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GFP-RAB10 T23N

Plasmid

#130885

Purpose

Expresses human RAB10 T23N mutant with N-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

RAB10 (RAB10 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Mutation

T23N

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLX317-CELF4

Plasmid

#115445

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

CELF4 (CELF4 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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DSPQ-HTT-TALEN2 BSD

Plasmid

#92246

Purpose

TALEN targeting downstream of HTT gene (ELD) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN1 ZEO. TALEN: NN, HD, NN, NN, HD, NG, NN, NI, NN, NN, HD, NI, NN, HD, NI, NN

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CAG

Available Since

Feb. 26, 2019

Availability

Academic Institutions and Nonprofits only

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DSPQ-HTT-TALEN1 ZEO

Plasmid

#92245

Purpose

TALEN targeting downstream of HTT gene (KKR) with Blasticidin selection, forms obligate heterodimer with DSPQ-HTT-TALEN2 BSD. TALEN: HD, NI, NN, HD, NG, NG, HD, HD, NG, HD, NI, NN, HD, HD, NN, HD

Depositor

Mahmoud Pouladi

Article

Ooi et al Cell Rep. 2019 Feb 26;26(9):2494-2508.e7

Insert

TALEN

Use

TALEN

Promoter

CAG

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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MYH11-NanoLuc-2A-tdTomato

Plasmid

#132569

Purpose

Donor vector to knockin NanoLuc and tdTomado (separated by 2A) into MYH11 allele

Depositor

James Thomson

Article

Zhang et al Stem Cell Reports. 2019 Jun 11;12(6):126

Insert

Human MYH11 knockin homology arms (MYH11 Human)

Expression

Mammalian

Available Since

Jan. 14, 2020

Availability

Academic Institutions and Nonprofits only

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pnEA-vH_His-TEV-SEPT2-msfGFP

Plasmid

#174492

Purpose

bacterial expression of human SEPT2 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Iv et al J Cell Sci. 2021 Jul 8. pii: 270842. doi

Insert

SEPTIN2 (SEPTIN2 Human)

Tags

His6-TEV and msfGFP

Expression

Bacterial

Available Since

Sept. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pBABE-hygro-2xMyc-Rac1-WT

Plasmid

#128580

Purpose

For mammalian cell expression of WT murine RAC1 carrying 2x Myc epitope tag sequences at the N-terminus.

Depositor

Julian Downward

Article

Lionarons et al Cancer Cell. 2019 Jul 8;36(1):68-83.e9.

Insert

Rac1 (Rac1 Mouse)

Use

Retroviral

Tags

2xMyc epitope

Expression

Mammalian

Available Since

Aug. 12, 2019

Availability

Academic Institutions and Nonprofits only

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FUW-tetO-loxP-hNANOG

Plasmid

#60849

Purpose

Lentiviral DOX-inducible expression vector for human NANOG

Depositor

Rudolf Jaenisch

Article

Theunissen et al Cell Stem Cell. 2014 Oct 2;15(4):471-87.

Insert

hNANOG (NANOG Human)

Use

Lentiviral

Available Since

Oct. 24, 2014

Availability

Academic Institutions and Nonprofits only

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AAVS1-A2UCOE-EF1a-hCD46-P2A-HLA-G SCT-T2A-hCD47-E2A-hCD59-F2A-neo-AAVS1

Plasmid

#205443

Purpose

human AAVS1-targeting plasmid for expression of iCasp9, HLA-E SCT, human and CD55

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

hCD46-HLA-G SCT-hCD47-hCD59

Expression

Mammalian

Promoter

EF1a

Available Since

Sept. 27, 2023

Availability

Academic Institutions and Nonprofits only

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pGL3-noSV40-humanCOL2A1enhancer

Plasmid

#173961

Purpose

Firefly luciferase enhancer reporter plasmid.

Depositor

Joanna Wysocka

Article

Long et al Cell Stem Cell. 2020 Nov 5;27(5):765-783

Insert

Enhancer region from COL2A1 gene

Use

Luciferase

Mutation

None

Promoter

SV40 promoter

Available Since

Sept. 3, 2021

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-NTRK1(FL)-FLAG

Plasmid

#157620

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

NTRK1 (NTRK1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 13, 2023

Availability

Academic Institutions and Nonprofits only

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C1ORF43-GFP

Plasmid

#130879

Purpose

Expresses human C1ORF43 with C-terminal GFP tag

Depositor

Michael Bassik

Article

Jeng et al Cell Host Microbe. 2019 Oct 9;26(4):551-

Insert

C1ORF43 (C1orf43 Human)

Use

Lentiviral

Tags

GFP

Expression

Mammalian

Available Since

Sept. 18, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4405

Plasmid

#115466

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4405 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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IL2R/hE-cadherin-cytotail

Plasmid

#45773

Depositor

Barry Gumbiner

Article

Gottardi et al J Cell Biol. 2001 May 28;153(5):1049-60.

Insert

IL2R/hE-cadherin-cytotail (IL2RA, CDH1 Human)

Expression

Mammalian

Mutation

Interleukin-2 receptor α subunit extracellular an…

Promoter

CMV

Available Since

June 28, 2013

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL1(FL)-FLAG

Plasmid

#157625

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL1 (KIR2DL1 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pTT3-SP-6xHis-KIR2DL4(FL)-FLAG

Plasmid

#157624

Purpose

Mammalian cell surface expression of His- and FLAG-tagged full-length protein for binding and signaling assays

Depositor

Chris Garcia

Article

Wojtowicz et al Cell. 2020 Aug 20;182(4):1027-1043.e17.

Insert

KIR2DL4 (KIR2DL4 Human)

Tags

6xHis and FLAG

Expression

Mammalian

Mutation

Promoter

CMV

Available Since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+ Flag-RAP2A

Plasmid

#128099

Purpose

Expresses RAP2A in mammalian cells in a G418-selectable plasmid

Depositor

Chi Van Dang

Article

Walton et al Cell. 2018 Jun 28;174(1):72-87.e32. doi:

Insert

RAP2A (RAP2A Human)

Tags

Flag

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLKO.1-shCELF4-4404

Plasmid

#115465

Purpose

Constitutive lentiviral expression

Depositor

William Hahn

Article

Li et al Elife. 2018 Jul 30;7. pii: 37184. doi: 1

Insert

shCELF4-4404 (CELF4 Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Available Since

Sept. 17, 2018

Availability

Academic Institutions and Nonprofits only

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Cav2.1 HA pcDNA3

Plasmid

#206069

Purpose

expression of rat Cav2.1 calcium channel with a E1686R mutation to enhance expression and an exofacial double HA tag in domain II

Depositor

Annette Dolphin

Article

Meyer et al Cell Rep. 2019 Oct 1;29(1):22-33.e5. doi

Insert

cacna1a (Cacna1a Rat)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 14, 2023

Availability

Academic Institutions and Nonprofits only

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BII-BCA-Prom-ASCL2

Plasmid

#133394

Purpose

Piggybac vector for heterologous promoter reporter assay of human ASCL2 promoter

Depositor

Blair Madison

Article

Strubberg et al Stem Cell Reports. 2018 Aug 14;11(2):410

Insert

destabilized tdTomato-NLS and destabilized NLS-GFP

Expression

Mammalian

Mutation

N/A

Promoter

CMV enhancer and hEf1a (CpG free) drives expressi…

Available Since

Jan. 10, 2020

Availability

Academic Institutions and Nonprofits only

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