160137

Purpose

T7 promoter expression plasmid for in vitro transcription of SpCas9 sgRNA with spacer #2

Depositor

Keith Joung, Benjamin Kleinstiver

Article

Walton et al Nat Protoc. 2021 Feb 5. pii: 10.1038/s41

Insert

SpCas9 sgRNA with spacer #2 (spacer=GTCGCCCTCGAACTTCACCT)

Use

In vitro transcription of sgrna from t7 promoter

Promoter

T7

Available Since

Feb. 8, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX2TK-YAP-WW1-RRK

Plasmid

#104306

Purpose

bacterial expression of RKK mutant WW domain 1 of YAP; RRK mutant binds to PPxY where Y is phosphorylated

Depositor

Marius Sudol

Insert

YAP WW1 domain (YAP1 Human)

Tags

GST

Expression

Bacterial

Mutation

WW domain 1 RRK mutant (TTAAATCACATCGATCAG to AGA…

Promoter

tac

Available Since

Jan. 2, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.U6gLacZ.hSyn.H2B.RFP

Plasmid

#170369

Purpose

Negative control plasmid used for Crispr/cas9 based disruption. Expressing nuclear RFP.

Depositor

Johan Jakobsson

Article

Jonsson et al EMBO J. 2021 Mar 1:e106423. doi: 10.1525

Insert

H2B RFP

Use

AAV

Promoter

human Synapsin

Available Since

Jan. 4, 2022

Availability

Academic Institutions and Nonprofits only

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GG-EBNA-TdT-guide1-PGK-Puro

Plasmid

#102903

Purpose

EBNA episome plasmid for U6 promoter-driven expression of a control gRNA targeting TdTomato sequence. Includes PGK-puro selection cassette.

Depositor

Timo Otonkoski

Article

Weltner et al Nat Commun. 2018 Jul 6;9(1):2643. doi: 1

Insert

TdT-guide1-PGK-Puro

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

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pKDsgRNA-hisG

Plasmid

#89954

Purpose

Contains arabinose-induced lambda Red and a tet-inducible gRNA targeting hisG.

Depositor

Michael Lynch

Article

Moreb et al ACS Synth Biol. 2017 Oct 2. doi: 10.1021

Insert

hisG gRNA

Expression

Bacterial

Promoter

pTet

Available Since

July 12, 2017

Availability

Academic Institutions and Nonprofits only

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pTC441

Plasmid

#91218

Purpose

protoplast vector for targeted deletion of MLO gene in barley (PvUbi1 promoter driving the gRNA array)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting MLO gene in barley

Use

CRISPR

Expression

Plant

Available Since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pTC437

Plasmid

#91219

Purpose

protoplast vector for targeted deletion of MLO gene in barley (CmYLCV promoter driving the gRNA array)

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Insert

gRNAs targeting MLO gene in barley

Use

CRISPR

Expression

Plant

Available Since

July 11, 2017

Availability

Academic Institutions and Nonprofits only

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pQdCas12a.sggfp(A)

Plasmid

#236038

Purpose

The plasmid pQdCas12a.sggfp(A) expresses the dCas12a endonuclease and the sgRNA (design A) targeting the gfp gene.

Depositor

Radhakrishnan Mahadevan

Article

Golla et al ACS Synth Biol. 2025 Oct 17;14(10):3890-

Insert

quorum sensing cassette luxI, luxR, and the LuxR-dependent promoter pLuxI , dCas12a and sgRNA design (A) of gfp gene

Use

CRISPR

Promoter

quorum sensing promoter

Available Since

April 24, 2025

Availability

Academic Institutions and Nonprofits only

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pLX304 mNG PH PLCD1

Plasmid

#233254

Purpose

Expression of mNeonGreen-tagged PH domain of PLCD1

Depositor

David Bryant

Article

Nikolatou et al EMBO J. 2023 Sep 18;42(18):e113987. doi:

Insert

PLCD1

Use

Lentiviral

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCF821-recipient_U6-sgRNA-EF1a-mNeonGreen

Plasmid

#211651

Purpose

U6-sgRNA-EF1a-mNeonGreen

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCF820-recipient_U6-sgRNA-EF1a-mCherry2

Plasmid

#211647

Purpose

U6-sgRNA-EF1a-mCherry2

Depositor

Christof Fellmann

Article

Tan et al Cell Rep. 2023 Nov 28;42(11):113339. doi

Insert

Guide RNA recipient vector

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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pWP052

Plasmid

#198774

Purpose

Drives specific expression in the ALN, PLN neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pnlp-20-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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pWP003

Plasmid

#198803

Purpose

Drives specific expression in the URX neurons

Depositor

Paul Sternberg

Article

Nava et al Proc Natl Acad Sci U S A. 2023 Dec 19;12

Insert

Pflp-8-GAL4-SK(DBD)-VP64

Expression

Worm

Available Since

May 3, 2023

Availability

Academic Institutions and Nonprofits only

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