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pLEW100V5-BSD

Plasmid

#27658

Depositor

George Cross

Insert

BSD

Use

Trypanosme expression

Available since

Aug. 19, 2011

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pSL3617 (pCRISPR(BbsI)_Pse)

Plasmid

#200902

Purpose

Expresses Pseudoalteromonas CRISPR RNA from a U6 promoter. The CRISPR array contains two BbsI sites for spacer cloning.

Depositor

Samuel H. Sternberg

Publication

Lampe et al Nat Biotechnol. 2023 Mar 29. doi: 10.103

Insert

PseCRISPR (entry)

Expression

Mammalian

Available since

May 22, 2023

Availability

Academic Institutions and Nonprofits only

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pLenti spCas9 T2A iRFP670 P2A puro

Plasmid

#122182

Purpose

The plasmid codes for a Flag-spCas9 protein, a iRFP670 fluorescent protein and a puromycin resistance. The plasmid has two BsmBI acceptor sites to insert gRNA expressing sequences.

Depositor

Raphael Gaudin

Inserts

spCas9 (NEWENTRY )
iRFP670

Use

Lentiviral

Tags

T2A

Expression

Mammalian

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pLew100_myc_BirA*

Plasmid

#41716

Depositor

Graham Warren

Publication

Morriswood et al Eukaryot Cell. 2012 Dec 21.

Type

Empty backbone

Use

T. brucei expression

Promoter

PARP

Available since

March 26, 2013

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles
pHR-H13LTat-CD8a/d2eGFP-IRES-Nef

Plasmid

#126552

Purpose

Replication incompetent HIV with H13L Tat and d2eGFP/CD8a reporter

Depositor

Jonathan Karn

Publication

Dobrowolski et al MBio. 2019 Mar 26;10(2). pii: mBio.00337

Insert

HIV-1 vector pNL4-3

Use

Lentiviral

Tags

CD8a and GFP

Mutation

Delta gag/pol

Promoter

HIV LTR

Available since

July 2, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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p3E-mTagBFP-CAAX

Plasmid

#75176

Purpose

Membrane targeted TagBFP (blue) fluorophore with stop codon for C-terminal fusions

Depositor

Nicholas Cole

Publication

Don et al Zebrafish. 2016 Sep 15.

Insert

mTagBFP-CAAX

Use

Three prime entry vector for multisite gateway th…

Promoter

n/a

Available since

Oct. 6, 2016

Availability

Academic Institutions and Nonprofits only

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pRK172-6xHis-0N4R-P301S-tau-HiBiT

Plasmid

#187835

Purpose

Expression of 6xHis-tagged P301S tau of 0N4R isoform with a C-terminal HiBiT tag for bimolecular luminescence with LgBiT.

Depositor

William McEwan

Publication

Tuck et al Cell Rep. 2022 May 3;39(5):110776. doi:

Insert

6XHis-0N4R-P301S-tau-HiBiT

Tags

6xHistidine and HiBiT

Expression

Bacterial

Promoter

T7

Available since

Sept. 22, 2023

Availability

Academic Institutions and Nonprofits only

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MXS_CMV::PuroR-bGHpA

Plasmid

#62439

Purpose

MXS_chaining vector with CMV::PuroR-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

resistance cassette against Puromycin with CMV Promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pENTR L3-4XeGFP-Myc-L2

Plasmid

#32316

Depositor

Steve Stowers

Publication

Petersen et al PLoS One. 2011;6(9):e24531. Epub 2011 Se

Insert

4XeGFP-Myc

Use

Gateway multisite entry vector

Tags

Myc

Available since

Sept. 15, 2011

Availability

Academic Institutions and Nonprofits only

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Fus-BcLOV-mCh-SOS

Plasmid

#208275

Purpose

Expresses Fus-BcLOV-SOS for optogenetic activation of Ras/Erk signaling. Includes an mCherry tag.

