19,815 results

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803 pGEX2T PTEN

Plasmid

#10784

Depositor

William Sellers

Insert

PTEN (PTEN Human)

Tags

GST

Expression

Bacterial

Available Since

Nov. 14, 2005

Availability

Academic Institutions and Nonprofits only
pCS Omomyc

Plasmid

#113164

Purpose

Omomyc expression in mammalian cells

Depositor

Sergio Nasi

Article

Soucek et al Oncogene. 1998 Nov 12;17(19):2463-72. do

Insert

Omomyc (MYC Human)

Expression

Mammalian

Mutation

E57T, E64I, R70Q, R71N

Available Since

Aug. 20, 2018

Availability

Academic Institutions and Nonprofits only

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spNW100

Plasmid

#173488

Purpose

Expresses spNW100 in Bl21

Depositor

Huan Bao

Article

Zhang et al Nat Commun. 2021 Sep 14;12(1):5451. doi:

Insert

spNW100

Tags

Histag

Expression

Bacterial

Mutation

P1049L- please see depositor comments

Promoter

T7

Available Since

Sept. 15, 2021

Availability

Academic Institutions and Nonprofits only

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xCas9(3.7)-ABE(7.10)

Plasmid

#108382

Purpose

Mammalian expression vector for xCas9(3.7)-ABE

Depositor

David Liu

Article

Hu et al Nature. 2018 Feb 28. pii: nature26155. d

Insert

xCas9(3.7)-ABE(7.10)

Tags

NLS

Expression

Mammalian

Mutation

A262T, R324L, S409I, E480K, E543D, M694I, E1219V

Available Since

March 28, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3/HA-SUMO3 (Sentrin 2)

Plasmid

#17361

Purpose

Mammalian expression of human SUMO3 with HA tag

Depositor

Edward Yeh

Article

Kamitani et al J Biol Chem. 1998 Feb 6. 273(6):3117-20.

Insert

SUMO3 (SUMO3 Human)

Tags

HA

Expression

Mammalian

Available Since

April 4, 2008

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-SIO-eOPN3-mScarlet-WPRE

Plasmid

#125713

Purpose

Cre-dependent vector expressing an optimized mosquito OPN3 rhodopsin in-frame with mScarlet

Depositor

Ofer Yizhar

Article

Mahn et al Neuron. 2021 May 10. pii: S0896-6273(21)

Has Service

AAV1 and AAV5

Insert

eOPN3

Use

AAV and Cre/Lox

Tags

Rho1D4 and mScarlet

Expression

Mammalian

Mutation

deleted amino acids 331-429

Promoter

hSyn1

Available Since

Sept. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pKK-TEV-BirA-FLAG

Plasmid

#105786

Purpose

Expression of your protein of interest in fusion with BirA-FLAG at the C-terminus (cleavable by TEV). Search for protein partners using the BioID (PMID: 15707685, 22412018).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

TEV-BirA-FLAG

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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ES-FUCCI

Plasmid

#62451

Purpose

SMX_DEST vector with 3 cassettes

Depositor

Pierre Neveu

Article

Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. do

Inserts

minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
minPGK::Cherry-hCdt1(30-120)-bGHpA
PGK::HygroR-bGpA

Use

Synthetic Biology

Available Since

May 8, 2015

Availability

Academic Institutions and Nonprofits only

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pRF+423Dux4

Plasmid

#21625

Depositor

Stephen Tapscott

Article

Snider et al Hum Mol Genet. 2009 Jul 1;18(13):2414-30

Insert

Double homeobox, chr 4 (DUX4 Human)

Use

Luciferase

Available Since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
pSBtet-RN

Plasmid

#60503

Purpose

SB-transposon with inducible SfiI cloning site for GOI (contains firefly luciferase) and constitutive expression of RFP, rtTA and neomycin resistance gene

