We narrowed to 17,362 results for: JUN

1. piggybac-Syn-mScn8a-3xV5

   Plasmid

   #182554

   Purpose

   Expresses Nav1.6 tagged with 3xV5

   Depositor

   James Zhe Liu

   Article

   Liu et al J Neurosci. 2022 Jun 6;42(28):5482-98. d

   Insert

   Scn8a (Scn8a Mouse)
   

   Use

   Tags

   3xV5

   Expression

   Mammalian

   Mutation

   Promoter

   Syn

   Available since

   March 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pN256

   Plasmid

   #188472

   Purpose

   scDAISY-seq lentiviral construct encoding crRNA targeting the DAISY chain that is within the UTR of eGFP

   Depositor

   Le Cong

   Article

   Hughes et al Mol Cell. 2022 Jun 22. pii: S1097-2765(2

   Insert

   eGFP-WPRE-DAISY-4T
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available since

   Sept. 6, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAsCpf1(TYCV)(BB) (pY211)

   Plasmid

   #89352

   Purpose

   Expresses humanized AsCpf1 TYCV PAM variant and crRNA guide

   Depositor

   Feng Zhang

   Article

   Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

   Insert

   Cpf1, RR variant
   

   Use

   CRISPR

   Tags

   NLS and NLS-3xHA

   Expression

   Mammalian

   Mutation

   S542R/K607R

   Promoter

   CBh

   Available since

   July 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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4. pEG302_dCAS9-MQ1(Q147L)_g4+g10+g18

   Plasmid

   #172316

   Purpose

   CRISPR dCas9 directly fused to a variant of bacterial DNA methyltransferase MQ1 to target CG specific methylation to the FWA locus with three guide RNAs

   Depositor

   Steven Jacobsen

   Article

   Ghoshal et al Proc Natl Acad Sci U S A. 2021 Jun 8;118

   Insert

   g18_U6_g10_U6_g4_U6_UBQ10_Ω_dCas9_MQ1(Q147L)_OCS
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available since

   Sept. 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pFastBac1-OSF-TRIM5alpha(human)

   Plasmid

   #79034

   Purpose

   Baculoviral entry vector to produce Bac-to-bac baculoviruses

   Depositor

   Wesley Sundquist

   Article

   Li et al Elife. 2016 Jun 2;5. pii: e16269. doi: 1

   Insert

   OSF-TRIM5alpha(human) (TRIM5 Human)
   

   Use

   Tags

   OneSTrEP-FLAG

   Expression

   Insect

   Mutation

   Promoter

   polyhedrin

   Available since

   March 3, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pBM019

   Plasmid

   #128179

   Purpose

   Construct for generating stable Cas9-expressing Toxoplasma gondii

   Depositor

   Sebastian Lourido

   Article

   Markus et al mSphere. 2019 Jun 26;4(3). pii: 4/3/e003

   Inserts

   sgRNA#1
   SpCas9
   Chloramphenicol acetyltransferase
   

   Use

   CRISPR; Toxoplasma gondii expression

   Tags

   3X FLAG and Nuclear Localization Signal

   Expression

   Mutation

   Promoter

   TUB1 (Toxoplasma gondii), U6 (Toxoplasma gondii),…

   Available since

   July 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-CAG-EBFP2-P2A-EGFP

   Plasmid

   #184938

   Purpose

   Expresses blue fluorescent protein EBFP2 and EGFP in mammalian cells

   Depositor

   Kiryl Piatkevich

   Article

   Papadaki et al Sci Rep. 2022 Jun 17;12(1):10190. doi: 1

   Insert

   EBFP2-P2A-EGFP
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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8. pHR LAT-FFF/Zap70 iLID-Drop

   Plasmid

   #171032

   Purpose

   Light-induced LAT-Zap70 dimerization and LAT clustering. Zap70 is TagRFP-tagged. LAT has three Tyr-Phe mutations.

   Depositor

   Jared Toettcher

   Article

   Dine et al Cell Rep. 2021 Jun 22;35(12):109280. doi

   Insert

   LAT (LAT Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Sept. 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pcDNA3-Nrxn1beta AS4(-)-Fc

   Plasmid

   #59313

   Purpose

   Expression plasmid for secreted Fc-fusion of mouse Neurexin 1beta AS4(-) [lacking the insert at alternatively spliced segment 4 (AS4-)]

   Depositor

   Tito Serafini, Peter Scheiffele

   Article

   Scheiffele et al Cell. 2000 Jun 9. 101(6):657-69.

