We narrowed to 14,241 results for: cas9 genes

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BPK1520

Plasmid

#65777

Purpose

Human expression plasmid for SpCas9 sgRNA (need to clone in spacer into BsmBI sites): U6-BsmBIcassette-Sp-sgRNA

Depositor

Keith Joung

Article

Kleinstiver et al Nature. 2015 Jun 22. doi: 10.1038/nature

Insert

SpCas9 gRNA backbone, without spacer sequence

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

June 22, 2015

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-DsRed-P2A-Bsr

Plasmid

#83919

Purpose

Lentiviral SpCas9-gRNA expression vector with DsRed-Express2-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

DsRed-Express2
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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sgRNA(MS2) cloning backbone

Plasmid

#61424

Purpose

sgRNA cloning backbone with MS2 loops at tetraloop and stemloop 2. Contains BbsI sites for insertion of spacer sequences.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2014 Dec 10. doi: 10.1038/nature

Has Service

Cloning Grade DNA

Type

Empty backbone

Use

CRISPR

Expression

Mammalian

Promoter

U6

Available Since

Dec. 15, 2014

Availability

Academic Institutions and Nonprofits only

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pBECKP-km

Plasmid

#117235

Purpose

A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae

Depositor

Quanjiang Ji

Article

Wang et al Appl Environ Microbiol. 2018 Sep 14. pii

Insert

ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Available Since

Nov. 7, 2018

Availability

Academic Institutions and Nonprofits only

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pCasTet-λ

Plasmid

#128318

Purpose

Constitutive expression of cas9 and anhydrotetracycline/tetracycline inducible expression of lamda RED. Useful variant of Plasmid #62225 when arabinose induction is not possible.

Depositor

Christopher Johnston

Article

Johnston et al Proc Natl Acad Sci U S A. 2019 May 16. p

Insert

TetR and pTetR/TetO

Use

CRISPR and Synthetic Biology

Expression

Bacterial

Mutation

Constitutive expression of cas9 and anhydro-tetra…

Promoter

tet promoter

Available Since

Aug. 22, 2019

Availability

Academic Institutions and Nonprofits only

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pU6-Cj-sgRNA

Plasmid

#89753

Purpose

U6 promoter driven expression of Cj-SgRNA cloned with BsmbI

Depositor

Seokjoong Kim

Article

Kim et al Nat Commun. 2017 Feb 21;8:14500. doi: 10

Insert

Cj-SgRNA

Use

Sgrna expression under u6 promoter

Promoter

U6

Available Since

May 25, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE Cacnb3-GFP KI

Plasmid

#139662

Purpose

Endogenous tagging of CaVβ3: C-terminal (amino acid position: W479)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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lenti F9-TetR-EGFP-IRES-PuroR

Plasmid

#117049

Purpose

lentiviral vector for expression of TetR-EGFP, for visualization of TetO repeats

Depositor

Huimin Zhao

Article

Tasan et al Nucleic Acids Res. 2018 Jun 15. pii: 503

Insert

F9-TetR-EGFP

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

F9 promoter

Available Since

March 7, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-sgRNA(lib)-puro

Plasmid

#119976

Purpose

Lenti sgRNA cloning backbone with CMV-puro resistance marker. Contains BsmBI sites for insertion of spacer sequences.

Depositor

Wensheng Wei

Article

Liu et al Nat Biotechnol. 2018 Nov 5. pii: nbt.428

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

March 11, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #3

Plasmid

#139659

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: A20)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

May 15, 2020

Availability

Academic Institutions and Nonprofits only

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pLV-U6-gRNA-UbC-eGFP-P2A-Bsr