Depositor

Eric Kowarz

Article

Kowarz et al Biotechnol J. 2015 Feb 4. doi: 10.1002/b

Type

Empty backbone

Use

Transposon

Expression

Mammalian

Promoter

TCE & RPBSA

Available Since

Feb. 11, 2015

Availability

Academic Institutions and Nonprofits only

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mTagBFP2-Farnesyl-5

Plasmid

#55295

Purpose

Localization: Plasma Membrane, Excitation: 399, Emission: 456

Depositor

Michael Davidson

Article

Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

Insert

Farnesyl (HRAS Human)

Tags

mTagBFP2

Expression

Mammalian

Mutation

encodes final 20 aa of NM_001130442.1

Promoter

CMV

Available Since

Oct. 10, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pYD1-mSA

Plasmid

#39865

Depositor

Sheldon Park

Article

Lim et al Biotechnol Bioeng. 2013 Jan;110(1):57-67

Insert

monomeric streptavidin

Tags

Aga2, FLAG, and HA

Expression

Yeast

Available Since

March 15, 2013

Availability

Academic Institutions and Nonprofits only

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AAV-P(Per2)-DIO-intron2-dLUC

Plasmid

#110059

Purpose

Per2 transcription luciferase reporter

Depositor

Eric Erquan Zhang

Article

Mei et al Proc Natl Acad Sci U S A. 2018 Apr 2. pi

Insert

Per2 promoter and luciferase (Per2 Mouse)

Use

AAV and Cre/Lox

Expression

Mammalian

Promoter

Per2

Available Since

May 30, 2018

Availability

Academic Institutions and Nonprofits only

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GUM BUB3

Plasmid

#84029

Purpose

Expression of mCherry tagged BUB3 in human cells

Depositor

Patrick Paddison

Article

Toledo et al Dev Cell. 2014 Feb 10;28(3):282-94. doi:

Insert

BUB3 (BUB3 Human)

Use

Lentiviral

Tags

mCherry

Expression

Mammalian

Promoter

UCOE / SFFV

Available Since

Oct. 13, 2016

Availability

Academic Institutions and Nonprofits only

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Tom20-V5-FKBP-AP_pLX304

Plasmid

#120914

Purpose

Lentiviral vector expressing Tom20-V5-FKBP-AP on mammalian mitochondrial outer membrane

Depositor

Alice Ting

Article

Han et al ACS Chem Biol. 2019 Mar 8. doi: 10.1021/

Insert

Tom20-V5-FKBP-AP

Use

Lentiviral

Expression

Mammalian

Available Since

March 20, 2019

Availability

Academic Institutions and Nonprofits only

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pET21a-TrpRS-His

Plasmid

#124120

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Tryptophanyl-tRNA ligase (trpS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 19, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1+_Lambda-N-HA-Peptide

Plasmid

#92005

Purpose

Expresses Lambda-N-HA peptide

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

Lambda-N-HA peptide

Tags

Lambda-N-HA

Expression

Mammalian

Available Since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pET21a-LeuRS-His

Plasmid

#124113

Purpose

Bacterial expression plasmid for PURE system components

Depositor

Sebastian Maerkl, Takuya Ueda

Article

Shimizu et al Nat Biotechnol. 2001 Aug;19(8):751-5. do

Insert

Leucyl-tRNA ligase (leuS Escherichia coli)

Tags

6x-His

Expression

Bacterial

Available Since

April 4, 2019

Availability

Academic Institutions and Nonprofits only

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FLAG-HSF1

Plasmid

#32537

Depositor

Stuart Calderwood

Article

Wang et al Mol Cell Biol. 2003 Sep;23(17):6013-26.

Insert

HSF1 (HSF1 Human)

Tags

FLAG

Expression

Mammalian

Mutation

see depositor comments below

Promoter

CMV

Available Since

Oct. 6, 2011

Availability

Academic Institutions and Nonprofits only

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PLKO-mcherry-luc-puro-TRPM1 cDNA

Plasmid

#29782

Depositor

Carl Novina

Article

Levy et al Mol Cell. 2010 Dec 10. 40(5):841-9.

Insert

TRPM1 (TRPM1 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

July 29, 2011

Availability

Academic Institutions and Nonprofits only

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