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pL0_AB-OEE3

Plasmid

#202103

Purpose

Level 0 plasmid containing the promoter from gene 54499 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

OEE3 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-P49

Plasmid

#202102

Purpose

Level 0 plasmid containing the promoter from gene 49202 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

P49 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-NR

Plasmid

#202101

Purpose

Level 0 plasmid containing the promoter from gene 54983 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

NR promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pL0_AB-H4

Plasmid

#202100

Purpose

Level 0 plasmid containing the promoter from gene 34971 from Phaeodactylum tricornutum with AB overhangs used to build a level 1 construct.

Depositor

Christopher Dupont

Article

Pollak et al Synth Biol (Oxf). 2020;5(1):ysaa001. doi

Insert

H4 promoter

Use

Synthetic Biology

Mutation

None

Available Since

Aug. 23, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA4.TO_ORF18_TurboID_3xHA

Plasmid

#200017

Purpose

Expresses KSHV protein ORF18 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF18

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF30_TurboID_3xHA

Plasmid

#200018

Purpose

Expresses KSHV protein ORF30 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF30

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF31_TurboID_3xHA

Plasmid

#200019

Purpose

Expresses KSHV protein ORF31 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF31

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_ORF66_TurboID_3xHA

Plasmid

#200020

Purpose

Expresses KSHV protein ORF66 fused at the C-terminal to TurboID-3xHA

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF66

Tags

TurboID-3xHA

Expression

Mammalian

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCDNA4.TO_3xHA_TurboID_ORF34

Plasmid

#200021

Purpose

Expresses KSHV protein ORF34 fused at the N-terminal to 3xHA-TurboID

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF34

Tags

3xHA-TurboID

Expression

Mammalian

Promoter

CMV promoter

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-zeo G226A ORF29

Plasmid

#200024

Purpose

Lentiviral vector for expression of KSHV ORF29 with G226A mutation

Depositor

Britt Glaunsinger

Article

McCollum et al PLoS Pathog. 2023 Apr 17;19(4):e1011163.

Insert

ORF29

Use

Lentiviral

Mutation

Glycine 226 changed to alanine

Promoter

CMV

Available Since

July 20, 2023

Availability

Academic Institutions and Nonprofits only

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pET-CVm-Sec22b

Plasmid

#168029

Purpose

Expresses eCFP-Sec22b(A131-H190)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-Sec22b(A131-H190)-mVenus

Tags

Hisx6 and myc

Expression

Bacterial

Available Since

April 20, 2021

Availability

Academic Institutions and Nonprofits only

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pET-CVm-VAMP8

Plasmid

#168028

Purpose

Expresses eCFP-VAMP8(G7-R67)-mVenus in bacterial cells

Depositor

David Liu

Article

Blum et al Science. 2021 Feb 19;371(6531):803-810.

Insert

eCFP-VAMP8(G7-R67)-mVenus

Tags

Hisx6 and myc

Expression

Bacterial

Available Since

April 6, 2021

Availability

Academic Institutions and Nonprofits only

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Antares-SEPLuc IRESv24

Plasmid

#141087

Purpose

precursor of the bioluminescent reporter pHluc, for detecting extracellular pH changes in tumor acidosis; utilizes weaker mutant of IRES

Depositor

Jeak Ling Ding

Article

Ong et al Front Bioeng Biotechnol. 2020 May 8;8:41

Insert

Antares-T2A-puromycin-IRESv24-SEPLuc

Expression

Mammalian

Promoter

CMV

Available Since

May 11, 2020

Availability

Academic Institutions and Nonprofits only

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Ta1098 E140A

Plasmid

#19085

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (TA_RS05685 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E140A

Available Since

Dec. 31, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D21A

Plasmid

#19073

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E18A/D21A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 D21A

Plasmid

#19074

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

D21A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 D22A

Plasmid

#19075

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

D22A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 D34A

Plasmid

#19076

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

D34A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A

Plasmid

#19077

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E18A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
Ta1098 E18A/D22A

Plasmid

#19078

Depositor

SeWon Suh

Article

Lee et al Mol Cell. 2007 Sep 21. 27(6):938-50.

Insert

Cell division protein pelota related protein (DOM34 Thermoplasma acidophilum)

Tags

C tag

Expression

Bacterial

Mutation

E18A/D22A

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only