We narrowed to 7,632 results for: ALP

Showing: 6581 - 6600 of 7632 results

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pTag4C_Nrg1-GV-2HA

Plasmid

#227104

Purpose

Nrg1 protein for luciferase and barcode assays

Depositor

Michael Wehr

Article

Popovic et al Biomed Pharmacother. 2024 Nov;180:117523

Insert

Nrg1-GV (Nrg1 Mouse)

Tags

2xHA

Expression

Mammalian

Available Since

Dec. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pAG517

Plasmid

#226735

Purpose

Expresses a reporter for an alpha-helical OMM protein

Depositor

Jonathan Weissman

Article

Guna et al Science. 2022 Oct 21;378(6617):317-322.

Insert

BCL2L1 (BCL2L1 Human)

Use

Lentiviral

Tags

GFP11

Available Since

Oct. 24, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-rTetR(SE)-HP1a-2A-mTurquoise

Plasmid

#225691

Purpose

Lentiviral expression of rTetR(SE) fused to HP1a and mTurquoise-NLS

Depositor

Lacra Bintu

Article

Sinha et al bioRxiv [Preprint]. 2023 Oct 14:2023.10.

Insert

HP1a (CBX5 Synthetic, Human)

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG and T2A-mTurquoise

Expression

Mammalian

Available Since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pMBS-838

Plasmid

#217994

Purpose

pLEXSY_I-blecherry3_dom_lacZ Destination Vector

Depositor

Michael Schroda

Article

Hieronimus et al Microb Cell. 2024 Apr 30;11:128-142. doi

Type

Empty backbone

Use

Synthetic Biology

Promoter

T7 promotor

Available Since

Aug. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pHIV-NAT-T2A-hCD52-eGFP-NFATC2IP-SPR

Plasmid

#221447

Purpose

Lentiviral mammalian expression vector of eGFP-tagged NFATC2IP ORF that is fused with S-phase restricted (SPR) degron at its C-terminus

Depositor

Daniel Durocher

Article

Cho et al Genes Dev. 2024 Apr 17;38(5-6):233-252.

Insert

NFATC2IP (NFATC2IP Human)

Use

Lentiviral

Tags

eGFP

Expression

Mammalian

Mutation

SPR degron: hGEM (1-110) fragment that is fused t…

Promoter

CMV

Available Since

July 3, 2024

Availability

Industry, Academic Institutions, and Nonprofits

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pAAV-G12-hFGF1

Plasmid

#219554

Purpose

hFGF1 expression in mammalian cells. pAAV vector. Reporter plasmid.

Depositor

Vladislav Verkhusha

Article

Baloban et al Protein Sci. 2024 May;33(5):e4993. doi:

Insert

hFGF1 (FGF1 Human)

Use

AAV

Expression

Mammalian

Promoter

12xUAS with TATA-box minimal promoter

Available Since

May 23, 2024

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI + DomII pcDNA3

Plasmid

#206094

Purpose

expression of the N-terminus, Domain I, Domain II and the II-III loop (amino acids 1-1154) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Bn CAAX pcDNA3

Plasmid

#206088

Purpose

Cav2.2 N-terminus (amino acids 1-95) with a C-terminal tag of the final 10 aa of H.Ras containing CAAX motif

Depositor

Annette Dolphin

Article

Page et al J Biol Chem. 2010 Jan 8;285(2):835-44. d

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 20, 2023

Availability

Academic Institutions and Nonprofits only

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qTAG-N-Solo-sTagRFP-TUBA1B

Plasmid

#207765

Purpose

Donor template for sTagRFP insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Insert

TUBA1B Homology Arms flanking a sTagRFP Tag (TUBA1B Human)

Use

CRISPR; Donor template

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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Cav2.2 pCAGGS

Plasmid

#206101

Purpose

expression of rabbit Cav2.2 calcium channel, the 3'UTR is deleted, this enhances the expression

Depositor

Annette Dolphin

Article

Page et al J Neurosci. 2004 Jun 9;24(23):5400-9. do

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

CMV/B-actin

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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GFP Cav2.2 W391A pRK5

Plasmid

#206084

Purpose

expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2 that prevents Beta subunit binding

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Tags

GFP

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Oct. 25, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 DomI pMT2

Plasmid

#206127

Purpose

expression of the N-terminus, Domain I and the I-II loop (amino acids 1-483) of rabbit Cav2.2 calcium channel

Depositor

Annette Dolphin

Article

Raghib et al J Neurosci. 2001 Nov 1;21(21):8495-504.

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Promoter

Ad MLP/TPL/SV40

Available Since

Oct. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pGL4.23-Gja1prom

Plasmid

#188114

Purpose

Expresses luciferase under control of Gja1 promoter in transfected cells

Depositor

Jennifer Mitchell

Article

Shchuka et al PLoS One. 2023 Jan 3;18(1):e0271081. doi

Insert

Gja1 promoter (Gja1 Mouse)

Use

Luciferase

Promoter

Gja1 endogenous promoter up to gene start codon

Available Since

Sept. 28, 2023

Availability

Academic Institutions and Nonprofits only

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Cav2.2 HA W391A pcDNA3

Plasmid

#206071

Purpose

expression of rabbit Cav2.2 calcium channel with an exofacial double HA tag in domain II and a mutation (W391A) in the ?1-interacting domain (AID) in the I-II loop of Cav2.2

Depositor

Annette Dolphin

Article

Waithe et al J Biol Chem. 2011 Mar 18;286(11):9598-61

Insert

cacna1b (CACNA1B Rabbit)

Expression

Mammalian

Mutation

W391A

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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Halo-S13S2DBD

Plasmid

#200196

Purpose

Expression of Sox13 with Sox2 DBD

Depositor

Sheila Teves

Article

Price et al Nucleic Acids Res. 2023 Apr 28:gkad304.

Insert

Halo-S13S2DBD (Sox2, Sox13 Mouse)

Tags

Halo

Expression

Mammalian

Mutation

Sox13(1-1242); Sox 2 (126-333); Sox13 (1449-1839)

Promoter

EF_1alpha

Available Since

Aug. 28, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA 3.1 - circMAN2A1

Plasmid

#198837

Purpose

This contains the 400nt isoform of exons two and three from MAN2A1 circular RNA, 3X Flag tag inserted in exon two

Depositor

Stefan Stamm

Article

Arizaca Maquera et al Front Mol Neurosci. 2023 May 5;16:114107

Insert

circular mannosidase alpha class 2A member 1

Expression

Mammalian

Mutation

circular RNA composed by 2 exons

Promoter

CMV

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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mCherry-FKBP-PIP4K2AD273K

Plasmid

#202733

Purpose

expresses recruitable fluorescent protein-tagged enzyme

Depositor

Gerry Hammond

Article

Wills et al J Cell Sci. 2023 Aug 3:jcs.261494. doi:

Insert

mCherry:FKBP1A(3-108):[GGSA]4GG:PIP4K2A (D266K) (PIP4K2A, FKBP1A Human)

Expression

Mammalian

Available Since

June 28, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet_Seq1.2

Plasmid

#201404

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/shCol1a1/Scarlet-Seq1.1

Plasmid

#201401

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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pT2/Col1a1/GFP4_Seq 1.1

Plasmid

#201400

Purpose

Knockdown of Collagen Type 1 alpha 1. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

Depositor

Maria Castro

Article

Comba et al Nat Commun. 2022 Jun 24;13(1):3606. doi:

Insert

COL1A1

Expression

Mammalian

Available Since

May 24, 2023

Availability

Academic Institutions and Nonprofits only

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