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pPOTv6-blast-His:mNG:His

Plasmid

#201094

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

mNeonGreen

Use

Unspecified

Promoter

read through

Available Since

July 29, 2024

Availability

Academic Institutions and Nonprofits only

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pDonorCm(Ptr)

Plasmid

#175577

Purpose

DNA donor for transposition

Depositor

Sheng Yang

Article

Yang et al Nucleic Acids Res. 2021 Sep 3. pii: 6363

Insert

Mini-Tn, Cm cassette

Expression

Bacterial

Mutation

NO

Available Since

Nov. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pZE31ms2

Plasmid

#25860

Depositor

Philippe Cluzel

Article

Le et al Proc Natl Acad Sci U S A. 2005 Jun 28. 1

Insert

ms2 binding site (x2)

Expression

Bacterial

Mutation

none

Available Since

Nov. 2, 2010

Availability

Academic Institutions and Nonprofits only

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VKG1-gRNA-pX330

Plasmid

#108671

Purpose

Cleavage of VKG1 sequence by CRISPR/Cas9

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

gRNA for VKG1 sequence

Use

CRISPR

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pGZ136 (pRS406-pREB1-3FLAG-MNase-NLS)

Plasmid

#72273

Purpose

FLAG-Mnase expressed under the REB1 promoter

Depositor

Steven Henikoff

Article

Zentner et al Nat Commun. 2015 Oct 22;6:8733. doi: 10.

Insert

3xFLAG tag and aa 83-231 of Mnase

Promoter

REB1

Available Since

Jan. 6, 2016

Availability

Academic Institutions and Nonprofits only
pVKG1-Puro

Plasmid

#108670

Purpose

Puromycin resistant cassette for knock-in by VIKING method

Depositor

Shigeo Sugano

Article

Sawatsubashi et al Sci Rep. 2018 Jan 12;8(1):593. doi: 10.1

Insert

PURO

Use

CRISPR and Synthetic Biology

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-3FLAG

Plasmid

#221045

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

3xFLAG epitopes

Use

Unspecified

Promoter

read through

Available Since

Aug. 5, 2024

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-mKate2 no-pA

Plasmid

#80828

Purpose

P2A followed by mKate2 without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-mKate2 no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pME-FlEx switch

Plasmid

#82406

Purpose

Gateway-compatible FlEx cassette containing two pairs of heterotypic, antiparallel loxP-type recombination sites

Depositor

Judith Eisen

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Type

Empty backbone

Available Since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pCSIIbsr-GCaMP6s

Plasmid

#184712

Purpose

Calcium biosensor

Depositor

Michiyuki Matsuda

Article

Watabe et al eLife. 2024 Jan 26;12:RP86727. doi: 10.7

Insert

GCaMP6s

Use

Lentiviral

Expression

Mammalian

Available Since

Jan. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoTagRFP-T-SpHis5

Plasmid

#44842

Purpose

Yeast tagging vector to create gene fusion with yeast optimized TagRFP-T with SpHis5 selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoTagRFP-T

Expression

Yeast

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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pPOTv6-puro-3Ty:tagRFPt:3Ty

Plasmid

#201089

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

July 12, 2023

Availability

Academic Institutions and Nonprofits only

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pFCK(1.3)GW

Plasmid

#27230

Depositor

Pavel Osten

Article

Dittgen et al Proc Natl Acad Sci U S A. 2004 Dec 28. 1

Insert

EGFP

Use

Lentiviral

Tags

EGFP

Available Since

March 11, 2011

Availability

Academic Institutions and Nonprofits only

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pPOTv7-blast-tagRFPt

Plasmid

#179790

Purpose

Trypanosoma brucei PCR only tagging (pPOT) vector for endogenous gene tagging

Depositor

Samuel Dean, Keith Gull

Article

Paterou et al Open Biol. 2025 Apr;15(4):240361. doi: 1

Insert

tagRFPt

Use

Unspecified

Promoter

read through

Available Since

April 24, 2023

Availability

Academic Institutions and Nonprofits only

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p3E-nlsGFP no-pA

Plasmid

#80815

Purpose

nlsGFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-CMV-nlsGFP no pA

Expression

Mammalian

Available Since

Nov. 1, 2016

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp7

Plasmid

#163591

Purpose

Expresses BMP-7 in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp7 (Bmp7 Mouse)

Expression

Mammalian

Available Since

Aug. 24, 2021

Availability

Academic Institutions and Nonprofits only

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pFA6a-link-yoLSS-mKate2-Kan

Plasmid

#44943

Purpose

Yeast tagging vector to create gene fusion with yeast optimized LSS-mKate2 with Kan selection

Depositor

Wendell Lim, Kurt Thorn

Article

Lee et al PLoS One. 2013 Jul 2;8(7):e67902. doi: 1

Insert

yoLSS-mKate2

Expression

Yeast

Available Since

Oct. 18, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-Bmp8a

Plasmid

#163592

Purpose

Expresses BMP-8A in mammalian cells

Depositor

Takeshi Imai

Article

Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

Insert

Bmp8a (Bmp8a Mouse)

Expression

Mammalian

Available Since

Aug. 23, 2021

Availability

Academic Institutions and Nonprofits only

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pLB2 Ubi-FLIP FF

Plasmid

#19753

Depositor

Richard Hynes

Article

Stern et al Proc Natl Acad Sci U S A. 2008 Sep 16. 1

Insert

oligo targeting Firefly luciferase

Use

Cre/Lox, Lentiviral, Mouse Targeting, and RNAi

Expression

Mammalian

Available Since

Dec. 18, 2008

Availability

Academic Institutions and Nonprofits only

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pLenti-EF1a-SOX2

Plasmid

#35388

Depositor

Feng Zhang

Article

Zhang et al Nat Biotechnol. 2011 Feb;29(2):149-53. d

Insert

TALE-Sox2

Use

Lentiviral

Expression

Mammalian

Available Since

March 29, 2012

Availability

Academic Institutions and Nonprofits only