19,658 results

1. pcDNA3.1-CHRNA7

   Plasmid

   #62276

   Purpose

   mammalian expression of human Alpha7 (CHRNA7)

   Depositor

   Sherry Leonard, Henry Lester

   Article

   Wang et al J Biol Chem. 2014 Sep 19;289(38):26451-6

   Insert

   CHRNA7 (CHRNA7 Human)
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   May 21, 2015

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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2. EFSp-GFP-YAP (5SA)

   Plasmid

   #174170

   Purpose

   Lentiviral vector to constitutively express overactive YAP (5 LATS phosporylation sites mutated to Ala) under control of the EFS promoter.

   Depositor

   Rod Bremner

   Article

   Pearson et al Cancer Cell. 2021 Jul 13. pii: S1535-610

   Insert

   YAP (YAP1 Human)
   

   Use

   Lentiviral

   Tags

   3x FLAG tag

   Expression

   Mammalian

   Mutation

   Five LATS phosphorylation sites (S61, S109, S127,…

   Promoter

   EFS

   Available Since

   Sept. 28, 2021

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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3. pAAV-hSyn1-mNeonGreen

   Plasmid

   #99135

   Purpose

   An AAV genome that ubiquitously expresses the fluorescent protein mNeonGreen from the hSyn1 promoter

   Depositor

   Viviana Gradinaru

   Article

   Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

   Insert

   mNeonGreen
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   hSyn1

   Available Since

   March 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. pBCW

   Plasmid

   #62658

   Purpose

   Mammalian expression of PhiC31o recombinase from the broadly active CAG promoter/enhancer

   Depositor

   Masato Ohtsuka

   Article

   Ohtsuka et al BMC Genomics. 2015 Apr 9;16:274. doi: 10

   Insert

   PhiC31
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 24, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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5. lenti EF1a-FLAG-dCas9-VPR

   Plasmid

   #114195

   Purpose

   Lentivirus compatible dCas9-VPR construct driven by the EF1a promoter

   Depositor

   Jeremy Day

   Article

   Savell et al eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-049

   Insert

   dCas9-VPR
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGL-FLKif3A

   Plasmid

   #13742

   Depositor

   Linda Wordeman

   Article

   Ginkel et al Mol Biol Cell. 2000 Dec . 11(12):4143-58

   Insert

   mouse Kif3A (Kif3a Mouse)
   

   Use

   Cre/Lox

   Expression

   Mammalian

   Available Since

   Jan. 29, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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7. pHybrid-Electra2

   Plasmid

   #179475

   Purpose

   Expresses Electra2 in bacterial cells and Electra2 and mScarlet in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Inserts

   Electra2
   mScarlet-I
   

   Expression

   Bacterial and Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pTarget_CAST

   Plasmid

   #127926

   Purpose

   Target plasmid containing PSP1

   Depositor

   Feng Zhang

   Article

   Strecker et al Science. 2019 Jul 5;365(6448):48-53. doi

   Insert

   PSP1 target (GGTT PAM)
   

   Available Since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. lenti-25xAARE-minP-EGFP

   Plasmid

   #159666

   Purpose

   EGFP reporter plasmid containing 25 tandem repeats of the amino acid response element (AARE)

   Depositor

   Seiichi Oyadomari

   Article

   Kitakaze et al Elife. 2019 Dec 17;8. pii: 43302. doi: 1

   Insert

   25xAARE-minP-EGFP
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   March 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. GFP-Dcp1b

    Plasmid

    #153976

    Purpose

    Fluorescent protein fused to mRNA decapping enzyme 1b

    Depositor

    Gia Voeltz

    Article

    Lee et al Science. 2020 Jan 31;367(6477). pii: 367

    Insert

    mRNA recapping enzybe 1b (DCP1B Human)
    

    Tags

    pAcGFP-C1

    Expression

    Mammalian

    Available Since

    July 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pCAG-EGFP(HiBiT)

    Plasmid

    #162589

    Purpose

    N-terminally HiBiT-tagged EGFP under CAG promoter

    Depositor

    Masaharu Somiya

    Article

    Somiya et al Anal Chem. 2021 Mar 24. doi: 10.1021/acs

    Insert

    EGFP
    

    Tags

    HiBiT

    Expression

    Mammalian

    Promoter

    CAG promoter

    Available Since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. CMV-G-GECO1.0

    Plasmid

    #32447

    Purpose

    Mammalian expression of green fluorescent genetically encoded Ca2+ indicator for optical imaging (version 1.0)

    Depositor

    Robert Campbell

    Article

    Zhao et al Science. 2011 Sep 30;333(6051):1888-91.

