19,658 results

1. C1(1-29)-miniTurbo-V5_pLX304

   Plasmid

   #107176

   Purpose

   expresses V5-tagged miniTurbo on the mammalian ER membrane, lentiviral vector

   Depositor

   Alice Ting

   Article

   Branon et al Nat Biotechnol. 2018 Aug 20. pii: nbt.42

   Insert

   miniTurbo (BirA mutant)
   

   Use

   Lentiviral

   Mutation

   aa1-63 deleted; Q65P, I87V, R118S, E140K, Q141R, …

   Available Since

   May 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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2. mNeonGreen-JIP4

   Plasmid

   #164621

   Purpose

   Expresses JIP4 in mammalian cells with a mNeonGreen tag

   Depositor

   Mark Cookson

   Article

   Bonet-Ponce et al Sci Adv. 2020 Nov 11;6(46). pii: 6/46/ea

   Insert

   JIP4 (SPAG9 Human)
   

   Tags

   mNeonGreen

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 3, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCMV6M-Pak1

   Plasmid

   #12209

   Depositor

   Jonathan Chernoff

   Article

   Sells et al Curr Biol. 1997 Mar 1. 7(3):202-10.

   Insert

   Pak1 (PAK1 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Aug. 11, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. PB-TO-MYOD1-shOct4

   Plasmid

   #182309

   Purpose

   Piggybac Tet-ON plasmid for differentiating hiPSCs into skeletal muscle cells via MYOD1 and shOCT4 expression

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Inserts

   MYOD1-shOct4 (MYOD1 Human)
   puro-T2A-mycNLS-mTagBFP2
   

   Tags

   T2A-mycNLS-mTagBFP2

   Expression

   Mammalian

   Mutation

   shOct4

   Promoter

   EF1a and TRE3G

   Available Since

   April 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. AAV pCAG-FLEX-tdTomato-WPRE

   Plasmid

   #51503

   Purpose

   Can be used to generate AAV virus that will express tdTomato in the presence of Cre

   Depositor

   Hongkui Zeng

   Article

   Oh et al Nature. 2014 Apr 10;508(7495):207-14. do

   Insert

   tdTomato
   

   Use

   AAV

   Promoter

   pCAG

   Available Since

   Aug. 29, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTriExRhoA2G

   Plasmid

   #40176

   Depositor

   Olivier Pertz

   Article

   Fritz et al Sci Signal. 2013 Jul 23;6(285):rs12. doi

   Insert

   RhoA Biosensor
   

   Tags

   His-Myc

   Expression

   Bacterial, Insect, and Mamm…

   Promoter

   CMV

   Available Since

   July 5, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. pKLV2-EF1a-Cas9Bsd-W

   Plasmid

   #68343

   Purpose

   Lentiviral vector expressing Cas9 fused with the Blasticidin resistant gene at the C-terminus

   Depositor

   Kosuke Yusa

   Article

   Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

   Insert

   EF1a-Cas9 cassette, WPRE
   

   Use

   CRISPR and Lentiviral

   Tags

   Cas9 fused with Flag-tag and a NLS at the N termi…

   Promoter

   EF1a

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. Pflp-18::NGL-1::spGFP11

   Plasmid

   #65828

   Purpose

   For trans-synaptic labeling

   Depositor

   Cori Bargmann, Kang Shen

   Article

   Feinberg et al Neuron. 2008 Feb 7. 57(3):353-63.

   Insert

   NLG-1 (nlg-1 Nematode)
   

   Tags

   spGFP11

   Expression

   Worm

   Mutation

   N142S

   Promoter

   Pflp-18

   Available Since

   Nov. 20, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEGFP-Kif2A

   Plasmid

   #52401

   Purpose

   Expresses GFP-tagged Kif2A in mammalian cells under the CMV promoter

   Depositor

   Gohta Goshima, Ryota Uehara

   Article

   Uehara et al J Cell Biol. 2013 Aug 19;202(4):623-36.

   Insert

   Kif2A (KIF2A Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. pAAVS1-TRE-MA4-GFP

    Plasmid

    #114380

    Purpose

    Doxycycline inducible MLL-AF4 and GFP expression. The construct has been used with CAG-rtTA for making engraftable iPSC derived HSCs.

    Depositor

    Yuet Wai Kan

    Article

    Tan et al Proc Natl Acad Sci U S A. 2018 Feb 27;11

    Insert

    MLL-AF4 fusion gene (AFF1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    TRE

    Available Since

    Feb. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. PKC zeta DN

    Plasmid

    #21249

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC zeta (Prkcz Rat)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K281M

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

12. pScarlet-N1

    Plasmid

    #128060

    Purpose

    Mammalian expression vector for C terminal mScarlet-I fusions

    Depositor

    Oskar Laur

    Type

    Empty backbone

    Tags

    mScarlet-I

    Expression

    Mammalian

    Available Since

    Aug. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pET28a_T7-ARG1

    Plasmid

    #106473

    Purpose

    Expresses acoustic reporter genes or gas vesicles (ARG1) in Escherichia coli

    Depositor

    Mikhail Shapiro

    Article

    Bourdeau et al Nature. 2018 Jan 3;553(7686):86-90. doi:

    Insert

    ARG1
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 23, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pLSSmKate2-N1

    Plasmid

    #31867

    Depositor

    Vladislav Verkhusha

    Article

    Piatkevich et al Proc Natl Acad Sci U S A. 2010 Mar 23;10

    Insert

    LSS-mKate2
    

    Expression

    Mammalian

    Mutation

    K69Y/P131T/S148G/M167D/T183S/M196V

    Promoter

    CMV

    Available Since

    Sept. 13, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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15. mCherry-Rab11a-7

    Plasmid

    #55124

    Purpose

    Localization: Ras GTPase, Excitation: 587, Emission: 610

    Depositor

    Michael Davidson

    Insert

    Rab11a (RAB11A Human)
    

    Tags

    mCherry

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 29, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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16. SpyTag-β-Lactamase-SpyCatcher

    Plasmid

    #52656

    Purpose

    Expresses SpyTag-SpyCatcher mediated cyclized version of TEM-1 β-lactamase (BLA). Cyclization of BLA led to an increase in thermal resilience of >60 degrees celsius.

    Depositor

    Mark Howarth

    Article

    Schoene et al Angew Chem Int Ed Engl. 2014 Jun 10;53(2

    Insert

    TEM-1 β-Lactamase
    

    Tags

    SpyCatcher and SpyTag

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    May 1, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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17. 458 p5E-uasE1b

    Plasmid

    #26026

    Depositor

    Nathan Lawson

    Insert

    E1B basal promoter + UAS repeats
    

    Use

    Entry vector

    Available Since

    Aug. 26, 2010

    Availability

    Academic Institutions and Nonprofits only

    

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18. pGP-CMV-jGCaMP7s

    Plasmid

    #104463

    Purpose

    Mammalian expression of ultrasensitive protein calcium sensor

    Depositor

    Douglas Kim, GENIE Project

    Article

    Dana et al Nat Methods. 2019 Jul;16(7):649-657. doi

    Insert

    jGCaMP7s
    

    Tags

    T7 epitope, Xpress tag, 6xHis

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCDH-EF1-Luc2-P2A-copGFP

    Plasmid

    #72485

    Purpose

    Express Luc2 and copGFP

    Depositor

    Kazuhiro Oka

    Insert

    Luc2-P2A-copGFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF-1

    Available Since

    Jan. 11, 2016

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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20. Flag-HA-USP36

    Plasmid

    #22579

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    USP36 (USP36 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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