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pAW8ENdeI-c3HA-spo5DSR

Plasmid

#49872

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with the S. pombe spo5 gene DSR element into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-6XDSR

Plasmid

#49870

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 6 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-3XDSR

Plasmid

#49868

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 3 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-8XDSR

Plasmid

#49866

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-cyEGFP-4XDSR

Plasmid

#49864

Purpose

for Cre-lox cassette exchange of C-terminal yEGFP tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

yEGFP

Available Since

Dec. 20, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-8XDSR

Plasmid

#49871

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 8 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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pAW8ENdeI-c3HA-4XDSR

Plasmid

#49869

Purpose

for Cre-lox cassette exchange of C-terminal 3XHA tagged gene sequences together with 4 copies of the core DSR motif into the S. pombe urg1 locus

Depositor

Tony Carr

Article

Watson et al PLoS One. 2013 Dec 20;8(12):e83800. doi:

Type

Empty backbone

Use

Cre/Lox

Tags

3XHA

Available Since

Dec. 13, 2013

Availability

Academic Institutions and Nonprofits only

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KaiC CII cat-/EE (E318Q; S431E; T432E)

Plasmid

#42498

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

KaiC CII cat-/EE (E318Q; S431E; T432E)

Expression

Bacterial

Mutation

E318Q; S431E; T432E

Promoter

t7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
KaiC CI cat- (E77Q;E78Q)

Plasmid

#41886

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

KaiC CI cat -(E77Q;E78Q)

Expression

Bacterial

Mutation

changed glutamic acid 77 and 78 to glutamine

Promoter

t7 promoter

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
KaiC CII cat- (E318Q)

Plasmid

#41887

Depositor

Michael Rust

Article

Phong et al Proc Natl Acad Sci U S A. 2013 Jan 15;11

Insert

Kai C CII cat (E318Q)

Use

Bl de3 21

Expression

Bacterial

Mutation

changed amino acid 318 E to Q

Promoter

t7

Available Since

March 4, 2013

Availability

Academic Institutions and Nonprofits only
RcP9

Plasmid

#242157

Purpose

Recombineering Plasmid. ITPG-induced expression of Recombitron operon (ccdB dropout cassette in Eco1 Retron); PSP-Tet promoter for Gene III induction upon M13 phage infection and aTc addition.

Depositor

Dominik Niopek

Article

Southern et al Nat Commun. 2026 Apr 14;17(1):3498. doi:

Type

Empty backbone

Expression

Bacterial

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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pGSKU

Plasmid

#72243

Purpose

Contains the CORE cassette with convergent KlURA3 and KanMX4 markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
kanMX4
I-SceI

Expression

Bacterial

Available Since

Jan. 28, 2016

Availability

Academic Institutions and Nonprofits only

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pGSHU

Plasmid

#72244

Purpose

Contains the CORE cassette with convergent KlURA3 and hyg markers and the I-SceI gene under the inducible GAL1 promoter

Depositor

Michael Resnick, Francesca Storici

Article

Storici et al Proc Natl Acad Sci U S A. 2003 Dec 9;100

Inserts

KlURA3
hyg
I-SceI

Expression

Bacterial

Available Since

Jan. 29, 2016

Availability

Academic Institutions and Nonprofits only

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JC201: pMVP/Ad/5-35-DEST

Plasmid

#121847

Purpose

pMVP destination vector, empty 5/35 fiber-modified adenovirus vector backbone

Depositor

Christopher Newgard

Article

Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

Type

Empty backbone

Use

Adenoviral; Pmvp gateway destination vector

Available Since

June 27, 2019

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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AAVS1-idCas9-vpr

Plasmid

#89985

Purpose

inducible CRISPR-ON system for controllable gene activation; this plasmid is used to insert dCas9-VPR casette in one allele of AAVS1 locus

Depositor

Jie Na

Article

Guo et al Protein Cell. 2017 Jan 23. doi: 10.1007/

Insert

dCas9-VPR

Expression

Mammalian

Promoter

TRE-tight

Available Since

June 6, 2017

Availability

Academic Institutions and Nonprofits only

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pCAG-loxPSTOPloxP-ZsGreen

Plasmid

#51269

Purpose

Cre-responsive fluorescent reporter plasmid containing a STOP-cassette flanked by loxP sites followed by ZsGreen

Depositor

Pawel Pelczar

Article

Hermann et al Nucleic Acids Res. 2014 Jan 9.

Insert

loxP-STOP-loxP-ZsGreen

Expression

Mammalian

Promoter

CAG

Available Since

March 17, 2014

Availability

Academic Institutions and Nonprofits only

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pCI SMN2

Plasmid

#72287

Purpose

SMN2 exon 7 splicing cassette

Depositor

Elliot Androphy

Article

Lorson et al Proc Natl Acad Sci U S A. 1999 May 25;96

Insert

SMN2 (SMN2 Human)

Expression

Mammalian

Mutation

contains exons 6-7-8 including introns, multiple …

Promoter

CMV

Available Since

Jan. 11, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-BbsI-PITCh

Plasmid

#127875

Purpose

For user to clone their gRNA oligos to BbsI site. PITCh gRNA expression cassette is already build in so there is no need to perform Golden Gate assembly

Depositor

Peter Kaiser

Article

Lin et al J Biol Chem. 2019 May 28. pii: RA119.008

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Available Since

July 8, 2019

Availability

Academic Institutions and Nonprofits only

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pLC-ZsGreen-P2A-Puro

Plasmid

#124302

Purpose

Lentiviral vector for expression of ZsGreen-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood Adv. 2019 Jul 23;3(14):2105-2117.

Insert

ZsGreen

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 22, 2019

Availability

Academic Institutions and Nonprofits only

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