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pScalps_Puro_mTet2 catalytic domain HxD

Plasmid

#79611

Purpose

Expression of catalytically inactive mouse Tet2

Depositor

Silvia Monticelli

Article

Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

Insert

Tet2 (Tet2 Mouse)

Use

Lentiviral

Tags

Myc

Mutation

Mutant Tet2 with H1302Y, D1304A substitutions in …

Available Since

July 21, 2016

Availability

Academic Institutions and Nonprofits only
pJCSUP35(1-253)

Plasmid

#1089

Depositor

Susan Lindquist

Article

Glover et al Cell 1997 May 30;89(5):811-9.

Insert

SUP35 (SUP35 Budding Yeast)

Tags

His

Expression

Bacterial

Mutation

amino acids 1 - 253 of SUP35

Available Since

July 18, 2005

Availability

Academic Institutions and Nonprofits only
4xMS2-hTR

Plasmid

#154940

Purpose

4xMS2 stem-loop-hTR-500 bp genomic sequence

Depositor

Alice Ting

Article

Han et al Proc Natl Acad Sci U S A. 2020 Aug 24. p

Insert

4xMS2 stem-loop-hTR-500 bp genomic sequence

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 4, 2020

Availability

Academic Institutions and Nonprofits only

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pENTR-p38KTRClover

Plasmid

#59142

Purpose

ORF encoding p38 KTR mClover flanked by Gateway sequences

Depositor

Markus Covert

Article

Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

Insert

p38 Kinase Translocation Reporter (MAPK14 Mouse, Human)

Available Since

Sept. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FBL6-FLAG3C

Plasmid

#19904

Depositor

Yue Xiong

Article

Hu et al Genes Dev. 2008 Apr 1. 22(7):866-71.

Insert

FBL6 (FBXL6 Human)

Tags

FLAG3

Expression

Mammalian

Available Since

April 1, 2009

Availability

Academic Institutions and Nonprofits only

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p3E-P2A-GFP no-pA

Plasmid

#80826

Purpose

P2A followed by GFP without pA

Depositor

Philip Washbourne

Article

Fowler et al PLoS One. 2016 Aug 8;11(8):e0159277. doi

Insert

p3E-P2A-GFP no-pA

Expression

Mammalian

Available Since

Oct. 20, 2016

Availability

Academic Institutions and Nonprofits only

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pENTR-NICD1

Plasmid

#46048

Depositor

Xin Chen

Article

Fan et al J Clin Invest. 2012 Aug 1;122(8):2911-5.

Insert

Notch1 (C-term) (Notch1 Mouse)

Use

Gateway Cloning

Tags

Myc tag

Mutation

Cytoplasmic domain initiating at amino acid 1753

Available Since

July 2, 2013

Availability

Academic Institutions and Nonprofits only
pK037.CAG-FRT-stop-FRT-RFP-ires-tTA-WPRE (Supernova)

Plasmid

#85038

Purpose

For Flpe/FRT-based Supernova-RFP, pK037 should be used with pK036.

Depositor

Takuji Iwasato

Article

Luo et al Sci Rep. 2016 Oct 24;6:35747. doi: 10.10

Insert

FRT-stop-FRT

Expression

Mammalian

Available Since

Nov. 30, 2016

Availability

Academic Institutions and Nonprofits only

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AAV-Syn-PSD95-mCherry

Plasmid

#125694

Purpose

Exogenous expression of PSD95 (red fluorescence)

Depositor

Xue Han

Article

Bensussen et al iScience. 2020 Jun 30;23(7):101330. doi:

Insert

PSD95-mCherry

Use

AAV

Expression

Mammalian

Promoter

Synapsin

Available Since

July 2, 2020

Availability

Academic Institutions and Nonprofits only

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Fibronectin-human-EDA in pMAX

Plasmid

#120402

Purpose

enables eukaryotic expression of human EDA (or EIIIA) containing fibronectin based on plasma fibronectin sequence

Depositor

Inaam Nakchbandi

Article

Rossnagl et al PLoS Biol. 2016 Sep 21;14(9):e1002562. d

Insert

EDA-fibronectin (FN1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

July 23, 2019

Availability

Academic Institutions and Nonprofits only

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pGH00.0126

Plasmid

#64257

Purpose

Binary T-DNA vector expressing EGFP and neomycin phosphotransferase II (NPTII); for Agrobacterium tumefaciens-mediated transformation

