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pRSETa mEos2

Plasmid

#20341

Purpose

bacterial expression of an EosFP variant, mEos2 (a photoconvertible fluorescent protein)

Depositor

Loren Looger

Article

McKinney et al Nat Methods. 2009 Feb . 6(2):131-3.

Insert

mEos-2

Tags

His

Expression

Bacterial

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only

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pCre

Plasmid

#236212

Purpose

Arabinose-inducible Cre recombinase

Depositor

Matthew Waldor

Article

Basta et al Nat Microbiol. 2025 Mar 27. doi: 10.1038

Insert

Cre recombinase

Expression

Bacterial

Promoter

araBAD promoter

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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MAVS-WT

Plasmid

#52135

Purpose

Retroviral vector for expression of human MAVS in mammalian cells

Depositor

Jonathan Kagan

Article

Dixit et al Cell. 2010 May 14;141(4):668-81. Epub 20

Insert

MAVS (MAVS Human)

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2014

Availability

Academic Institutions and Nonprofits only

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AAV-hSyn-RiboL1-jGCaMP8s

Plasmid

#169247

Purpose

AAV transfer plasmid for neuronal expression of soma-targeted (RL-10, ribosomal tag) jGCaMP8s, with a flexible GS-linker sequence attaching the ribosomal tag to the jGCaMP8s protein.

Depositor

Marianne Fyhn

Article

Grodem et al Nat Commun. 2023 Feb 4;14(1):608. doi: 1

Insert

RiboL1-jGCaMP8s

Use

AAV

Tags

6xHis

Expression

Mammalian

Promoter

hSyn

Available Since

Oct. 7, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-Sport6-CD63-pHluorin

Plasmid

#130901

Purpose

Expression of CD63-pHluorin for visualization of multivesicular body-plasma membrane fusion.

Depositor

DM Pegtel

Article

Verweij et al J Cell Biol. 2018 Mar 5;217(3):1129-1142

Insert

CD63-pHluorin (CD63 Aequorea victoria, Human)

Tags

pHluorin

Expression

Mammalian

Mutation

pHluorin inserted between Gln-36 and Leu-37 in ex…

Promoter

CMV

Available Since

Oct. 10, 2019

Availability

Academic Institutions and Nonprofits only

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pLEX-FHH-Empty Vector-IRES-Puro

Plasmid

#120568

Purpose

For cloning genes with an N-terminal TAP tag [Flag-HA-6xHIS]

Depositor

Paul Khavari

Article

Kovalski et al Mol Cell. 2019 Jan 4. pii: S1097-2765(18

Type

Empty backbone

Use

Lentiviral

Tags

Flag-HA-6xHis

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 30, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti-puro-ARID1A

Plasmid

#39478

Purpose

Tetracycline-inducible lentiviral expression of human ARID1A; 3rd generation lentiviral vector

Depositor

Ie-Ming Shih

Article

Guan et al Cancer Res. 2011 Nov 1;71(21):6718-27. E

Insert

ARID1A (ARID1A Human)

Use

Lentiviral

Tags

His and V5

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 13, 2012

Availability

Academic Institutions and Nonprofits only

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pCW57.1 Lysosomal-METRIQ

Plasmid

#135401

Purpose

Expresses lysosomal-METRIQ (DNAse II-sfGFP together with cytosolic RFP) to measure lysosomal activity

Depositor

Eisuke Itakura

Article

Ishii et al Sci Rep. 2019 Aug 12;9(1):11635. doi: 10

Insert

DNAse II alpha (DNASE2 Human)

Use

Lentiviral

Tags

T2A, mCherry, and sfGFP

Expression

Mammalian

Mutation

Single-nucleotide polymorphism C432T

Available Since

Feb. 26, 2020

Availability

Academic Institutions and Nonprofits only

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pLL3.7m-rsGreenF-E-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-Skylan62A-2A-YFP-SLBP

Plasmid

#205762

Purpose

Single-color FUCCI4 senor for transitions of all four cell cylce phases

Depositor

Edward Boyden

Article

Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

Insert

rsGFE-Geminin(1-110)-2A-Dronpa-Cdt-IRES-H1-SK62A-2A-YFP-SLBP

Use

Lentiviral

Expression

Mammalian

Mutation

wt

Available Since

Oct. 18, 2023

Availability

Academic Institutions and Nonprofits only

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Akaluc-N1

Plasmid

#120371

Purpose

Akaluc luciferase mammalian expression in -N1 backbone

Depositor

Jordan Green

Article

Karlsson et al Adv Funct Mater. 2021 Apr 22;31(17). doi

Insert

Akaluc (human optimized)