Plasmid

#83925

Purpose

Lentiviral SpCas9-gRNA expression vector with eGFP-P2A-BlastR

Depositor

Charles Gersbach

Article

Klann et al Nat Biotechnol. 2017 Apr 3. doi: 10.1038

Inserts

eGFP
Bsr

Use

CRISPR and Lentiviral

Tags

P2A

Promoter

hUbC

Available Since

April 6, 2017

Availability

Academic Institutions and Nonprofits only

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pORANGE GFP-Grin1 KI #2

Plasmid

#139658

Purpose

Endogenous tagging of GluN1: N-terminal (amino acid position: D23)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

April 17, 2020

Availability

Academic Institutions and Nonprofits only

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pEJS469-pLK.O1-SpySgRNA/DTS13-Telomere

Plasmid

#85715

Purpose

Telomere-targeting Spy sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pBluescriptSKII+ U6-sgRNA(F+E) ACTB

Plasmid

#74705

Purpose

Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified scaffold (Chen et al. Cell 2013) and a spacer targeting the 3'UTR of ACTB mRNA

Depositor

Eugene Yeo

Article

Nelles et al Cell. 2016 Mar 16. pii: S0092-8674(16)30

Insert

U6 promoter driving sgRNA targeting the 3'UTR of ACTB mRNA

Use

CRISPR

Expression

Mammalian

Available Since

April 27, 2016

Availability

Academic Institutions and Nonprofits only

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pTPH413

Plasmid

#185728

Purpose

Bacterial PAM profiling of adenine base editor variants

Depositor

David Liu

Article

Huang et al Nat Biotechnol. 2022 Sep 8. pii: 10.1038

Inserts

ecTadA(8e)-neNme2-C
Nme2Cas9 sgRNA

Use

CRISPR

Expression

Bacterial

Mutation

Nme2Cas9 P6S/D16A/E33G/K104T/D152A/F260L/A263T/A3…

Available Since

Sept. 16, 2022

Availability

Academic Institutions and Nonprofits only

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AAV CMV-driven AcrIIC3-scaffold (2xBsmBI sites)

Plasmid

#120301

Purpose

AAV vector for expression of AcrIIC3 (no miR binding sites, control vector)

Depositor

Dominik Niopek

Article

Hoffmann et al Nucleic Acids Res. 2019 Apr 15. pii: 545

Insert

AcrIIC3

Use

AAV and CRISPR

Expression

Mammalian

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pORANGE Rims1-GFP KI

Plasmid

#131494

Purpose

Endogenous tagging of RIM1: C-terminal (amino acid position: S1615)

Depositor

Harold MacGillavry

Article

Willems et al PLoS Biol. 2020 Apr 10;18(4):e3000665. d

Insert

gRNA and GFP donor (Rims1 Rat)

Expression

Mammalian

Promoter

U6 and Cbh

Available Since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-NIrD

Plasmid

#65010

Purpose

NS3/4A Inducible rtTA-mediated Dual-reporter system for Hepatitis C Virus (HCV) infection

Depositor

Wensheng Wei

Article

Ren et al Sci Rep. 2015 Mar 9;5:8865. doi: 10.1038

Inserts

rtTA-MAVS(C)
2A-linked delta-TK

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

Tight-TRE

Available Since

Dec. 15, 2015

Availability

Academic Institutions and Nonprofits only
pEJS468-pLK.O1-NmeSgRNA/DTS13-Telomere

Plasmid

#85714

Purpose

Telomere-targeting Nme sgRNA under U6 promoter

Depositor

Erik Sontheimer

Article

Pawluk et al Cell. 2016 Dec 15;167(7):1829-1838.e9. d

Insert

telomere sgRNA

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

Jan. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pVAX_CBI_AcrIIA4

Plasmid

#136662

Purpose

Expresses human codon-optimized AcrIIA4 in mammalian cells. ORF flanked by NotI and XhoI restriction sites

Depositor

Yannick Doyon

Article

Hynes et al Nat Commun. 2018 Jul 25;9(1):2919. doi:

Insert

AcrIIA4

Use

Synthetic Biology

Tags

No tag, No NLS

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 28, 2020

Availability

Academic Institutions and Nonprofits only

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