We narrowed to 17,662 results for: por
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Plasmid#133881Purpose2 copies of the p53 DNA binding motifDepositorInsert2 copies of the p53 DNA binding motif
UseLuciferase and Synthetic BiologyExpressionMammalianAvailable SinceJune 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCIneo-EloC
Plasmid#31982DepositorInsertElongin C (ELOC Human)
ExpressionMammalianAvailable SinceJuly 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pPD3C
Plasmid#86971PurposeExpresses PCNA3 protein fused phosphite dehydrogenase and P450cam in E. coliDepositorInsertPCNA3 fused to phosphite dehydrogenase and P450cam
TagsHis6 tagExpressionBacterialMutationE175A/A176R in PTDH, N106R in PCNA3, silent mutat…PromoterT7 promoterAvailable SinceMarch 15, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-ERKsubDD-Venus-NES
Plasmid#84630PurposeEncodes C-terminal (substrate) fragment of bimolecular ERK activity reporter (bimEKAR); cytosol targetedDepositorInsertERKsubDD-Venus-NES
Tags6xHis, Nuclear export signal (NES), T7 tag (gene …ExpressionMammalianPromoterCMVAvailable SinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only -
pGREAT4
Plasmid#170892PurposeGoldenBraid Double Transcriptional Unit - pG10-90_E-Luc_NOSt+pNOS_Red-F_NOStDepositorInsertE-Luc
UseLuciferase and Synthetic BiologyExpressionPlantMutationc.375C>T silent mutation to remove BbsI site (…PromoterpG10-90Available SinceAug. 11, 2021AvailabilityAcademic Institutions and Nonprofits only -
pWZL N-Myc-hPOT1_IRES_hygro
Plasmid#166406Purposeexpress hPOT1 in mammalian cellsDepositorAvailable SinceApril 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
pPN144
Plasmid#91673PurposeExpress sgRNA targeting human TBC1D5DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN145
Plasmid#91674PurposeExpress sgRNA targeting human TBC1D5DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET22b-ecDHFR-Y100F
Plasmid#110826PurposeBacterial expression of E.coli DHFR Y100F mutantDepositorInsertecDHFR
ExpressionBacterialMutationY100 mutated to F100PromoterT7Available SinceJune 7, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAL950
Plasmid#119748Purposeprecursor outer membrane pore protein E, phoE with mutations Alanine 249 to Cysteine and Glutamine 307 to CysteineDepositorInsertprecusor Outer membrane pore protein E, phoE, with mutations Alanine 249 to Cysteine and Glutamine 307 to Cysteine
TagsnoneExpressionBacterialMutationInternal NdeI site removed and mutations Alanine …PromoterT7Available SinceJan. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pPN216
Plasmid#91675PurposeExpress sgRNA targeting human TLE1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-RLuc8C-FHA1-NES
Plasmid#138210PurposeEncodes PAABD fragment of luminescence-based bimolecular kinase activity reporters (LumKARs); cytosol targeted.DepositorInsertRLuc8C-FHA1-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-mCherry-PKAsub-RLuc8N-NES
Plasmid#138211PurposeEncodes substrate fragment of luminescence-based bimolecular PKA activity reporter (LumAKAR); cytosol targeted. Use in conjunction with pcDNA3-RLuc8C-FHA1-NES.DepositorInsertmCherry-PKAsub-RLuc8N-NES
Tags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianPromoterCMVAvailable SinceJan. 25, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-h-mmPtch1-B-HA
Plasmid#120902PurposeExpresses Ptch1-B for function assaysDepositorInsertptch1 (Ptch1 Mouse)
TagsHAExpressionMammalianMutationdeleted amino acids 620-643, truncated at 1291PromoterCMVAvailable SinceJan. 17, 2019AvailabilityAcademic Institutions and Nonprofits only -
PcDNA-DEST53-CypB-Nucl N-term GFP
Plasmid#36123DepositorInsertcyclophilin B (PPIB Human, contains CypB AA 33-216)
TagsGFPExpressionMammalianMutationA212SPromoterCMVAvailable SinceNov. 1, 2012AvailabilityAcademic Institutions and Nonprofits only -
pPN023
Plasmid#91671PurposeExpress sgRNA targeting human SYNGAP1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pPN024
Plasmid#91672PurposeExpress sgRNA targeting human SYNGAP1DepositorAvailable SinceOct. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
Rae1-pDONR221
Plasmid#124240PurposeGateway entry clone of Drosophila Rae1 cDNA.DepositorInsertDrosophila Rae1 (Rae1 Fly)
ExpressionInsectAvailable SinceApril 9, 2019AvailabilityAcademic Institutions and Nonprofits only -
pIhh715bp-Luc
Plasmid#53606PurposeReporter construct containing a 715 bp promoter region of the Ihh gene in front of a luciferase gene for in vitro DNA transfection assaysDepositorInsertIhh promoter
UseLuciferase; ReporterAvailable SinceJune 23, 2014AvailabilityAcademic Institutions and Nonprofits only -
A3H HapI-Cas9n-UGI
Plasmid#119140PurposeBase editor made from APOBEC3H Haplotype IDepositorInsertAPOBEC3H Haplotype I-Cas9 nickase-UGI
UseCRISPRMutationNonePromoterCMVAvailable SinceJune 10, 2019AvailabilityAcademic Institutions and Nonprofits only