   Insert

   Nrxn1beta AS4(-)-Fc fusion (Nrxn1 Mouse)
   

   Use

   Tags

   Fc (from human IgG)

   Expression

   Mammalian

   Mutation

   Promoter

   CMV, T7

   Available since

   June 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-CRY2(D387A)-mCherry

    Plasmid

    #58369

    Purpose

    Light insensitive CRY2 mutant

    Depositor

    Won Do Heo

    Article

    Lee et al Nat Methods. 2014 Jun;11(6):633-6. doi:

    Insert

    CRY2[1-498] (D387A) (CRY2 Mustard Weed)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    D387A

    Promoter

    CMV

    Available since

    Sept. 30, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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11. BPK2301

    Plasmid

    #65778

    Purpose

    Human expression plasmid for S. thermophilus 1 Cas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-St1-sgRNA

    Depositor

    Keith Joung

    Article

    Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

    Insert

    St1Cas9 gRNA backbone, without spacer sequence
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    June 22, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG-Bmpr2Δ751-813aa

    Plasmid

    #163603

    Purpose

    Expresses BMPR-2 lacking the LIMK-binding region in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The LIMK-binding region (751-813 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-Bmpr2ΔKinase

    Plasmid

    #163404

    Purpose

    Expresses BMPR-2 lacking the kinase domain in mammalian cells

    Depositor

    Takeshi Imai

    Article

    Aihara et al Cell Rep. 2021 Jun 22;35(12):109276. doi

    Insert

    Bmpr2 (Bmpr2 Mouse)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    The kinase domain (202-500 aa) is deleted

    Promoter

    Available since

    Aug. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAsCpf1(TATV)(BB) (pY221)

    Plasmid

    #89354

    Purpose

    Expresses humanized AsCpf1 TATV PAM variant and crRNA guide

    Depositor

    Feng Zhang

    Article

    Gao et al Nat Biotechnol. 2017 Jun 5. doi: 10.1038

    Insert

    Cpf1 RVR variant
    

    Use

    CRISPR

    Tags

    NLS and NLS-3xHA

    Expression

    Mammalian

    Mutation

    S542R/K548V/N552R

    Promoter

    CBh

    Available since

    July 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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15. pMXs-IP GFP-Omp25

    Plasmid

    #38249

    Depositor

    Noboru Mizushima

    Article

    Yoshii et al J Biol Chem. 2011 Jun 3;286(22):19630-40

    Insert

    Omp25 (Synj2bp Rat)
    

    Use

    Retroviral

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    plasmid contains AA109-145 of rat Omp25/Synj2bp

    Promoter

    Available since

    Sept. 2, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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16. LentiU6-hASCL1 gRNA_1-MS2-Puro

    Plasmid

    #192670

    Purpose

    Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription

    Depositor

    Jeffrey Miner

    Article

    Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. do

    Insert

    Human ASCL1 activating gRNA #1 (ASCL1 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    U6

    Available since

    Dec. 15, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCMV-CMP-PE-V1

    Plasmid

    #202769

    Purpose

    Expresses CMP-PE-V1 in mammalian cells for prime editing

    Depositor

    Kyoungmi Kim

    Article

    Park et al Genome Biol. 2021 Jun 3;22(1):170. doi:

    Insert

    CMP-PE-V1
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCDNA3 Flag MKK7b1(glu)

    Plasmid

    #14673

    Depositor

    Roger Davis

    Article

    Tournier et al Mol Cell Biol. 1999 Feb . 19(2):1569-81.

    Insert

    MKK7b1(Glu) (Map2k7 Mouse)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Ser-269Glu Thr-273Glu

    Promoter

    Available since

    July 25, 2007

    Availability

    Academic Institutions and Nonprofits only

19. pRK5-myc-Miro1 K427V

    Plasmid

    #47892

    Purpose

    Expresses myc tagged Miro1 K427 constitutively active mutant

    Depositor

    Pontus Aspenström

    Article

    Fransson et al Biochem Biophys Res Commun. 2006 Jun 2;3

    Insert

    Miro1 K427V (RHOT1 Human)
    

    Use

    Tags

    myc

    Expression

    Mammalian

    Mutation

    K427V, constitutively active mutant

    Promoter

    CMV

    Available since

    Sept. 12, 2013

    Availability

    Academic Institutions and Nonprofits only

20. shCDK4/6(1)

    Plasmid

    #73554

    Purpose

    vector encoding both shRNA against CDK4(1) and shRNA against CDK6(1)

    Depositor

    Gerardo Ferbeyre

    Article

    Acevedo et al Cancer Res. 2016 Jun 1;76(11):3252-64. d

    Insert

    shRNA against CDK4 + shRNA against CDK6
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    LTR

    Available since

    Aug. 8, 2016

    Availability

    Academic Institutions and Nonprofits only