19,658 results

1. pLKO.DEST.EGFP

   Plasmid

   #32684

   Purpose

   Lentiviral cDNA/shRNA gateway destination vectors

   Depositor

   Ming-Sound Tsao

   Article

   Radulovich et al BMC Biotechnol. 2011 Mar 22;11:24.

   Type

   Empty backbone

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 1, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pLVX-IRES-Hygro_GCaMP6s

   Plasmid

   #164591

   Purpose

   Stable integration and expression of GCaMP6s in mammalian cells

   Depositor

   Joseph Hyser

   Article

   Chang-Graham et al Sci Rep. 2019 Jul 25;9(1):10822. doi: 10

   Insert

   GCaMP6s
   

   Use

   Lentiviral

   Tags

   6xHis

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 25, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3.1(+)-HA-Flag-natT1R2

   Plasmid

   #113944

   Purpose

   mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin

   Depositor

   Erik Procko

   Article

   Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

   Insert

   T1R2 (TAS1R2 Human)
   

   Tags

   FLAG and Signal/leader sequence from influenza he…

   Expression

   Mammalian

   Mutation

   residues 22-839

   Available Since

   Feb. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. FokI-dCas9

   Plasmid

   #52970

   Purpose

   Expresses Fok1 nuclease domain fused to catalytically inactive Cas9 DNA-binding domain in mammalian cells

   Depositor

   David Liu

   Article

   Guilinger et al Nat Biotechnol. 2014 Apr 25. doi: 10.103

   Insert

   Fok1 fused to dCas9
   

   Use

   CRISPR

   Tags

   Fok1 nuclease (homodimeric wildtype) and NLS

   Expression

   Mammalian

   Mutation

   D10A and H840A in Cas9

   Promoter

   CMV

   Available Since

   May 21, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV-FLAG-hIKAROS-IRES-GFP

   Plasmid

   #74046

   Purpose

   retroviral plasmid for expression of FLAG-tagged human Ikaros corresponding to cDNA from XM_011515058.1

   Depositor

   Ria Baumgrass

   Article

   Gabriel et al J Biol Chem. 2016 Sep 16. pii: jbc.M116.

   Insert

   human Ikaros (IKZF1) (IKZF1 Human)
   

   Use

   Retroviral

   Tags

   FLAG

   Expression

   Mammalian

   Promoter

   pMSCV-LTRs

   Available Since

   June 8, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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6. HA-MAP1LC3B

   Plasmid

   #137757

   Purpose

   Expression of LC3B

   Depositor

   Carol Mercer

   Article

   Stefely et al Autophagy. 2020 Jan 23. doi: 10.1080/155

   Insert

   MAP1LC3B (MAP1LC3B Human)
   

   Tags

   HA and myc

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 26, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pAAV-HDR-mEGFP-camk2a

   Plasmid

   #104589

   Purpose

   AAV vector including gRNA expression cassette and mEGFP knock-in donor targeting mouse camk2a

   Depositor

   Ryohei Yasuda

   Article

   Nishiyama et al Neuron. 2017 Oct 18. pii: S0896-6273(17)

   Insert

   calcium/calmodulin-dependent protein kinase II alpha (Camk2a Mouse)
   

   Use

   AAV, CRISPR, and Mouse Targeting

   Promoter

   None

   Available Since

   Dec. 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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8. TLCV2-LoxP

   Plasmid

   #127098

   Purpose

   LentiCRISPR v2 was modified into an all-in-one dox inducible system.

   Depositor

   Adam Karpf

   Insert

   Cas9-2A-eGFP
   

   Use

   Lentiviral

   Promoter

   U6

   Available Since

   June 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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9. AAV-EFS-NLS-CasRx-U6-DR-sgRNA_LacZ-DR

   Plasmid

   #154003

   Purpose

   Vector expressed CasRx and sgRNA_LACZ for AAV package

   Depositor

   Hui Yang

   Article

   He et al Protein Cell. 2020 Mar 18. pii: 10.1007/

   Insert

   CasRx
   

   Use

   AAV and CRISPR

   Promoter

   EFS

   Available Since

   July 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. pENTR-AIF

    Plasmid

    #16182

    Depositor

    Huda Zoghbi

    Article

    Lim et al Cell. 2006 May 19. 125(4):801-14.

