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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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Showing: 9961 - 9990 of 10834 results

  5. pDNA2.0 6H-TEV-KRAS G12V

    Plasmid
    #111850
    Purpose
    Expresses activated human KRAS G12V (amino acids 1 - 169) in E. coli with amino terminal 6xHIS tag that can be removed with TEV protease.
    Depositor
    Kenneth Westover
    Article
    Hunter et al Mol Cancer Res. 2015 Sep;13(9):1325-35.
    Insert
    KRAS G12V (KRAS Human)
    Tags
    6xHis-tag and TEV protease cleavage sequence
    Expression
    Bacterial
    Mutation
    Changed Glycine 12 to Valine
    Promoter
    T5
    Available Since
    June 20, 2018
    Availability
    Academic Institutions and Nonprofits only
    111850-plasmid-map-sequence-id-213464

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  17. pNQL001-WAPL-AID[71-114]-eGFP-FRT-Neo/Kan-FRT targeting construct

    Plasmid
    #175549
    Purpose
    Targeting vector to introduce an AID-eGFP cassette at the mouse WAPL locus using Neomycin/Kanamycin selection. Auxin-inducible degron system.
    Depositor
    Elzo de Wit
    Article
    Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:
    Insert
    WAPL (Wapl Mouse)
    Use
    Mouse Targeting
    Tags
    AID[71-114]-eGFP
    Expression
    Mammalian
    Mutation
    none
    Available Since
    Nov. 5, 2021
    Availability
    Academic Institutions and Nonprofits only
    175549-plasmid-map-sequence-id-345088

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  22. mEos3.2GG

    Plasmid
    #203756
    Purpose
    Encodes a modified version of the membrane anchor of KRas, including the last 16 residues of the C terminus, with mEos3.2 fused to the N terminus. The base pairs encoding the CAAX box for KRas were replaced by the CAAX box from rap1B, resulting in a geranylgeranylation instead of farnesylation. Used as a fluorescent plasma membrane probe.
    Depositor
    Sarah Veatch
    Article
    Shelby et al Nat Chem Biol. 2023 Jun;19(6):750-758. d
    Insert
    KRas c-term (KRAS Human)
    Tags
    mEos3.2
    Expression
    Mammalian
    Available Since
    Aug. 16, 2023
    Availability
    Academic Institutions and Nonprofits only
    203756-plasmid-map-sequence-id-404785

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Showing: 9961 - 9990 of 10834 results