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Plasmid#215969PurposeFragmid fragment: (Pol 2 promoter) Gal4-induced expressionDepositorHas ServiceCloning Grade DNAInsertLoxP_v1; 5xUAS_v1; LoxP_v1; 5xUAS_v1; Hsp70_v1
UseFragmentTagsExpressionMutationPromoterAvailable sinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pT2h70CRET(#325)
Plasmid#184060Purposecyclofen-inducible CRE activation in zebrafish transient transgenicDepositorInsertCRE-ERT2
UseZebrafish transient transgenesisTagsExpressionMutationERT2: G400V, M543A, L544A, deleted 1-281;PromoterZf-Hsp70-4Available sinceJuly 11, 2022AvailabilityAcademic Institutions and Nonprofits only -
D32_Ld_354780
Plasmid#135848PurposeExpression of Leishmania donovani ectodomain in mammalian cellsDepositorInsertHsp70 protein, putative
UseTagsbiotinylation sequence and His tagExpressionMammalianMutationPromoterAvailable sinceJuly 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJFRC81-3xVAS1-Syn21-Citrine-HA-P10
Plasmid#104616PurposeExpresses HA tagged Citrine upon binding of TALE1 to VAS1DepositorInsertSyn21-Citrine-HA-P10-MhcSD
UseTagsHAExpressionInsectMutationPromoterhsp70Available sinceJan. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
p3.2mar(T->G)-GFP
Plasmid#63695Purpose3.2 kb marine armor plate enhancer with a single T->G bp change driving GFP reporterDepositorInsert3.2 kb marine stickleback armor plate enhancer with a single T->G base pair change
UseTagsEGFPExpressionMammalianMutationT->G bp changePromoterhsp70Available sinceApril 1, 2015AvailabilityAcademic Institutions and Nonprofits only -
hs-FL-Kek1-myc2
Plasmid#41170DepositorInsertKek1 (kek1 Fly)
UseTags2X mycExpressionInsectMutationPromoterHsp70Available sinceJan. 25, 2013AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-10H-SIII
Plasmid#175444PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal 10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-10His-TwinStrep and BiP Secretion PeptideExpressionInsectMutationPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-GFP
Plasmid#175363PurposeZeoR overexpression of cytosolic GFP in Drosophila ExpreS2ion cellsDepositorInsertsgGFP
UseTagsExpressionInsectMutationF64L, S65C, and I167TPromoterFused Actin-HSP70Available sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
FLAG-MED17
Plasmid#15410DepositorInsertMED17 (MED17 Human)
UseTagsFLAGExpressionMammalianMutationN/APromoterAvailable sinceAug. 15, 2007AvailabilityAcademic Institutions and Nonprofits only -
pExpreS2-1-C3-PA-10H-SIII
Plasmid#175443PurposeHiFi-compatible destination vector for secreted overexpression of 3C-cleavable, C-terminal Protein A-10His-TwinStrep tagged proteins with ExpreS2 PlatformDepositorTypeEmpty backboneUseTags3C-Protein A-10His-TwinStrep and BiP Secretion Pe…ExpressionInsectMutationPromoterFused Actin-HSP70Available sinceApril 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6s
Plasmid#62812PurposepPBC-G6s expresses PiggyBac inverted terminal repeat-flanked GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6s
Plasmid#62809PurposepPBC-LG6s expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6s under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6s-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G6f
Plasmid#62811PurposepPBC-G6f expresses PiggyBac inverted terminal repeat-flanked GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP6f-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 22, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-LG6f
Plasmid#62808PurposepPBC-LG6f expresses PiggyBac inverted terminal repeat-flanked Lck-GCaMP6f under the control of the CAG promoter.DepositorInsertITR-CAG-Lck-GCaMP6f-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 19, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPBC-G3-tdT
Plasmid#62814PurposepPBC-G3-tdT expresses PiggyBac inverted terminal repeat-flanked GCaMP3 and tdTomato under the control of the CAG promoter.DepositorInsertITR-CAG-GCaMP3-IRES-tdTomato-ITR
UseTagsExpressionBacterial and MammalianMutationPromoterCAGAvailable sinceMay 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pLdCH
Plasmid#84291PurposeExpress gRNA and Cas9 in Leishmania with Hygromycin resistanceDepositorInsertgRNA and Cas9
UseCRISPR; LeishmaniaTagsExpressionMutationPromoterL. donovani ribosome RNA promoterAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
HS-EB3-GFP
Plasmid#105948Purposeheat shock inducible transgenesisDepositorInsertEB3 (MAPRE3 Human)
UseTagsEGFPExpressionBacterialMutationPromoterAvailable sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196E
Plasmid#52975Purposeinsect expression of SIK3 T196E constitutively active mutantDepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196E constitutively active mutantPromoterHsp70Available sinceMay 20, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 T196A
Plasmid#52974Purposeinsect expression of SIK3 T196A (LKB1 phosphorylation defective mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationT196A LKB1 phosphorylation defective mutantPromoterHsp70Available sinceMay 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pUAST-SIK3 K70M
Plasmid#52973Purposeinsect expression of SIK3 K70M (kinase dead mutant)DepositorInsertSIK3 (Sik3 Fly)
UseTagsHAExpressionInsectMutationK70M kinase dead mutantPromoterHsp70Available sinceMay 5, 2014AvailabilityAcademic Institutions and Nonprofits only