    Insert

    Apoptosis-inducing factor (AIFM1 Human)
    

    Expression

    Mammalian

    Available Since

    Jan. 18, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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11. epB_UbC_CymRV5-nls-GFP_DEx2

    Plasmid

    #119906

    Purpose

    ePiggyBac mammalian expression plasmid for CymR-GFP fusion

    Depositor

    Orion Weiner

    Article

    Alexander et al Elife. 2019 May 24;8. pii: 41769. doi: 1

    Insert

    CymRV5-nls-GFP
    

    Expression

    Mammalian

    Available Since

    June 6, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. RP4-8

    Plasmid

    #79813

    Purpose

    Modified broad host range plasmid RP4. Gentamicin resistant. Helper plasmid for conjugation

    Depositor

    Michael Hynes

    Article

    Quandt et al Plasmid. 2004 Jul;52(1):1-12.

    Insert

    Gentamicin resistance gene
    

    Use

    Helper bhr plasmid for mobilizing other plasmids …

    Promoter

    Own promoter

    Available Since

    Aug. 19, 2016

    Availability

    Academic Institutions and Nonprofits only

13. 1136- Desmoplakin-GFP

    Plasmid

    #32227

    Depositor

    Kathleen Green

    Article

    Godsel et al J Cell Biol. 2005 Dec 19;171(6):1045-59.

    Insert

    Desmoplakin (DSP Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 8, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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14. FLHKI-pGFPN3

    Plasmid

    #21917

    Depositor

    Hossein Ardehali

    Article

    Sun et al Mol Cell Biol. 2008 Feb . 28(3):1007-17.

    Insert

    Full length human HKI in pGFP-N3 (HK1 Human)
    

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    This is full length human HKI in pGFP-N3

    Available Since

    Sept. 17, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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15. pECE

    Plasmid

    #26453

    Depositor

    William Rutter

    Article

    Ellis et al Cell. 1986 Jun 6;45(5):721-32.

    Type

    Empty backbone

    Expression

    Mammalian

    Available Since

    Nov. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCIP-AMPKa1_KD

    Plasmid

    #79011

    Purpose

    For mammalian expression of a kinase dead (K47R) version of AMPK alpha1.

    Depositor

    Reuben Shaw

    Article

    Young et al Genes Dev. 2016 Mar 1;30(5):535-52. doi:

    Insert

    AMPK alpha 1 (PRKAA1 Human)
    

    Expression

    Mammalian

    Mutation

    K47R

    Promoter

    pCAGGs

    Available Since

    July 5, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-CMV-SauriCas9

    Plasmid

    #135964

    Purpose

    Expresses SauriCas9, and cloning backbone for sgRNA

    Depositor

    Yongming Wang

    Article

    Hu et al PLoS Biol. 2020 Mar 30;18(3):e3000686. d

    Type

    Empty backbone

    Use

    AAV

    Expression

    Mammalian

    Available Since

    April 9, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SGEP

    Plasmid

    #111170

    Purpose

    miR-E (miR-30 variant)-based RNAi

    Depositor

    Johannes Zuber

    Article

    Fellmann et al Cell Rep. 2013 Dec 26;5(6):1704-13. doi:

    Insert

    miR-E (miR-30 variant)
    

    Use

    Lentiviral

    Mutation

    WT

    Promoter

    SFFV

    Available Since

    May 31, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pJKR-H-tetR

    Plasmid

    #62561

    Purpose

    Tetracycline biosensor. High copy plasmid that expresses GFP proportional to tetracycline concentration.

    Depositor

    George Church

    Article

    Rogers et al Nucleic Acids Res. 2015 Jul 7. pii: gkv6

    Insert

    GFP
    

    Expression

    Bacterial

    Available Since

    Aug. 13, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCDNA3.1(+)-NEMOc

    Plasmid

    #189933

    Purpose

    Mammalian expression of calcium sensor with ultra-high dynamics and sensitivity

    Depositor

    Yubin Zhou

    Article

    Li et al Nat Methods. 2023 Jun;20(6):918-924. doi

    Insert

    NEMOc
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 9, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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