We narrowed to 9,742 results for: Tec;

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pcDNA216-CoV2-RBD-beta variant

Plasmid

#194598

Purpose

Mammalian expression of RBD domain from SARS-CoV-2 beta (B.1.351)

Depositor

Ben Murrell

Article

Sheward et al Cell Rep Med. 2021 Nov 16;2(11):100450.

Insert

CoV2-RBD-beta variant (S Severe acute respiratory syndrome coronavirus 2)

Expression

Mammalian

Available Since

Jan. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pTS1051-Tier1-PhCMV-4xCox8

Plasmid

#169490

Purpose

Tier-1 vector encoding PhCMV-driven four-fold repeat of a mitochondrial matrix localization signal derived from mitochondrial matrix protein Cox8 (PhCMV-4xCox8-pA)

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PCMV-driven mitochondrial localization signal from cytochrome c oxidase subunit 8

Expression

Mammalian

Promoter

PhCMV

Available Since

June 14, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 R10A R15A-msfGFP

Plasmid

#180336

Purpose

mammalian expression of human SEPT9_i1 R10A R15A fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R10A R15A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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pCMV_SEPT9_i1 NCmut1-msfGFP

Plasmid

#180329

Purpose

mammalian expression of human SEPT9_i1 NCmut1 fused to monomeric superfolder GFP

Depositor

Manos Mavrakis

Article

Kuzmic et al J Cell Sci. 2021 Dec 2. pii: 273604. doi

Insert

SEPTIN9_v1 (SEPTIN9 Human)

Tags

msfGFP

Expression

Mammalian

Mutation

SEPT9_i1 R289A R290A K291A K294A

Promoter

CMV

Available Since

March 29, 2022

Availability

Academic Institutions and Nonprofits only

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mILK-L207W-EFGP

Plasmid

#176900

Purpose

mouse ILK with L207W point mutation cloned into pEGFP-N1

Depositor

Sara Wickström

Article

Martin et al Proc Natl Acad Sci U S A. 2022 Mar 15;11

Insert

Integrin-linked kinase (Ilk Mouse)

Tags

EGFP

Expression

Mammalian

Mutation

Leucine 207 to Tryptophane

Promoter

CNV

Available Since

March 22, 2022

Availability

Academic Institutions and Nonprofits only

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TPC2(E643A)-mCherry

Plasmid

#135187

Purpose

Mutant TPC2 with reduced Ca2+ selectivity, tagged on C terminus with mCherry.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TPC2 (Tpcn2 Mouse)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Mutation

Mutation of glutamate 643 to alanine

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S197A

Plasmid

#131522

Purpose

Expresses MKP-3 with S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Mutation

changed Serine 197 to Alanine

Promoter

CMV

Available Since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-bSV1_1-1009

Plasmid

#129471

Purpose

Mammalian expression of the N-terminus + central domain of bovine SV1

Depositor

Elizabeth Luna

Article

Wulfkuhle et al J Cell Sci. 1999 Jul:112 (Pt 13):2125-36

Insert

Supervillin (SVIL Bovine)

Expression

Mammalian

Mutation

contains amino acids 1-1009

Promoter

CMV

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pJEP320-pAAV-U6SaCas9gRNA(CREB3)_EFS-GFP-KASH-pA

Plasmid

#113697

Purpose

U6 driven SaCas9 gRNA expression cassette with a gRNA targeting CREB, followed by an EFS driven GFP-KASH in a separate reading frame.

Depositor

Jonathan Ploski

Article

Kumar et al Front Mol Neurosci. 2018 Nov 13;11:413.

Inserts

SaCas9 gRNA Cassete
GFP

Use

AAV and CRISPR

Tags

KASH

Available Since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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HA-BAP1-N102S-GFP

Plasmid

#78915

Purpose

Mammalian expression of BAP1 with N102S and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

N102S

Promoter

CMV

Available Since

July 20, 2016

Availability

Academic Institutions and Nonprofits only
HA-BAP1-C91G-GFP

Plasmid

#78916

Purpose

Mammalian expression of BAP1 with C91G and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Cancer Res. 2014 Aug 15;74(16):4282-94.

Insert

BAP1 (BAP1 Human)

Tags

EGFP and HA

Expression

Mammalian

Mutation

C91G

Promoter

CMV

Available Since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
mini-YFP-hDF-C2F-TM

Plasmid

#72600

Purpose

mini human dysferlin (from domain F to transmembrane ) tagged with YFP

Depositor

Renzhi Han

Article

Cheng et al Nat Med. 2014 Oct;20(10):1187-92. doi: 1

Insert

dysferlin (DYSF Human)

Tags

EYFP

Expression

Mammalian

Mutation

from domain F to transmembrane domain

Promoter

CMV

Available Since

April 15, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-C1-MKLP1 S710A

Plasmid

#70149

Purpose

mammalian expression of MKLP1 with an eGFP fusion and an S710A mutation

Depositor

Masanori Mishima

Article

Douglas et al Curr Biol. 2010 May 25;20(10):927-33. do

Insert

MKLP1 (KIF23 Human)

Tags

eGFP

Expression

Mammalian

Mutation

S710A

Promoter

CMV

Available Since

Nov. 4, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) Laccase2 Sense

Plasmid

#69892

Purpose

Expresses the Drosophila laccase2 exon 2 circular RNA

Depositor

Jeremy Wilusz

Article

Kramer et al Genes Dev. 2015 Oct 8.

Insert

Laccase2 (stw Fly)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pDEST47-ARL3

Plasmid

#67460

Purpose

Mammalian expression of hArl3 with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARL4C

Plasmid

#67465

Purpose

Mammalian expression of hArl4c with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL7 (ARL4C Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST47-ARFRP1

Plasmid

#67471

Purpose

Mammalian expression of hArfrp with stop codon (native expression)

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARFRP1 (ARFRP1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pET-DEST42-ARL4-V5-His6

Plasmid

#67419

Purpose

Expression of hArl4 with C-term V5 and His6 tag in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL4 (ARL4A Human)

Tags

His and V5

Expression

Bacterial

Mutation

bp 105 A to T; silent mutation

Promoter

T7/lacO

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-F164L

Plasmid

#64681

Purpose

expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at F164L

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-H376Y

Plasmid

#64682

Purpose

expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Tags

GFP

Expression

Mammalian

Mutation

mutation at H376Y

Promoter

CMV

Available Since

May 21, 2015

Availability

Academic Institutions and Nonprofits only