We narrowed to 7,283 results for: Tet-on
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Plasmid#233294PurposeIntegration of msfGFP (or your Module Of Interest) in a random fashion via Tn5 insertion. The antibiotic cassette used for selecting for the integration can be removed in a later stage with pFNC.DepositorInsertmsfGFP
Promoterp14G-BCD2Available SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
pFNC-5
Plasmid#233297PurposePlasmid encoding the BxbI phage integrase. It can be used to remove the antibiotic resistance cassette integrated with pCIFR. TcR, easily curable via sucrose counterselection.DepositorInsertsBxbI phage integrase
sacB
ExpressionBacterialPromoterPEM7 and unknownAvailable SinceApril 9, 2025AvailabilityAcademic Institutions and Nonprofits only -
Bxb1-GA_CAG-rtTA-T2A-mTagBFP2
Plasmid#229797PurposeBxb1-GA donor plasmid for constitutive expression of reverse tetracycline transactivator (rtTA) and nuclear mTagBFP2DepositorInsertrtTA-T2A-mTagBFP2
UseSynthetic BiologyTagsNLSExpressionMammalianPromoterCAGAvailable SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-crBFP_EF1a-BFP
Plasmid#224785PurposeBFP targeting crRNA for RfxCas13d expressed from hU6 promoter and target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6 and hU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC-hU6-CrEGFP_EF1a-BFP
Plasmid#224786PurposeEGFP targeting crRNA for RfxCas13d expressed from hU6 promoter and non-target BFP protein expressed from EF1a promoterDepositorInsertcrBFP
UseCRISPR and Synthetic BiologyExpressionMammalianPromoterhU6 and hU6-2xTetOAvailable SinceOct. 31, 2024AvailabilityAcademic Institutions and Nonprofits only -
AA440
Plasmid#216036PurposeFragmid fragment: (Cas protein) binds to TRE in absence of doxycyclineDepositorHas ServiceCloning Grade DNAInsertTetR_v1.1
UseCRISPR; FragmentAvailable SinceApril 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYG215
Plasmid#200837PurposeTranscribes a glmZ' (1-146)-gfp fusion. gfp can be replaced via AgeI/XbaI-sites with gene of interest to release its RNA with a 5' monophosphate upon cleavage.DepositorInsertglmZ (glmZ Escherichia coli str. K-12 substr. MG1655)
UseSynthetic BiologyTagsGFPExpressionBacterialPromoterP-LlacO-1Available SinceAug. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
DsRed -7e
Plasmid#191765PurposeExpression of DsRed fused to a negatively charged polypeptide. Overall charge on tetramer: -7eDepositorInsertDsRed
TagsN terminal fusion of negatively charged polypetod…ExpressionBacterial and MammalianPromoterCMVAvailable SinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
CMV+ (mScarlet-I) plasmid with onboard mCitrine cassette
Plasmid#169743PurposeCMV+ circuit plasmid that encodes mScarlet-I to report the circuit output levels. This plasmid also encodes a separate mCitrine expression cassette to monitor plasmid dosage.DepositorInsertmScarlet-I
UseSynthetic BiologyExpressionMammalianPromoterpCMV-tetO2Available SinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_Kctd19-3xHA
Plasmid#173497PurposeExpress Kctd19-3xHA under the mouse Clgn promoter.DepositorInsertPotassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
TagsHAExpressionMammalianAvailable SinceSept. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
pClgn1.1_3xFLAG-Kctd19
Plasmid#173498PurposeExpress 3xFLAG-Kctd19 under the mouse Clgn promoter.DepositorInsertPotassium Channel Tetramerization Domain Containing 19 (Kctd19 Mouse)
TagsFLAGExpressionMammalianAvailable SinceSept. 10, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-SQSfl
Plasmid#171010PurposeHiLITR protease with full-length SQS targeting information (ER)DepositorInsertEGFP-uTEV1-SQS(FL)
UseLentiviral and Synthetic BiologyExpressionMammalianPromoterpTRE-tight (TetOn)Available SinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
RA35
Plasmid#141126PurposeExpresses dimeric LacI repressor (LacI-11) under Rhl promoterDepositorInsertsTagsssrA degradation tagExpressionBacterialMutationtruncated/dimeric version of LacI (missing last 1…Promoterengineered rhl promoter (responds to C4-HSL) and …Available SinceJuly 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCI-neo-His-MS2BP-G3BP1-S149A
Plasmid#136009PurposeS149A G3BP1 inserted with MS2BP tagged on the N-terminus to use with the Tethering Assay (MS2)DepositorAvailable SinceFeb. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380E
Plasmid#100035PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 533 nm.DepositorInsertRhoGC D380E
Tags1D4 (TETSQVAPA)ExpressionMammalianMutationD380EPromoterAdenovirus major late promoterAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pMT3-RhoGC D380N
Plasmid#100034PurposeThis plasmid is for expression of a rhodopsin/guanylyl cyclase fusion protein mutant with lambda max = 506 nm.DepositorInsertRhoGC D380N
Tags1D4 (TETSQVAPA)ExpressionMammalianMutationD380NPromoterAdenovirus major late promoterAvailable SinceSept. 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pZS1[Iq:LacI-T]
Plasmid#60756PurposeContains PIq driving expression LacI-T, the Lactose inducible chimera with the TAN DBD.DepositorInsertLacI-T
UseSynthetic BiologyExpressionBacterialMutationLacI without the tetramerization domain and the T…Available SinceJune 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pFAB1907
Plasmid#47857PurposeBIOFAB reporter plasmid for measuring BBa_B1006 U10 termination efficiencyDepositorInsertBBa_B1006 U10
UseSynthetic BiologyExpressionBacterialMutationAdded two extra U at the end of Bba_1006 poly-U t…PromoterpLTetO12Available SinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pFAB801
Plasmid#47846PurposeBIOFAB reporter plasmid for measuring trp_att_L126 termination efficiencyDepositorInserttrp_att_L126 terminator
UseSynthetic BiologyExpressionBacterialMutationLoop mutation in trp TerminatorPromoterpLTetO1Available SinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
CMV-sgE1TSS-short-3'box
Plasmid#92165PurposesgRNA expression vector for Evx1as RNA tethering assayDepositorInsertEvx1as short isoform
UseCRISPRPromoterCMVAvailabilityAcademic Institutions and Nonprofits only