Depositor

Lukasz Bugaj

Publication

Pal et al Proc Natl Acad Sci U S A. 2023 Aug 8;120

Insert

Fus-BcLOV-mCh-SOS

Use

Lentiviral

Promoter

SFFV

Available since

Jan. 25, 2024

Availability

Academic Institutions and Nonprofits only

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MXS_PGK::rtTA3-bGHpA

Plasmid

#62446

Purpose

MXS_chaining vector with PGK::rtTA3-bGHpA

Depositor

Pierre Neveu

Publication

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Insert

cassette containing the reverse tetracycline transactivator with PGK promoter

Use

Synthetic Biology

Available since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

Cited by

2 Articles

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pQTEV-YWHAE

Plasmid

#31562

Depositor

Konrad Buessow

Publication

Bussow et al Microb Cell Fact. 2005 Jul 5;4:21. doi:

Insert

tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, epsilon (YWHAE Human)

Tags

His and TEV

Expression

Bacterial

Available since

March 22, 2013

Availability

Academic Institutions and Nonprofits only

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pJBL7010

Plasmid

#136942

Purpose

Constitutive sfGFP expression (high), bacterial promoter

Depositor

Julius Lucks

Publication

Silverman et al ACS Synth Biol. 2019 Feb 15;8(2):403-414

Insert

sfGFP

Use

Synthetic Biology

Expression

Bacterial

Promoter

J23119

Available since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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B-GRAM-W

Plasmid

#211707

Purpose

Expression of a dimerization-dependent fluorescent protein (ddFP) subunit (B) fused with the GRAM domain of GRAMD1b carrying a G187W mutation (B-GRAM-W) in mammalian cells

Depositor

Yasunori Saheki

Publication

Koh et al Nat Commun. 2023 Oct 25;14(1):6773. doi:

Insert

B-tagged GRAM domain of GRAMD1b/Aster-B (GRAMD1B Human)

Tags

ddFP-B3

Expression

Mammalian

Mutation

Changed Glycine 187 to Tryptophan

Promoter

CMV

Available since

Jan. 16, 2024

Availability

Academic Institutions and Nonprofits only

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pSB1K3 containing mRFP1

Plasmid

#118082

Purpose

For course in "Synthetic Biology: A Lab Manual"

Depositor

Anthony Forster

Publication

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, mRFP1

Use

Escherichia coli

Available since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pUC19-U6-LwaCas13acrRNA-BsmBIcassette (LTH151)

Plasmid

#171129

Purpose

LwaCas13a crRNA entry vector for pU6 expression (need to clone in spacer into BsmBI sites)

Depositor

Benjamin Kleinstiver

Publication

Johnson et al Mol Cell. 2022 Jun 2;82(11):2161-2166.e3

Insert

U6 promoter entry plasmid for LwaCas13a crRNA cloning

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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RRL.sin.cPPT.SFFV/Flag-Firefly luciferase.IRES-puro.WPRE (MT05)

Plasmid

#139446

Purpose

Lentiviral vector genome to ectopically express Firefly luciferase

Depositor

Caroline Goujon

Publication

Doyle et al Nat Microbiol. 2018 Dec;3(12):1369-1376.

Insert

FLAG-tag Firefly luciferase

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

SFFV

Available since

Aug. 12, 2020

Availability

Academic Institutions and Nonprofits only

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pJN105-Hygro-GFP

Plasmid

#41883

Depositor

Howard Steinman

Publication

Bandyopadhyay et al Infect Immun. 2004 Aug;72(8):4541-51.

Insert

Ptac::GFP-mut-2

Use

Bacterial broad host range vector

Expression

Bacterial

Mutation

GFP-mut-2 is GFP with Ser65Ala, Val68Leu, and Ser…

Promoter

Ptac

Available since

June 11, 2013

Availability

Academic Institutions and Nonprofits only
pT7sgRNA

Plasmid

#111820

Purpose

Expresses sgRNA in T. brucei cells

Depositor

David Horn

Publication

Rico et al Sci Rep. 2018 May 21;8(1):7960. doi: 10.

Insert

sgRNA

Use

CRISPR

Available since

July 10, 2018

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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pYPQ262-ABE8e

Plasmid

#161523

Purpose

Gateway entry clone (attL1 & attR5) for CRISPR-zSpCas9 ABE8e mediated A-G base editing

Depositor

Yiping Qi

Publication

Ren et al Nat Plants. 2021 Jan 4. pii: 10.1038/s41

Insert

ABE8e-zSpCas9(D10A)

Use

CRISPR; Gateway compatible abe8e-zspcas9(d10a) en…

Expression

Plant

Available since

Feb. 26, 2021

Availability

Academic Institutions and Nonprofits only

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