4,183 results

1. nls-NgAgo-GK

   Plasmid

   #78253

   Purpose

   Mammalian expression of NgAgo (Argonaute from N. gregoryi).

   Depositor

   Chunyu Han

   Article

   Gao et al Nat Biotechnol. 2016 May 2. doi: 10.1038

   Insert

   NgAgo
   

   Tags

   a nuclear localization signal (NLS)

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   June 2, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKH3-human RSK1

   Plasmid

   #13841

   Depositor

   John Blenis

   Article

   Richards et al Mol Cell Biol. 2001 Nov . 21(21):7470-80

   Insert

   RSK1 (RPS6KA1 Human)
   

   Tags

   3x HA

   Expression

   Mammalian

   Available Since

   Jan. 25, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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3. pdCas9-DNMT3A-PuroR

   Plasmid

   #71667

   Purpose

   Expression of human codon-optimized dCas9-DNMT3A fusion with T2A-PuroR for targeted DNA methylation in mammalian cells; cloning backbone for sgRNA

   Depositor

   Vlatka Zoldos

   Article

   Vojta et al Nucleic Acids Res. 2016 Mar 11. pii: gkw

   Insert

   S. pyogenes dCas9 fused with the catalytic domain of human DNMT3A (amino acids P602-V912) and T2A-PuroR (DNMT3A S. pyogenes, Synthetic, Human)
   

   Use

   CRISPR

   Tags

   3xFLAG, SV40 NLS, and T2A-PuroR

   Expression

   Mammalian

   Mutation

   D10A and H840A in S.pyogenes Cas9

   Promoter

   CBh

   Available Since

   March 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-Casp3 C163A-myc

   Plasmid

   #11814

   Depositor

   Guy Salvesen

   Article

   Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

   Insert

   Caspase 3 catalytic mutant (CASP3 Human)
   

   Tags

   Myc

   Expression

   Mammalian

   Mutation

   C163A

   Available Since

   July 19, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pEGFP-C1-PKM2

   Plasmid

   #64698

   Purpose

   Expression of GFP tagged PKM2 in mammalian cells

   Depositor

   Axel Ullrich

   Article

   Stetak et al Cancer Res. 2007 Feb 15;67(4):1602-8.

   Insert

   PKM2 (PKM Human)
   

   Tags

   GFP

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Nov. 7, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. pAAV-FLEX-ArchT-tdTomato

   Plasmid

   #28305

   Purpose

   Cre-dependent viral expression of ArchT-tdTomato for neuronal inhibition

   Depositor

   Edward Boyden

   Article

   Han et al Front Syst Neurosci. 2011;5:18. Epub 201

   Has Service

   AAV5

   Insert

   ArchT-tdTomato
   

   Use

   AAV and Cre/Lox; Adeno-associated virus

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available Since

   Aug. 25, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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7. 1245 pCMVb p300

   Plasmid

   #10717

   Depositor

   William Sellers

   Insert

   p300 (EP300 Human)
   

   Expression

   Mammalian

   Available Since

   Dec. 7, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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8. Rab5a-pmCherryC1

   Plasmid

   #27679

   Depositor

   Christien Merrifield

   Article

   Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

   Insert

   Rab5a (Rab5a Mouse)
   

   Tags

   mCherry

   Expression

   Mammalian

   Available Since

   May 4, 2011

   Availability

   Academic Institutions and Nonprofits only

   

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9. pMSCV-IRES-CFP II (pMIC II)

   Plasmid

   #52109

   Purpose

   a bicistronic IRES-CFP containing retroviral vector, MCS is expanded from pMSCV-IRES-GFP

   Depositor

   Dario Vignali

   Article

   Holst et al Nat Protoc. 2006;1(1):406-17.

   Type

   Empty backbone

   Use

   Retroviral

   Tags

   IRES-ECFP

   Expression

   Mammalian

   Available Since

   April 9, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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10. mCherry-UtrCH

    Plasmid

    #26740

    Depositor

    William Bement

    Article

    Burkel et al Cell Motil Cytoskeleton. 2007 Nov . 64(1

    Insert

    Utrophin
    

    Use

    Reporter for f-actin

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Calponin homology domain of utrophin (aa# 1-261)

    Available Since

    March 18, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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