Depositor

Mark Guiltinan, Siela Maximova

Article

Maximova et al Plant Cell Rep. 2003 Jun;21(9):872-83. E

Inserts

EGFP
neomycin phosphotransferase II

Use

Binary agrobacterium vector for plant transformat…

Expression

Plant

Promoter

E12-omega

Available Since

June 10, 2015

Availability

Academic Institutions and Nonprofits only

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PB533-44B12m23-sfGFP

Plasmid

#186778

Purpose

RNAP2 Ser5ph-mintbody expression vector for mammalian cells

Depositor

Hiroshi Kimura

Article

Ohishi et al Nat Commun. 2022 Dec 20;13(1):7672. doi:

Insert

44B12m23-scFv

Tags

sfGFP

Expression

Mammalian

Available Since

Aug. 9, 2022

Availability

Academic Institutions and Nonprofits only

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scOrange chromoprotein

Plasmid

#117840

Purpose

BioBrick pSB1C3 plasmid that constitutively overexpresses scOrange chromoprotein in E. coli

Depositor

Anthony Forster

Article

Liljeruhm et al J Biol Eng. 2018 May 10;12:8. doi: 10.11

Insert

promoter, RBS, scOrange

Use

Synthetic Biology; Escherichia coli

Mutation

BioBrick sites removed

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-hs-KRAS

Plasmid

#52729

Purpose

retroviral expression of human KRAS

Depositor

Shinya Yamanaka

Article

Worringer et al Cell Stem Cell. 2014 Jan 2;14(1):40-52.

Insert

KRAS (KRAS Human)

Use

Retroviral

Expression

Mammalian

Available Since

April 8, 2015

Availability

Academic Institutions and Nonprofits only

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pCMV-PE7-P2A-BSD

Plasmid

#224122

Purpose

Mammalian expression of SpCas9 PE7 prime editor with P2A-BSD marker

Depositor

Brittany Adamson

Article

Yan et al Nature. 2024 Apr 3. doi: 10.1038/s41586-

Insert

PE7-P2A-BSD

Tags

P2A-BSD, SV40 bpNLS, and c-Myc NLS

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Plasmid

#129631

Purpose

Visualization of Akt and ERK activity

Depositor

Dorus Gadella

Article

Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

Insert

H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

Expression

Mammalian

Promoter

CMV

Available Since

July 28, 2021

Availability

Academic Institutions and Nonprofits only

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pRU294

Plasmid

#167689

Purpose

Final destination vector for single Gateway LR reaction

Depositor

Niko Geldner

Article

Ursache et al Plant Methods. 2021 Oct 30;17(1):111. do

Insert

pEC1.2::Cas9i

Use

CRISPR

Available Since

July 20, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV-Sst44-GFP

Plasmid

#172310

Purpose

SST interneuron-restricted gene regulatory element GRE44, to drive GFP expression in SST+ interneurons

Depositor

Michael Greenberg

Article

Hrvatin et al Elife. 2019 Sep 23;8. pii: 48089. doi: 1

Insert

Sst44

Use

AAV

Promoter

pB-Globin

Available Since

July 14, 2021

Availability

Academic Institutions and Nonprofits only

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pQE30-HyPer7

Plasmid

#141071

Purpose

Bacterial expression of ultrasensitive hydrogen peroxide indicator HyPer7 for optical imaging

Depositor

Vsevolod Belousov

Article

Pak et al Cell Metab. 2020 Mar 3;31(3):642-653.e6.

Insert

HyPer7

Tags

Hisx6

Expression

Bacterial

Promoter

T5

Available Since

March 1, 2021

Availability

Academic Institutions and Nonprofits only

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pTRE3G-NlucP

Plasmid

#162595

Purpose

C-terminally PEST-tagged NanoLuc under TRE3G promoter

Depositor

Masaharu Somiya

Article

Somiya et al J Extracell Vesicles. 2021 Nov;10(13):e1

Insert

NanoLuc-PEST

Tags

PEST

Expression

Mammalian

Promoter

TRE3G promoter

Available Since

Feb. 18, 2021

Availability

Academic Institutions and Nonprofits only

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