    Insert

    G-GECO1.0
    

    Expression

    Mammalian

    Mutation

    GCaMP3 K119I/L173Q/S404G/E430V

    Available Since

    Sept. 29, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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13. pJR101

    Plasmid

    #187241

    Purpose

    Lentiviral sgRNA vector for Perturb-seq with mU6 sgRNA promoter, CR1 constant region with CS1 capture sequence in stem loop, and UCOE EF1alpha driving PURO-GFP marker expression

    Depositor

    Marco Jost, Jonathan Weissman

    Article

    Replogle et al eLife. 2022 Dec 28;11:e81856. doi: 10.75

    Insert

    Lentiviral sgRNA vector for Perturb-seq with mU6 promoter
    

    Use

    Lentiviral

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. pKLV2.2-h7SKgRNA5(SapI)-hU6gRNA5(BbsI)-PGKpuroBFP-W

    Plasmid

    #72666

    Purpose

    Lentiviral dual CRISPR gRNA expression vector (human 7SK and human U6 promoters)

    Depositor

    Kosuke Yusa

    Article

    Tzelepis et al Cell Rep. 2016 Oct 18;17(4):1193-1205. d

    Insert

    h7SKgRNA cassette, hU6gRNA cassette, PGKpuroBFP cassette, WPRE
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Available Since

    Oct. 6, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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15. PKC eta DN

    Plasmid

    #21246

    Depositor

    Bernard Weinstein

    Article

    Soh et al J Biol Chem. 2003 Sep 5. 278(36):34709-1

    Insert

    PKC eta (Prkch Mouse)
    

    Tags

    HA

    Expression

    Mammalian

    Mutation

    K384R

    Available Since

    Aug. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

16. pET28a-FIP3

    Plasmid

    #74753

    Purpose

    Expressed sfGFP-tagged FIP3 in E. coli for protein production

    Depositor

    Ron Vale

    Article

    Schroeder et al Elife. 2014 Oct 1;3:e03351. doi: 10.7554

    Insert

    Rab11 family interacting protein 3 (RAB11FIP3 Human)
    

    Tags

    6x His, Strep II, and superfolder GFP

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    June 13, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pRCC-K

    Plasmid

    #81191

    Purpose

    Expression of Cas9 and gRNA cassette in S. cerevisiae; Kan Resistance

    Depositor

    Eckhard Boles

    Article

    Generoso et al J Microbiol Methods. 2016 Aug;127:203-5.

    Inserts

    Cas9
    empty gRNA cassette
    

    Use

    CRISPR

    Tags

    NTS

    Expression

    Yeast

    Mutation

    Please see depositor comments below. and WT - cod…

    Promoter

    ROX3 and SNP52p

    Available Since

    Sept. 8, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. AAV-EF1a-DIO-GCaMP6f-P2A-nls-dTomato

    Plasmid

    #51083

    Purpose

    Cre dependent expression of GCaMP6f Ca sensor and physically separate nuclear localized dTomato fluorophore

    Depositor

    Jonathan Ting

    Has Service

    AAV Retrograde

    Insert

    GCaMP6f
    

    Use

    AAV

    Tags

    P2A-nls-dTomato

    Expression

    Mammalian

    Promoter

    hEF1a

    Available Since

    Feb. 27, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. TRE-KRAB-dCas9-IRES-GFP

    Plasmid

    #85556

    Purpose

    2nd generation lentiviral vector expressing a KRAB-dCas9 fusion protein and GFP

    Depositor

    Eric Lander

    Article

    Fulco et al Science. 2016 Sep 29. pii: aag2445.

    Insert

    KRAB-dCas9-IRES-GFP
    

    Use

    CRISPR and Lentiviral

    Tags

    2x NLS, HA Tag, and KRAB domain

    Expression

    Mammalian

    Promoter

    TRE3G

    Available Since

    Sept. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pRK2-AraE

    Plasmid

    #110141

    Purpose

    Constitutive promoter expressing araE on RK2 origin

    Depositor

    Brian Pfleger

    Article

    Cook et al J Ind Microbiol Biotechnol. 2018 Jan 3.

    Insert

    araE
    

    Expression

    Bacterial

    Available Since

    June 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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