We narrowed to 18,937 results for: Tec

1. pX459-sg-mGreenLantern332

   Plasmid

   #179914

   Purpose

   All in one Cas9 plasmid with puromycin resistance and a single guide RNA targeting mGreenLantern fluorescent protein site 332.

   Depositor

   Indeever Madireddy, Johan Sosa

   Article

   Madireddy et al MicroPubl Biol. 2022 Jun 16;2022. doi: 1

   Insert

   mGreenLantern sgRNA
   

   Use

   CRISPR

   Tags

   2A-Puro and 3XFLAG

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   April 4, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. 1137B=Hsp70Bb-Cas9

   Plasmid

   #153284

   Purpose

   The attB plasmid harboring the Hsp70Bb-Cas9-T2A-eGFP-p10 and a mini-white marker.

   Depositor

   Omar Akbari

   Article

   Kandul et al CRISPR J. 2021 Dec;4(6):822-835. doi: 10

   Insert

   pHsp70Bb-SpCas9-T2A-eGFP-p10 (cas9 Synthetic)
   

   Use

   CRISPR

   Tags

   eGFP

   Expression

   Insect

   Promoter

   Dmel Hsp70Bb

   Available Since

   March 30, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUC19-SpRYgest_targetplasmid (KAC833)

   Plasmid

   #181748

   Purpose

   pUC19 derivative plasmid used as a substrate for SpRYgest, restriction enzyme, and TtAgo digests. Encodes an SpCas9 EMX1-site1-NGG-PAM target site between NheI and HindIII restriction sites.

   Depositor

   Benjamin Kleinstiver

   Article

   Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

   Type

   Empty backbone

   Use

   Substrate plasmid

   Available Since

   Feb. 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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4. pG.Lantern-INS-SM50-GFP

   Plasmid

   #135594

   Purpose

   Propogation of Insulator-SM50 Promoter-GFP DNA for microinjection to visualize Insulator-GFP promoter activity in S. purpuratus

   Depositor

   Koji Akasaka, Mamiko Yajima

   Article

   Yajima et al Dev Genes Evol. 2007 Apr;217(4):331-6. d

   Insert

   INS-SM50 promoter (SM50 )
   

   Use

   In vitro transcription (mrna synthesis)

   Tags

   Green Lantern GFP

   Promoter

   CMV

   Available Since

   Jan. 7, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBS-tRNA-Gly

   Plasmid

   #174317

   Purpose

   For in vitro transcription of mouse tRNAs

   Depositor

   Bruce Beutler

   Article

   Yue et al Science. 2021 May 14;372(6543). pii: 372

   Insert

   tRNA-gly
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   Nov. 23, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. PdnaK-IR3 GFP pZa

   Plasmid

   #170084

   Purpose

   GFP expression under the control of E. coli dnaK promoter engineered with IR3 HAIR motif from M. tuberculosis

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PdnaK-IR3

   Available Since

   Sept. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. PdnaK-IR2 GFP pZa

   Plasmid

   #170081

   Purpose

   GFP expression under the control of E. coli dnaK promoter engineered with IR2 HAIR motif from M. tuberculosis

   Depositor

   Urartu Seker

   Article

   Saltepe et al ACS Synth Biol. 2019 Oct 18;8(10):2404-2

   Insert

   Green fluorescent protein
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   PdnaK-IR2

   Available Since

   Sept. 10, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET28a(+)-HisTim12_CtoA

   Plasmid

   #170281

   Purpose

   Expresses yeast Tim12 (the latter with cleavable N-ter His-tag), codon-optimized for E. coli production

   Depositor

   Paul Schanda

   Article

   Weinhaupl et al Structure. 2021 May 3. pii: S0969-2126(2

   Insert

   yeast Tim12 (TIM12 Budding Yeast)
   

   Tags

   TEV-cleavable N-terminal His tag (on backbone)

   Expression

   Bacterial

   Mutation

   mutated two non-conserved Cys to Ala

   Available Since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. KG#920

   Plasmid

   #110937

   Purpose

   Expresses INS-22-Emerald in the cholinergic motor neuron DA9 for imaging Dense Core Vesicles in a single neuron in a living animal

   Depositor

   Kenneth Miller

   Article

   Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

   Inserts

   mig-13 promoter
   INS-22-Emerald
   unc-54 3' control region with 1 artificial intron just upstream and 1 artificial intron in control region
   

   Expression

   Bacterial

   Available Since

   March 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. pUE1-TSP-ORF68 R14A/K310A

    Plasmid

    #162655

    Purpose

    Expresses ORF68 R14A/K310A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids R14 and K310 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLJM1-zeo-2xStrep-ORF68 C52A

    Plasmid

    #162660

    Purpose

    Lentiviral vector for stable expression of N-terminal 2xStrep-tagged ORF68 C52A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Use

    Lentiviral

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acid C52 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO-2xStrep-ORF68 K174A/R179A/K182A

    Plasmid

    #162639

    Purpose

    Expresses N-terminal 2x-Strep-tagged ORF68 K174A/R179A/K182A in mammalian cells

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II

    Expression

    Mammalian

    Mutation

    Amino acids K174, R179, and K182 mutated to alani…

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pUE1-TSP-ORF68 K435A

    Plasmid

    #162651

    Purpose

    Expresses ORF68 K435A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acid K435 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pUE1-TSP-ORF68 K450A/R451A

    Plasmid

    #162653

    Purpose

    Expresses ORF68 K450A/R451A in mammalian cells with an N-terminal TwinStrep tag and HRV 3C protease cleavage site

    Depositor

    Britt Glaunsinger

    Article

    Didychuk et al Elife. 2021 Feb 8;10. pii: 62261. doi: 1

    Insert

    ORF68
    

    Tags

    N-terminal 2xStrep-Tag II and HRV 3C site

    Expression

    Mammalian

    Mutation

    Amino acids K450 and R451 mutated to alanine

    Promoter

    CMV

    Available Since

    Feb. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCTCON2-BXG-altTAG

    Plasmid

    #158132

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the first serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCTCON2-BXG-altTAG2

    Plasmid

    #158134

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the second serine residu…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCTCON2-BXG-altTAG3

    Plasmid

    #158136

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third alanine residu…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCTCON2-BXG-altTAG4

    Plasmid

    #158137

    Purpose

    Dual fluorescent protein reporter (BFP-GFP) for ncAA incorporation (TAG construct)

    Depositor

    James Van Deventer

    Article

    Potts et al Mol Syst Des Eng. 2020 Feb 1;5(2):573-58

    Insert

    BFP-GFP fragment
    

    Expression

    Yeast

    Mutation

    Cloned in a TAG codon at the third serine residue…

    Promoter

    GAL1-10

    Available Since

    Jan. 12, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pSWD95Y14D

    Plasmid

    #159098

    Purpose

    CckA FRET Sensor with cyclic-di-GMP point mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691(Y514D), Linker SNVTRHRSAT, mRuby3
    

    Expression

    Bacterial

    Mutation

    Mutated Y514 to D514

    Promoter

    Xylose

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSWD95D293A

    Plasmid

    #159099

    Purpose

    CckA FRET Sensor with DITE motif mutant

    Depositor

    Seth Childers

    Article

    Duvall et al ACS Sens. 2020 Jun 26;5(6):1589-1596. do

    Insert

    CckA 1-182, Linker SNVTRHRSATE, mClover3, CckA 183-691 (D293A), Linker SNVTRHRSAT, mRuby3
    

    Expression

    Bacterial

    Mutation

    CckA D293 mutated to A293 and V199I on mRuby3 (P…

    Promoter

    Xylose

    Available Since

    Nov. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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