Expression

Mammalian

Mutation

Human codon optimized

Promoter

CMV

Available Since

Feb. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMK232 (CMV-OsTIR1-PURO)

Plasmid

#72834

Purpose

Expresses OsTIR1 under the control of the CMV promoter

Depositor

Masato Kanemaki

Article

Natsume et al Cell Rep. 2016 Apr 5;15(1):210-8. doi: 1

Insert

AAVS1-CMV-OsTIR1-PURO-AAVS1

Expression

Mammalian

Available Since

Feb. 5, 2016

Availability

Academic Institutions and Nonprofits only

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pG2K

Plasmid

#71742

Purpose

Modular shuttle vector for Geobacillus and E. coli

Depositor

Tom Ellis

Article

Reeve et al ACS Synth Biol. 2016 Jul 1.

Type

Empty backbone

Use

Synthetic Biology; Shuttle vector

Expression

Bacterial

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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pLV-UBC mTagBFP2-P2A-3xFLAG-hEEA1

Plasmid

#214995

Purpose

Express the human EndoTag (3xFLAG-EEA1) for EndoIPs. mTagBFP2 to sort for even expression levels.

Depositor

Wade Harper

Article

Hollingsworth et al bioRxiv 2024.04.19.590338

Insert

EEA1 (EEA1 Human)

Use

Lentiviral

Tags

3xFlag

Expression

Mammalian

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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mCherry-Rab11a-7

Plasmid

#55124

Purpose

Localization: Ras GTPase, Excitation: 587, Emission: 610

Depositor

Michael Davidson

Insert

Rab11a (RAB11A Human)

Tags

mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 29, 2014

Availability

Academic Institutions and Nonprofits only

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tetO-GFPnls-mPGK-rtTA-IRES-Puro

Plasmid

#69545

Purpose

Tet-On system expressing GFP tagged with 2x nuclear localization signals.

Depositor

Charles Gersbach

Insert

eGFP

Use

Lentiviral

Tags

2x nuclear localization sequence and T2A "sk…

Available Since

April 12, 2019

Availability

Academic Institutions and Nonprofits only

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AAVS1-Puro CAG PSMA nanobody T-CAR

Plasmid

#171962

Purpose

Donor plasmid for PSMA nanobody T-CAR expression in human cells

Depositor

Xiaoping Bao

Article

Harris et al J Immunol Regen Med. 2023 May;20:100074.

Insert

Synthetic anti-PSMA nanobody CAR

Use

CRISPR, Synthetic Biology, and TALEN

Expression

Mammalian

Promoter

CAG

Available Since

May 5, 2023

Availability

Academic Institutions and Nonprofits only

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OMM-short-RspA-CFAST

Plasmid

#233594

Purpose

Expression of RspA-CFAST on the outer mitochondrial membrane

Depositor

Riccardo Filadi

Article

Garcia Casas et al Nat Commun. 2024 Nov 12;15(1):9775. doi:

Insert

TOM70 fragment-RspA-CFAST (TOMM70 Human, RspA-CFAST from Rheinheimera sp A13L and TOM70 fragment from Homo sapiens)

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3-Casp3-myc

Plasmid

#11813

Depositor

Guy Salvesen

Article

Stennicke et al J Biol Chem. 1997 Oct 10. 272(41):25719-

Insert

Caspase 3 (CASP3 Human)

Tags

Myc

Expression

Mammalian

Promoter

CMV

Available Since

May 25, 2006

Availability

Academic Institutions and Nonprofits only

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pEGFP-N1/PD-L1

Plasmid

#121478

Purpose

expression of human PD-L1 in mammalian cells

Depositor

Mien-Chie Hung

Article

Li et al Nat Commun. 2016 Aug 30;7:12632. doi: 10

Insert

CD274 (CD274 Human)

Tags

EGFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 2, 2021

Availability

Academic Institutions and Nonprofits only

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qTAG-C-dTAG-Zeo

Plasmid

#207738

Purpose

Empty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a dTAG-2A-Zeo cassette

Depositor

Laurence Pelletier

Article

Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318

Type

Empty backbone

Use

CRISPR; Donor cassette

Expression

Mammalian

Available Since

Nov. 15, 2023

Availability

Industry, Academic Institutions, and Nonprofits

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